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Preparation method of adipose tissue extracellular matrix

An adipose tissue and extramatrix technology, which can be used in tissue regeneration, medical science, prosthesis, etc., can solve the problems of increasing the preparation cost and the high price of biological enzymes, and achieve the effect of reducing the preparation cost, retaining collagen components, and saving environmental protection.

Active Publication Date: 2020-09-01
PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the one hand, biological enzymes are expensive, which greatly increases the production cost; on the other hand, since the above enzymes are all derived from heterogeneous species such as cattle or pigs, this increases the introduction of Xenoantigen Risk

Method used

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  • Preparation method of adipose tissue extracellular matrix
  • Preparation method of adipose tissue extracellular matrix
  • Preparation method of adipose tissue extracellular matrix

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Experimental program
Comparison scheme
Effect test

Embodiment

[0083] The preparation method of the adipose tissue extracellular matrix of the present embodiment comprises the following steps:

[0084] Step 1: Pretreatment of Adipose Tissue

[0085] Take fresh adipose tissue, let it stand for 10min-15min, discard oil droplets, blood and swelling fluid, add PBS buffer solution to wash 3 times, and obtain pretreated adipose tissue (such as figure 1 shown).

[0086] Step 2: Freeze-Thaw Cycle

[0087] The pretreated adipose tissue obtained in step 1 was frozen at -80° C. for 2 hours, and then thawed at 37° C.; the above freezing and thawing process was performed 3 times in total to obtain the adipose tissue after freeze-thawing treatment.

[0088] Step 3: Broken

[0089] The adipose tissue obtained in step 2 after freezing and thawing is broken into a chylus shape, and many ways can be used for breaking, such as mortar method, smashing method, oscillation method, stirring method, homogenization method, temperature difference method, pressu...

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Abstract

The invention discloses a preparation method of an adipose tissue extracellular matrix, and belongs to the technical field of tissue engineering, regenerative medicine and clinical medicine. The preparation method comprises the following steps: taking fresh adipose tissues, carrying out pretreating, and carrying out freeze thawing treatment; crushing the adipose tissue subjected to freeze thawingtreatment to obtain crushed adipose tissues; and then sequentially carrying out high-permeability sodium chloride solution cleaning, first-time sterile deionized water washing, TritonX-100 solution cleaning, second-time sterile deionized water washing, isopropanol grease precipitation, third-time sterile deionized water washing and alcohol RNA elution to obtain the adipose tissue extracellular matrix. Enzyme participation is not needed in the decellularization process, so that the preparation cost is reduced, the risk of rejection reaction caused by heterologous antigen components is reduced,the natural structure and active factors of extracellular matrixes can be reserved, more effective decellularization results are achieved, and the in-vivo adipogenesis capacity is higher.

Description

technical field [0001] The invention relates to a method for preparing extracellular matrix of adipose tissue, belonging to the technical fields of tissue engineering, regenerative medicine and clinical medicine. Background technique [0002] Tissue defects caused by trauma, tumors, congenital malformations and aging are the focus of plastic surgery. Autologous tissue transplantation and tissue engineering material transplantation are two main strategies for repairing tissue defects. Although autologous tissue transplantation can achieve ideal therapeutic effects, the price paid is large surgical trauma and scars left in the donor site, and even serious complications may occur. Tissue engineering material transplantation has the characteristics of simple operation and less trauma, and has unique advantages in tissue transplantation. The three elements of tissue engineering are tissue engineering materials, seed cells and growth factors. Among them, scaffold materials are o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3633A61L27/3687A61L27/3691A61L2430/40
Inventor 祁珺栾杰
Owner PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
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