Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Improved chicken-derived antibacterial peptide as well as encoding gene, recombinant plasmid, recombinant bacterium and application thereof

A technology of recombinant plasmids and encoding genes, applied in the field of genetic engineering, can solve the problems of low antibacterial ability of natural antibacterial peptides and limit the wide application of antibacterial peptides, and achieve the effect of enhanced antibacterial ability and strong antibacterial ability.

Active Publication Date: 2020-08-18
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural antimicrobial peptides are not perfect, and most of them have low antibacterial ability, which greatly limits the wide application of antimicrobial peptides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Improved chicken-derived antibacterial peptide as well as encoding gene, recombinant plasmid, recombinant bacterium and application thereof
  • Improved chicken-derived antibacterial peptide as well as encoding gene, recombinant plasmid, recombinant bacterium and application thereof
  • Improved chicken-derived antibacterial peptide as well as encoding gene, recombinant plasmid, recombinant bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. The modified chicken-derived antimicrobial peptide DNA sequence was introduced into the enzyme cutting site

[0030] The coding gene of the improved antimicrobial peptide (the first gene SEQ ID NO.4 and the second gene SEQ ID NO.5), the 5' ends were respectively introduced into the restriction enzyme site Xho Ⅰ Reintroduce the DNA sequence of the Kex2 restriction site (amino acid restriction site EKR) 3' introduced stop codon TGA, plus Xba Ⅰ Restriction sites. The designed sequence was submitted to a DNA synthesis company (Nanjing KingScript Biotechnology Co., Ltd.) for synthesis, and the synthesized DNA sequence was connected to the plasmid pUC57-Simple (mass spectrum as shown in figure 1 shown) above.

[0031] The nucleotide sequence of the first gene after introducing the restriction site is shown in SEQ ID NO.6, specifically:

[0032]

[0033] The nucleotide sequence of the second gene after introducing the restriction site is shown in SEQ ID NO.7, specif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an improved chicken-derived antibacterial peptide, and a coding gene, a recombinant plasmid, a recombinant bacterium and an application thereof, which belong to the technical field of gene engineering, the improved chicken-derived antibacterial peptide comprises a first antibacterial peptide and / or a second antibacterial peptide; the amino acid sequence of the first antibacterial peptide is as shown in SEQ ID NO. 2; and the amino acid sequence of the second antibacterial peptide is shown as SEQ ID NO. 3. The improved chicken-derived antibacterial peptide disclosed by theinvention is an improved chicken-derived antibacterial peptide designed on the basis of an original chicken-derived antibacterial peptide (the amino acid sequence is shown as SEQ ID NO.1). Compared with the original chicken-derived antibacterial peptide, the improved chicken-derived antibacterial peptide disclosed by the invention has the advantage that the antibacterial capability is obviously enhanced.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to improved chicken-derived antimicrobial peptides and their coding genes, recombinant plasmids, recombinant bacteria and applications. Background technique [0002] Antimicrobial peptides are biologically active small molecule polypeptides induced in organisms, usually composed of 12 to 45 amino acids, with a molecular weight of about 40kD, and are not easy to make bacteria resistant to drugs; in addition, they also have broad-spectrum antibacterial properties, Thermal stability, high water solubility and no residue. Therefore, the study and application of antimicrobial peptides as non-toxic, non-polluted, non-drug-resistant new antibacterial agents to replace antibiotics is of great significance for the protection of human health. However, natural antimicrobial peptides are not perfect, and most of them have low antibacterial ability, which greatly limits the wide app...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/465C12N15/12C12N15/81C12N1/19A61K38/17A61P31/04C12R1/84
CPCC07K14/465C12N15/815A61P31/04A61K38/00
Inventor 王东升张国华黄江丽圣平王汝霞张志红于一尊计少石
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com