Lactobacillus ZJUIDS03 for antagonizing helicobacter pylori and application of lactobacillus ZJUIDS03

A Helicobacter pylori and antagonism technology, applied in the field of microorganisms, can solve problems such as poor inhibition/inactivation effect of Helicobacter pylori, and achieve the effects of good probiotic properties, no antibiotic resistance, and inhibition of harmful pathogenic bacteria in the intestines

Active Publication Date: 2020-10-30
思我特(杭州)食品科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the existing Lactobacillus paracasei (Lactobacillus Paracasei), such as Lactobacillus paracasei K56 of Inner Mongolia Dairy Technology Research Institute Co., Ltd., Lactobacillus paracasei ZFM54 of Zhejiang Technology and Business University, and cheese from the Microbiology Laboratory of the Institute of Modern Physics, Chinese Academy of Sciences Lactobacillus JY, etc., the cells and products of the above-mentioned Lactobacillus paracasei have poor inhibition / inactivation effects on the growing Helicobacter pylori

Method used

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  • Lactobacillus ZJUIDS03 for antagonizing helicobacter pylori and application of lactobacillus ZJUIDS03
  • Lactobacillus ZJUIDS03 for antagonizing helicobacter pylori and application of lactobacillus ZJUIDS03
  • Lactobacillus ZJUIDS03 for antagonizing helicobacter pylori and application of lactobacillus ZJUIDS03

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, screening and identification of Lactobacillus paracasei ZJUIDS03:

[0056] 1. Screening of Lactobacillus paracasei ZJUIDS03

[0057] 1.1 Sample source

[0058] The bacterial strains used in the present invention are all collected from 10 copies of traditional fermented products in Yunnan --- farmhouse-made milk fans; that is, the present invention uses farmhouse-made milk fans from Yunnan as samples.

[0059] 1.2 Preparation of medium

[0060] The medium used for sample isolation and strain screening was MRS solid medium, which was purchased from Beijing Luqiao Company. The composition of MRS solid medium is shown in Table 1; the "20g agar" in Table 1 was canceled to obtain MRS liquid medium.

[0061] Use deionized sterile water as a solvent, adjust the pH of the dissolved medium to 6.2-6.4, sterilize with a pressure cooker at 121°C for 15 minutes, pour the unsolidified MRS solid medium into a sterile culture plate at about 55°C, Let stand until the med...

Embodiment 2

[0077] Embodiment 2, co-cultivation of Lactobacillus paracasei ZJUIDS03 inhibits Helicobacter pylori ATCCA43504

[0078] 1. Culture of Helicobacter pylori

[0079] The glycerol tube containing Helicobacter pylori ATCCA43504 was taken out from -80°C, and spread on Columbia blood agar medium containing 5% (v / v) sheep blood for activation culture. The culture conditions were microaerobic environment, 37°C , cultivated for 72h-96h.

[0080] After being activated for one generation in a solid medium (Columbia blood agar medium containing 5% sheep blood), pick a single colony and continue to streak for two generations. Pick a single colony after purification and culture and insert it into the Helicobacter pylori liquid medium , the liquid culture condition is microaerophilic environment, 37°C, and the time is 48h-72h. 8000rmp / min, centrifuge at 4°C for 15min to collect supernatant and precipitate respectively. Wash the pellet twice with clean Helicobacter pylori liquid medium, an...

Embodiment 3

[0101] Embodiment 3, Lactobacillus paracasei ZJUIDS03 inhibit the growth of Helicobacter pylori ATCCA43504

[0102] 1. Culture of Helicobacter pylori

[0103] With the step 1 of embodiment 2.

[0104] 2. Culture of lactic acid bacteria

[0105] Wash the lactic acid bacteria pellet twice with high-temperature sterilized PBS, and resuspend to make the concentration of viable bacteria reach 10 11 CFU / mL; the rest are equal to step 2 of Example 2.

[0106] 3. Growth Inhibition Experiment

[0107] 100 μl of Helicobacter pylori bacteria liquid (10 9 CFU / mL) was applied to a flat plate (about 20ml) of Columbia blood agar medium containing 5% sheep blood without antibiotics and punched, and any of the following was added respectively to form different experimental groups:

[0108] 100 μl of lactic acid bacteria suspension to be tested, 100 μl of lactic acid bacteria supernatant to be tested; 100 μl of positive control (0.025% metronidazole solution); negative control (100 μl of P...

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Abstract

The invention discloses lactobacillus paracasei ZJUIDS03 for antagonizing helicobacter pylori, which is lactobacillus Paracasei, and the preservation number of the lactobacillus paracasei ZJUIDS03 isCGMCC NO. 19857. The compound can be used for preparing a urease activity inhibitor and a helicobacter pylori inhibitor, i.e., can be used for preparing medicines, functional products and health-careproducts capable of preventing stomach diseases caused by helicobacter pylori infection.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a newly screened microorganism, in particular to a lactic acid bacterium having an antagonistic effect on Helicobacter pylori and a bacterium fermentation product having the same inhibitory effect. Background technique [0002] Due to China's large population density and its unique eating habits, the amount of Helicobacter pylori infection in China is the highest in the world. Helicobacter pylori is highly contagious and can be further spread through droplets, tableware contact and kissing. Helicobacter pylori can colonize the stomach and induce varying degrees of gastric ulcer, antral gastritis, gastric cancer and duodenal ulcer. For people with strong immunity, regular work and rest, and a healthy diet, even if there is Helicobacter pylori in the body, it will not cause further damage to the stomach, but for people with weak immunity, severe gastric damage can be induced after infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L2/38A23L13/40A23L33/135A61K35/747A61P31/04A61P1/04A61P1/12A61P39/02C12R1/225
CPCC12N1/20A23L33/135A23L2/382A23L13/45A61K35/747A61P31/04A61P1/04A61P1/12A61P39/02A23V2002/00C12R2001/225C12N1/205A23V2400/165A23V2200/30
Inventor 任大喜梁竟一刘建新
Owner 思我特(杭州)食品科技有限公司
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