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Test strip for detecting endosulfan and application of test strip

A test strip and endosulfan technology is applied in the detection of endosulfan residues in vegetables and fruits, and in the field of colloidal gold test strips. The effect of short time and low cost

Active Publication Date: 2020-10-09
BEIJING KWINBON BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drug has a long validity period, bioaccumulation effect, great harm to fish, shellfish, etc., and cumulative toxicity to mammals

Method used

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  • Test strip for detecting endosulfan and application of test strip
  • Test strip for detecting endosulfan and application of test strip
  • Test strip for detecting endosulfan and application of test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 endosulfan detection test strip

[0037] The preparation method of this test strip mainly comprises the following steps:

[0038] 1) Prepare a conjugate release pad sprayed with endosulfan monoclonal antibody-colloidal gold marker;

[0039] 2) preparing a reaction membrane with a detection line coated with endosulfan hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;

[0040] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test strip.

[0041] The following step-by-step detailed description:

[0042] 1. Preparation of endosulfan hapten

[0043] Take 3.6g of endosulfan, add 100ml of 1,4-dioxane to dissolve, stir well, then add 3.0g of molecular sieve, add 1.02g of succinic semialdehyde, stir well, pass in hydrochloric acid gas, and stir at room temperature for 5h in t...

Embodiment 2

[0075] The detection of endosulfan residue in the sample of embodiment 2

[0076] 1. Test with test strips

[0077] Use a straw to absorb the sample solution to be tested and add 3 drops vertically to the sample hole, start timing when the liquid flows, react for 5-10 minutes, and judge the result.

[0078] 2. Analyze test results

[0079] Read the result by colloidal gold analyzer (referred to as "analyzer"):

[0080] Negative (-): Indicates that the concentration of the substance to be tested in the sample is lower than the detection limit;

[0081] Positive (+): Indicates that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit;

[0082] Invalid: Indicates that retesting is required.

Embodiment 3

[0083] Example 3 sample detection example

[0084] 1. Detection limit test

[0085] Take blank cucumber and apple samples, add endosulfan to them to the final concentration of 50, 100, and 200 μg / kg, respectively, take test strips for detection, and repeat the measurement three times for each sample.

[0086] When testing cucumber and apple samples with test strips, when the concentration of endosulfan was 50 μg / kg, the analyzer showed negative; when the concentration of endosulfan was 100 and 200 μg / kg, the analyzer showed positive.

[0087] 2. False positive rate and false negative rate test

[0088] Take 20 positive samples of cucumber and apple with known endosulfan content of 100 μg / kg and 20 negative samples of cucumber and apple with content less than 50 μg / kg, test with three batches of test strips, and calculate the negative and positive rates. The results are shown in Table 1 and Table 2.

[0089] Table 1 Cucumber test sample results

[0090]

[0091] Table 2 ...

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Abstract

The invention discloses a test strip for detecting endosulfan and application of the test strip. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction film (3),a water absorption pad (4) and a bottom plate (7), the reaction film is provided with a detection line (5) coated with a endosulfan hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse anti-antibody, and the conjugate release pad (2) is sprayed with an endosulfan monoclonal antibody-colloidal gold marker. The invention also provides a method for detectingendosulfan residues in vegetables and fruits such as cucumbers and apples by using the endosulfan test strip. The test strip provided by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

Description

technical field [0001] The invention relates to a test strip for detecting endosulfan and an application thereof, in particular to a colloidal gold test strip for detecting endosulfan, which is particularly suitable for the detection of endosulfan residues in vegetables and fruits such as cucumbers and apples. Background technique [0002] Endosulfan is a synthetic organochlorine compound widely used as a pesticide insecticide. Because the drug has the advantages of wide insecticidal range, low price, and low toxicity to bees, it is widely used in various crops. my country began to produce endosulfan in the 1990s. By 2011, there were 47 registered endosulfan products, involving 43 pharmaceutical companies, making it the second largest producer of the drug. The drug has a long period of validity, has bioaccumulation effect, is relatively harmful to fish and shellfish, and has cumulative toxicity to mammals. A large number of literatures have reported the detection of endosu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/543G01N33/532G01N33/53
CPCG01N33/577G01N33/558G01N33/543G01N33/532G01N33/5308G01N2430/10Y02A50/30
Inventor 吴小胜贾芳芳崔海峰万宇平何方洋吴广甘桂湘
Owner BEIJING KWINBON BIOTECH
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