Method for photochemically inducing macaque cerebral stroke model

A stroke and photochemical technology, applied in the field of medical animal model research, can solve the problems of unsustainable modeling effect, large trauma and high lethality in rhesus monkeys, and achieve the effect of reducing individual differences, small invasiveness and low lethality rate of rhesus monkeys

Pending Publication Date: 2020-08-04
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The current macaque middle cerebral artery embolism models include: 1. Craniotomy and middle cerebral artery clamping. This method is complicated to operate and requires certain surgical technicians to operate. It is easy to cause huge damage and has a high fatality rate.
2. Puncturing the middle cerebral artery embolization with a microcatheter through the macaque femoral artery under DSA. This method requires complex equipment, and the operator has a certain l

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  • Method for photochemically inducing macaque cerebral stroke model

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Embodiment 1

[0022] After selecting macaques that meet the criteria:

[0023] 1) Aseptic operation was maintained during the macaque operation. The surgical side was determined according to the preoperative behavior of the macaque. The Sylvian fissure area of ​​the brain region on the side opposite the dominant hand was exposed. The size of the bone window was 3.66±3.48 cm×2.75±2.67 cm. Then use an electroacupuncture instrument (Huatuo brand, Suzhou Medical Supplies Factory Co., Ltd.) to stimulate, determine the contralateral motor area that controls the right hand, and inject rose bengal (sigma, Shanghai Weiting Biotechnology Co., Ltd.) 20mg / kg through the femoral vein .

[0024] 2) Complete step 1) After 6 minutes of injection, use a green laser (Reward, Shenzhen Ruiward Co., Ltd.) to irradiate with a current of 1.25 amperes, 1,400,000 lux, a wavelength of 532 nm, and a spot diameter of 8 mm The cold green light source continuously irradiated the three parts of the macaque for 20 minute...

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Abstract

The invention relates to a method for photochemically inducing a macaque cerebral stroke model. The method comprises the following steps: (1) intravenously injecting 20mg/kg of rose-bengal; and (2) respectively continuously illuminating three parts at a far end of a middle cerebral artery of the macaque for 20 minutes. By utilizing the method for continuously carrying out photochemical induction on three brain parts of the macaque, the durably injured macaque cerebral stroke model is constructed, the operation is easy, a durable modeling effect is achieved, a macaque is slightly injured by themacaque cerebral stroke model, the fatality rate is low, and the method is used for researching pathogenesis and treatment strategies of the cerebral stroke and has important significances to research of preclinical experiments for the cerebral stroke.

Description

technical field [0001] The invention belongs to the technical field of medical animal model research, in particular to a method for photochemically inducing a rhesus monkey stroke model. Background technique [0002] 1 out of every 6 people in the world may suffer from a stroke in their lifetime. The prevalence of cerebral apoplexy in China is increasing rapidly, with high morbidity, high mortality and high disability rate. Stroke is the leading cause of death in the world, accounting for about 20% of all deaths. In addition to causing death, moderate and severe strokes are often accompanied by sequelae, such as motor and cognitive impairments, which bring great pain to patients and their families. Therefore, using experimental animals to prepare stroke models to study the prevention and treatment of stroke is one of the urgent issues in biology and even in the medical field. Most of the existing stroke models have been developed with rodents and other small animals, duri...

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2207/35A01K2227/106A01K2267/0375
Inventor 王建红王树国张志婷林郁刘克中杜玲丽徐玲李经辉
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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