Small-sized fermentation system and fermentation method capable of simulating colon environment

Abstract

The invention relates to a small-sized fermentation system and fermentation method capable of simulating a colon environment and a fermentation method thereof, and belongs to the technical field of microbiology, human body physiology and bionics. The small-sized fermentation system capable of simulating the colon environment comprises a material supplementation bottle, a fermentation main body anda sample collection device; the material supplementation bottle is provided with a gas inlet; the fermentation main body comprises three fermentation branch port test tubes; the fermentation branch port test tubes are all provided with gas collection ports; and the sample collection device comprises three sample collectors. The fermentation method capable of simulating the colon environment usesthree fermentation branch port test tubes to respectively simulate ascending colon, transverse colon and descending colon and carry out a fermentation reaction, and a time period is 7d. The small-sized fermentation system has the following beneficial effects that the entire system implements simple simulation of colon fermentation, and has excellent stability, practicality and repeatability. Modules are simple, cost is low, the adopted device is simple; a plurality of small-sized fermentation systems can be simultaneously set up to carry out pre-experiment on different samples; parallelism ishigh; and after fermentation is ended, collected gas samples and liquid samples can be used for subsequent detection.

Description

technical field [0001] The invention belongs to the technical fields of microbiology, human physiology and bionics, and specifically relates to a small-scale fermentation system and a fermentation method for simulating colon environment. Background technique [0002] As the largest and most complex micro-ecological system in the human body, the intestinal flora itself and its metabolites can not only regulate human health, but also play an important role as a bridge between the diet and the host. Research has profound implications for human health. [0003] Due to the harm of animal experiments to the tested animals and the differences in the composition of intestinal microbes in the existing animal experiments from those of the human body, the experimental results cannot well reflect the metabolism of the human body and the data of the intestinal microbiota. The intestinal in vitro digestion simulation system is widely used in the in vitro evaluation of food and pharmaceut...

AI Technical Summary

Problems solved by technology

[0003] Due to the harm of animal experiments to the tested animals and the differences in the composition of intestinal microbes in the existing animal experiments from those of the human body, the experimental results cannot well reflect the metabolism of the human body and the data of the intestinal microbiota. Intestinal in vitro digestion simulation system is widely used in in vitro evaluation of food and pharmaceuticals

In the past ten years, the intestinal in vitro digestion simulation system has been continuously developed and innovated, and the intestinal in vitro digestion simulation system has been widely used at home and abroad to carry out research on the digestion and evaluation of food intestinal microorganisms. Taking SHIME as an example, it has 6 The fermentation tank simulates the stomach, duodenum, jejunum, ileum, ascending colon, transverse colon, and descending colon. This system is recognized by experts all over the world. Long time (about 15 days for one experiment), large volume, high cost, and high operating requirements, the most important thing is that only one sample can be evaluated at a time, so we need to conduct a pre-experiment first to evaluate whether there is a need for a complete evaluation

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