Improved chicken-derived antibacterial peptide as well as encoding gene, recombinant plasmid, recombinant bacterium and application thereof

A technology of recombinant plasmids and encoding genes, applied in the field of genetic engineering, can solve the problems of low antibacterial ability of natural antibacterial peptides and limit the wide application of antibacterial peptides, and achieve the effect of enhanced antibacterial ability and strong antibacterial ability.

Active Publication Date: 2020-08-18
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural antimicrobial peptides are not perfect, and most of them have low ant

Method used

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  • Improved chicken-derived antibacterial peptide as well as encoding gene, recombinant plasmid, recombinant bacterium and application thereof
  • Improved chicken-derived antibacterial peptide as well as encoding gene, recombinant plasmid, recombinant bacterium and application thereof
  • Improved chicken-derived antibacterial peptide as well as encoding gene, recombinant plasmid, recombinant bacterium and application thereof

Examples

Experimental program
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Effect test

Example Embodiment

[0028] Example 1

[0029] 1. The modified modified chicken-derived antimicrobial peptide DNA sequence introduces restriction site

[0030] Improve the coding genes of antimicrobial peptides (first gene SEQ ID NO.4 and second gene SEQ ID NO.5), with restriction enzyme cutting sites Xho Ⅰ introduced at the 5'ends respectively Then introduce the DNA sequence of Kex2 restriction site (amino acid restriction site EKR) 3′ introduced stop codon TGA, plus Xba Ⅰ Restriction sites. The designed sequence is submitted to DNA synthesis company (Nanjing GenScript Biotechnology Co., Ltd.) for synthesis. The synthesized DNA sequence is connected to the plasmid pUC57-Simple (the mass spectrum is as shown in figure 1 Shown) on.

[0031] The nucleotide sequence after the first gene is introduced into the restriction site is shown in SEQ ID NO. 6, specifically:

[0032]

[0033] The nucleotide sequence after the second gene is introduced into the restriction site is shown in SEQ ID NO. 7, specifically...

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PUM

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Abstract

The invention provides an improved chicken-derived antibacterial peptide, and a coding gene, a recombinant plasmid, a recombinant bacterium and an application thereof, which belong to the technical field of gene engineering, the improved chicken-derived antibacterial peptide comprises a first antibacterial peptide and/or a second antibacterial peptide; the amino acid sequence of the first antibacterial peptide is as shown in SEQ ID NO. 2; and the amino acid sequence of the second antibacterial peptide is shown as SEQ ID NO. 3. The improved chicken-derived antibacterial peptide disclosed by theinvention is an improved chicken-derived antibacterial peptide designed on the basis of an original chicken-derived antibacterial peptide (the amino acid sequence is shown as SEQ ID NO.1). Compared with the original chicken-derived antibacterial peptide, the improved chicken-derived antibacterial peptide disclosed by the invention has the advantage that the antibacterial capability is obviously enhanced.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to improved chicken-derived antimicrobial peptides and their coding genes, recombinant plasmids, recombinant bacteria and applications. Background technique [0002] Antimicrobial peptides are biologically active small molecule polypeptides induced in organisms, usually composed of 12 to 45 amino acids, with a molecular weight of about 40kD, and are not easy to make bacteria resistant to drugs; in addition, they also have broad-spectrum antibacterial properties, Thermal stability, high water solubility and no residue. Therefore, the study and application of antimicrobial peptides as non-toxic, non-polluted, non-drug-resistant new antibacterial agents to replace antibiotics is of great significance for the protection of human health. However, natural antimicrobial peptides are not perfect, and most of them have low antibacterial ability, which greatly limits the wide app...

Claims

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Application Information

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IPC IPC(8): C07K14/465C12N15/12C12N15/81C12N1/19A61K38/17A61P31/04C12R1/84
CPCC07K14/465C12N15/815A61P31/04A61K38/00
Inventor 王东升张国华黄江丽圣平王汝霞张志红于一尊计少石
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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