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SNP molecular markers related to barley grain β-glucan content and their application

A technology of molecular markers and dextran, which is applied in the fields of molecular biology and genetic breeding, can solve the problems of non-specificity of β-glucan, high price of kits, and low measurement results, so as to improve identification efficiency and accuracy High degree, convenient and stable amplification, accurate and efficient detection

Active Publication Date: 2021-10-08
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the phenol-sulfuric acid method has no specificity for β-glucan because polysaccharides and oligosaccharides are hydrolyzed into monosaccharides by sulfuric acid, and the measurement results have large errors; the Congo red method is mostly used for beer β-glucan content. Too low; although the double-enzyme method and the improved double-enzyme method have high specificity and accuracy, the determination time is long, and the price of the kit used is high, and the determination cost is high

Method used

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  • SNP molecular markers related to barley grain β-glucan content and their application
  • SNP molecular markers related to barley grain β-glucan content and their application
  • SNP molecular markers related to barley grain β-glucan content and their application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Test materials

[0028] Using 100 global core barley germplasm resources as materials, they were planted and harvested in Hangzhou and Ningbo from 2018 to 2019, and the seeds were dried and stored for later use.

[0029] (2) Determination of traits

[0030] Grind the above-mentioned harvested seeds into powder with a sample grinder, and place them in a desiccator for dry storage for subsequent content determination. The β-glucan determination kit of Megazyme Company is used to determine the grain β-glucan of 100 parts of barley materials content.

[0031] (3) GWAS analysis and SNP molecular marker determination

[0032] Combined with the β-glucan content determined above and 279,515 SNP markers in this population, TASSEL software was used for GWAS analysis. The results showed that a SNP marker located on chromosome 6 was significantly associated with barley grain β-glucan content. The SNP is located at base 566409962 of chromosome 6 and can be detected repeatedly...

Embodiment 2

[0034] (1) Test materials

[0035] Using 20 global core germplasm resources as materials, the determination of grain β-glucan content and the analysis of S6H_566409962-SNP target region were carried out respectively. Specifically, as shown in Table 1, it includes 10 parts of high β-glucan material and 10 parts of low β-glucan material.

[0036] Table 1 20 barley germplasm materials with different β-glucan contents

[0037]

[0038]

[0039] (2) Acquisition of SNP markers

[0040] According to the above SNP site information, combined with the barley genome sequence information, SNP marker primers were developed. The upstream primer F is: 5'-CCGACTTCGTCTACGTGCTC-3'; the downstream primer R is: 5'-GGAGTTGCGGTACTTGTCGG-3', and the amplification size is 98bp , the SNP is located at the 47th bp of the amplified fragment.

[0041] Using the above primer pair and the following technique, the variation of the base at 566409962 on chromosome 6 of barley is detected, so as to re...

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Abstract

The invention discloses a SNP molecular marker related to barley grain β-glucan content and its application. The site of the SNP molecular marker is located at base number 566409962 of chromosome 6 of barley; the SNP base difference is C or T. The present invention discloses for the first time a SNP molecular marker significantly associated with barley grain β-glucan content. The molecular marker is accurate and efficient in detection, convenient and stable in amplification, and can be used for molecular marker-assisted selection to increase barley β-glucan content identification efficiency and accuracy.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and genetic breeding, in particular to a SNP molecular marker related to barley grain beta-glucan content and application thereof. Background technique [0002] Barley (Hordeum vulgare L.) is the fourth largest cereal crop in the world after corn, wheat and rice in planting area and output. It is widely used, mainly for food, feed and beer brewing. Compared with cereal crops such as corn, wheat and rice, the chemical composition of barley grain is unique, and the richness of β-glucan is one of its main characteristics. β-glucan is a polysaccharide in the grain cell wall of cereal plants, and is the main component of the grain cell wall. It is an important quality trait in malting barley, feeding barley and edible barley. When malting and brewing beer, dextran is an undesirable trait, which increases the viscosity of wort, makes filtration difficult, and easily makes beer turbid or prec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 叶玲珍耿腊李梦迪张国平
Owner ZHEJIANG UNIV
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