Cancer gene detection kit based on second-generation sequencing probe capture method

Abstract

The invention discloses a cancer gene detection kit based on a second-generation sequencing probe capture method. The cancer gene detection kit comprises quality control materials, library building reagents and an RNA probe; quality control materials: a negative quality control material and a positive quality control material; library building reagents: a tail end repairing and A adding reagent, ajoint adding reagent, an amplification reagent, a hybridization reagent, a capture reagent and an amplification reagent; the RNA probe is a probe panel; the synthesis type of the RNA probe is an RNAsingle strand; the length interval is 90-120 nt; and biotin is marked. The cancer gene detection kit in the invention aims at patients with lung cancer, colorectal cancer, breast cancer and ovarian cancer. Detection of 18 genes panel is carried out; the receptivity of medicines, such as targeting and chemotherapy, on a patient is comprehensively interpreted from the gene level; according to the difference of individual gene levels, an accurate personalized treatment scheme is made; immune medication treatment is guided; the sensitivity and toxic and side effects of chemotherapy drugs are predicted; a medication prognosis condition is dynamically monitored, etc.

Description

technical field [0001] The invention belongs to the field of gene detection kits, in particular to a cancer gene detection kit based on a second-generation sequencing probe capture method. Background technique [0002] Since the emergence of the first-generation sequencing technology (Sanger method) in 1977, the sequencing technology has developed to the fourth generation, but in terms of the current application scenarios and situation, the second-generation sequencing technology is still the mainstream. The core principle of next-generation sequencing technology, in short, is sequencing while synthesizing, and determining the DNA sequence by capturing the markers of the newly synthesized ends; it has the characteristics of high throughput, short read length, and fast cycle. At present, the main suppliers of second-generation sequencers are foreign Illumina and Life Tech, and domestic sequencers made by MGI are also gradually rising. [0003] The complete process of next-ge...

AI Technical Summary

Problems solved by technology

The advantage of this product is that it can formulate precise personalized treatment plan, can be used in liquid biopsy, and can dynamically monitor the results after medication; the disadvantage is that the detection of genes is limited, and the cancer type is too single

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