Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of stem cell composition and its application and stem cell drip

A composition and stem cell technology, applied in the field of stem cell drip and stem cell composition, can solve the problems of infection, necrosis, affecting the health and quality of life of patients, and achieve the effect of rich sources

Active Publication Date: 2022-01-25
SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Trauma, burns, chronic ulcers, etc. cause large areas of skin defects, often forming wounds that are difficult to heal. Due to the slow healing, they often bring risks such as necrosis and infection, seriously affecting the health and quality of life of patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of stem cell composition and its application and stem cell drip
  • A kind of stem cell composition and its application and stem cell drip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of clinical-grade HUC-MSC preparations:

[0030] 1.1 Primary clinical grade HUC-MSC isolation:

[0031] 1.1.1 Check the experimental environment and the operation of instruments and equipment before the experiment starts. Wipe and disinfect the operating table top with 75% ethanol solution, and wipe and disinfect the utensils and supplies with high pressure or alcohol. Prepare the supplies needed for the experiment.

[0032] 1.1.2 Both ends of the umbilical cord tissue were ligated, soaked in a normal saline bottle containing double antibody (article number: 15140122, Gibco), and transported to the laboratory at 2-8°C. Open the bottle in the ultra-clean work counter, discard the preservation solution in the bottle, add physiological saline (just immerse the umbilical cord) into the bottle, and shake and wash.

[0033] 1.1.3 After discarding the saline, pour the umbilical cord into a sterile 100mm petri dish, and spray the surface of the umbilical cord ...

Embodiment 2

[0074] Preparation of stem cell product drops that help skin trauma healing:

[0075] The 1×10 prepared in Example 1 7 HUC-MSC (0.5mL PBS) and 1×10 7 HUVEC (0.5mL PBS) were mixed evenly, divided into 0.1mL tubes and stored on ice or at 4°C for later use to obtain stem cell product drops.

Embodiment 3

[0077] 1. Experimental method

[0078] 1. Take 1.4 × 10 by the method of Example 1 (1.5 step HUC-MSC suspension preparation) method 7 A P5 HUC-MSC was resuspended in 0.7mL PBS (in a 1.5mL EP tube) and placed on ice for use;

[0079] 2. Get 7 × 10 by Example 1 (2.4 step preparation of HUVEC suspension) 6 A P5 generation HUVEC was resuspended in 0.7mL PBS (in a 1.5mL EP tube) and placed on ice for use;

[0080] 3. Divide the experiment into 3 treatment groups: PBS, MSC and MSC+VEC groups. 0.1mL PBS per tube in PBS group, 1×10 per tube in MSC group 6 MSC / 0.1mL PBS, MSC+VEC group 1×10 per tube 6 MSC and 1×10 6 Each VEC / 0.1mL PBS, and each group made 7 repetitions. Divide the cell mixture prepared in the above steps 1 and 2 according to the grouping.

[0081] 4. The animal experiment was carried out in the ultra-clean bench in the animal room. Before the experiment, the operating table was wiped and disinfected with 75% ethanol solution, and the ophthalmic scissors and tweezer...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a stem cell composition and its application and stem cell drops, which belong to the technical field of skin trauma repair. The stem cell composition includes human umbilical cord mesenchymal stem cells and human umbilical cord vascular endothelial cells; the human umbilical cord mesenchymal The ratio of stem cells to human umbilical cord vascular endothelial cells is 0.5-1.5:0.5-1.5. The skin trauma can be healed by using the stem cell composition provided by the invention, and the healing time only needs 13 days.

Description

technical field [0001] The invention belongs to the technical field of skin trauma repair, and in particular relates to a stem cell composition, an application thereof, and a stem cell drop. Background technique [0002] Trauma, burns, chronic ulcers, etc. cause large areas of skin defects, often forming wounds that are difficult to heal. Due to the slow healing, they often bring risks such as necrosis and infection, which seriously affect the health and quality of life of patients. Wound repair is a complex and orderly biological process, including the hemostatic phase, inflammatory phase, proliferative phase, remodeling phase and other continuous processes, involving the participation and mutual coordination of various cells, cytokines, and growth factors. Factors such as hypoxia, necrosis, infection, and advanced age can delay or make it difficult to heal wounds. Therefore, reconstruction and functional restoration are still a major problem in the field of tissue repair. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/44A61K35/28A61P17/02C12N5/071C12N5/0775
CPCA61K35/44A61K35/28A61P17/02C12N5/0665C12N5/069A61K2300/00
Inventor 刘中民贾文文齐奕尧汤红明
Owner SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com