Sulindac and naproxen immunoassay card with marker guiding signal amplification effect

A technology for guiding signals and sulindac, applied in the field of immunodetection cards of sulindac and naproxen, can solve the problems of unstable quality, low sensitivity, weak detection signal strength, etc., to improve detection sensitivity and quantitative precision , Improve the detection signal strength and sensitivity, increase the effect of signal strength and stability

Pending Publication Date: 2020-08-21
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has the following disadvantages: 1. To detect different target substances, it is necessary to use corresponding detection antibodies to connect quantum dot microspheres to prepare fluorescent probes, which requires a large workload, low yield, and unstable quality; The reaction to prepare fluorescent probes may affect the structure and immunoaffinity of antibodies; 3. Lack of signal amplification mechanism, the detection signal intensity is weak and the sensitivity is low
However, the immunogenicity of the "detection antigen carrier protein" is unstable, and slight changes in protein conformation will lead to a decrease in the affinity between the "universal antibody" and the "detection antigen", weaken the formation of "sandwich immune complexes", and affect the detection signal. produce

Method used

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  • Sulindac and naproxen immunoassay card with marker guiding signal amplification effect
  • Sulindac and naproxen immunoassay card with marker guiding signal amplification effect
  • Sulindac and naproxen immunoassay card with marker guiding signal amplification effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] An immunoassay card for sulindac and naproxen that guides the signal amplification effect of a marker, comprising a liner 5 and bifunctional antigen release pads 1 that are sequentially adhered to the liner 5 and partially overlapped adjacent to each other , a universal fluorescent probe release pad 2, a nitrocellulose membrane 3 (chromatographic membrane) and a water-absorbent pad 4; The detection antigen is based on the coupling of "7-amino-4-hydroxy-2-naphthalenesulfonic acid" label 62; the universal fluorescent probe release pad 2 contains anti-"7-amino-4-hydroxy-2 - the universal fluorescent probe of naphthalenesulfonic acid antibody 65, the universal fluorescent probe is quantum dot 61; the nitrocellulose membrane 3 is provided with detection line A, detection line B and quality control line 33, the detection line A, detection line B respectively immobilized anti-sulindac antibody 311 and anti-naproxen antibody 321, and the quality control line 33 immobilized anti...

Embodiment 2

[0050] Simultaneously detect the detection method of sulindac and naproxen, the steps are as follows:

[0051] Take 0.2g (or 0.2mL) sample, dissolve it in 2.0mL absolute ethanol to fully extract the target sample, let the extract stand for 10 minutes to fully precipitate impurities, take 0.2mL supernatant and add it to 20mL sample buffer, fully Shake well to form a sample solution; drop 3-4 drops of sample solution on the bifunctional antigen release pad with a dropper, and the bifunctional antigen and universal fluorescent probe are released during the movement of the sample solution to the nitrocellulose membrane, and pass through the detection area successively. Line and quality control line, according to the fluorescent signal of detection line A, detection line B and quality control line to determine whether the sample contains sulindac and naproxen.

[0052] refer to Figure 5. The anti-sulindac antibody 311 immobilized on the detection line A of the nitrocellulose mem...

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Abstract

The invention relates to a sulindac and naproxen immunoassay card with a marker guiding signal amplification effect. The sulindac and naproxen immunoassay card comprises a lining plate, and a difunctional antigen release pad, a universal fluorescent probe release pad, a chromatographic membrane and a water absorption pad which are sequentially adhered to the lining plate and are partially overlapped with one another, wherein the bifunctional antigen release pad comprises a specific marker coupled on the basis of a detection antigen formed by coupling ovalbumin with sulindac and naproxen respectively; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with an anti-specific marker antibody; the chromatographic membrane is provided with a detection line A, a detection line B and a quality control line which are respectively used for fixing an anti-sulindac antibody, an anti-naproxen antibody and an anti-specific marker antibody. According to the invention, the production of all detection reagents can be solved by using the universal fluorescent probe, the cost is reduced, and the quality control reliability is improved; and the signal intensityand the stability of detection are improved, and the detection sensitivity and the quantitative precision are improved.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to an immunological detection card for sulindac and naproxen with a marker-guided signal amplification effect. Background technique [0002] Both sulindac and naproxen are non-steroidal anti-inflammatory drugs with anti-inflammatory, analgesic and antipyretic effects. Because these two kinds of analgesic effects are quick and cheap, unscrupulous manufacturers illegally add sulindac and naproxen to health food (mainly health wine) for rheumatic diseases to enhance the curative effect and seek illegitimate benefits. These two non-steroidal anti-inflammatory drugs are prescription drugs. Improper use may cause damage to the digestive tract, liver and kidneys, and even lead to anaphylactic shock and acute renal failure in severe cases. The long-term use of health food containing sulindac and naproxen without the knowledge of the patient is very harmful. It is necessary to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/558
CPCG01N33/9486G01N33/588G01N33/582G01N33/558
Inventor 郭杰标
Owner SHAOGUAN COLLEGE
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