Application of pichia kudriavzevii in aspect of degrading biogenic amines

A biogenic amine and yeast technology, applied in the field of food fermentation, can solve the problems of low degradation rate and few types of biogenic amine degradation strains, and achieve a good effect of reducing amine

Inactive Publication Date: 2020-08-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reported biogenic amine-degrading strain...

Method used

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  • Application of pichia kudriavzevii in aspect of degrading biogenic amines
  • Application of pichia kudriavzevii in aspect of degrading biogenic amines
  • Application of pichia kudriavzevii in aspect of degrading biogenic amines

Examples

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Embodiment 1

[0020] Example 1: Screening and Identification of Pichia Kudori of Pichia Pichia Pichia Pichia Kudori

[0021] Take fish sauces in different fermentation periods as samples, take 10g of fish sauce samples and add them to 190mL sterile water, mix well, then serially dilute, and spread on the lower medium (on the basis of MRS medium, add pyridoxal phosphate 0.05g / L , agar 18g / L, tryptophan, phenylalanine, histidine, lysine, tyrosine, arginine 5g / L each, pH 5.2) on a solid plate, cultivated at 37°C for 24h, poured The upper medium (bromocresol purple 0.06g / L, agar 20g / L, pH 5.2) was initially screened by color reaction, the purple was positive (i.e. amine-producing bacteria), and the yellow color was negative (potential bacteria). Aminobacteria), such as figure 1 shown. Select the negative single colony and repeat the streak separation on the plate, and repeat three times to obtain a purified single colony for the next step of degradation and identification of biogenic amines. ...

Embodiment 2

[0024] Example 2: Degradation of biogenic amines by Pichiakudriavzevii under medium conditions

[0025] Preparation of MMRS liquid medium: in MRS liquid medium, add mixed biogenic amines (histamine, tyramine, cadaverine, putrescine, tryptamine, phenylethylamine, octopamine, spermidine and spermine 50mg / L, natural pH).

[0026] The strains were inoculated in liquid MRS medium for activation, and cultured at 37° C. and 180 rpm for 24 hours. Inoculate (by volume) 2% into 50 mL of MMRS medium. After culturing at 37°C and 180rpm for 72 hours, measure the content of biogenic amines in the medium, and compare with the blank group to obtain the degradation rate of biogenic amines of the strain; see Table 1-3 for specific results.

[0027] Table 1 Comparison of peak areas before and after degradation of nine biogenic amines by Pichia kurdori strains

[0028]

[0029] Table 2 Concentration comparison before and after degradation of nine biogenic amines by Pichia kurdori strains

...

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Abstract

The invention discloses application of pichia kudriavzevii in the aspect of degrading biogenic amine, and belongs to the technical field of food fermentation. A pichia kudriavzevii strain is put intoan MRS culture medium; nine biogenic amines (histamine, tyramine, cadaverine, putrescine, tryptamine, phenylethylamine, octopamine, spermidine and spermine) can be degraded through 72 h fermentation,the total amine degradation rate can reach 88.24%, and the histamine degradation rate, the tyramine degradation rate, the cadaverine degradation rate and the putrescine degradation rate are 91.97%, 93.24%, 78.58% and 96.03% respectively. The strain provided by the invention can degrade various biogenic amines at the same time, shows good amine reducing capacity in an MRS culture medium, and has important significance for controlling the biogenic amines in food.

Description

technical field [0001] The invention relates to the application of Pichia kurdoris in degrading biogenic amines, and belongs to the technical field of food fermentation. Background technique [0002] Biogenic amines are a class of biologically active nitrogen-containing low-molecular-weight organic compounds, which are mainly produced by microbial amino acid decarboxylases and are ubiquitous in foods, especially fermented foods. Biogenic amines can be regarded as substances produced by substituting 1-3 hydrogen atoms in nitrogen atoms with alkyl or aryl groups. They are low-molecular-weight organic bases of aliphatic, alicyclic or heterocyclic groups, and often exist in plants and animals. In vivo and in fermented foods. An appropriate amount of biogenic amines can promote human growth, enhance metabolic activity, enhance immunity and scavenge free radicals, etc. Excessive intake of exogenous biogenic amines will disrupt the balance of biogenic amines in the body, mainly ca...

Claims

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Application Information

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IPC IPC(8): A23L5/20C12N1/16C12R1/84
CPCA23L5/28A23V2002/00C12N1/16A23V2250/76
Inventor 徐莹朱翠翠王海英汪东风孙逊赵玉莹舒丹旭
Owner OCEAN UNIV OF CHINA
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