Product capable of regulating CYP1A1 gene expression
A gene expression and product technology, applied in the field of microorganisms, can solve the problems of increased expression of CYP1A1 gene and difficult realization, achieve high application prospects, and improve the effect of human intestinal health
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Embodiment 1
[0046] Example 1: Effect of Lactobacillus plantarum CCFM8661 on CYP1A1 gene expression
[0047] Specific steps are as follows:
[0048] 1. Experimental method
[0049] Take 32 male BALB / C mice of SPF grade 8 weeks old, and randomly divide them into 4 groups, 8 mice in each group. The 4 groups are: vehicle control group (Control), model group (Modle), CCFM382 intervention group and CCFM8661 intervention group Group. All mice were kept in a standardized laboratory at 25±2°C, relative humidity 50±5%, 12h light / 12h dark, and the experiment started after one week of adaptive feeding.
[0050] The experiment lasted for 35 days: During the experiment, mice in the carrier control group were orally administered corn oil at a dose of 50 mg / kg.bw per day, and skimmed milk powder solution with a concentration of 130 g / L per day at a dose of 0.2 mL / mouse; the model The mice in the group were gavaged with benzopyrene solution every day according to the dose of 50mg / kg.bw (the benzopyrene...
Embodiment 2
[0059] Example 2: Application of Lactobacillus plantarum CCFM8661
[0060] Specific steps are as follows:
[0061] Lactobacillus plantarum CCFM8661 can be used to prepare bacterial powder. The specific preparation process of bacterial powder is as follows:
[0062] Lactobacillus plantarum CCFM8661 was streaked on the MRS solid medium, cultured at 37°C for 48 hours, and a single colony was obtained; a single colony was picked and inoculated in MRS liquid medium, and activated at 37°C for 18 hours, and the activation continued for two generations. Obtain the activated solution; inoculate the activated solution in the culture medium with an inoculum size of 2% (v / v), cultivate it at 37°C for 18 hours to obtain the bacterial solution; centrifuge the bacterial solution at 5000g for 15 minutes to obtain the bacterial sludge; Wash with pH 7.2 phosphate buffer for 3 times and resuspend with protective agent to a concentration of 1×10 10 CFU / mL to obtain a bacterial suspension; pre-c...
Embodiment 3
[0065] Example 3: Application of Lactobacillus plantarum CCFM8661
[0066] Specific steps are as follows:
[0067] Lactobacillus plantarum CCFM8661 can be used to prepare milk, and the specific preparation process of fermented milk is as follows:
[0068] Lactobacillus plantarum CCFM8661 was streaked on the MRS solid medium, cultured at 37°C for 48 hours, and a single colony was obtained; a single colony was picked and inoculated in MRS liquid medium, and activated at 37°C for 18 hours, and the activation continued for two generations. Obtain the activated solution; inoculate the activated solution in the culture medium with an inoculum size of 2% (v / v), cultivate it at 37°C for 18 hours to obtain the bacterial solution; centrifuge the bacterial solution at 5000g for 15 minutes to obtain the bacterial sludge; Wash with pH 7.2 phosphate buffer for 3 times and resuspend with protective agent to a concentration of 1×10 10 CFU / mL to obtain a bacterial suspension; pre-cultivate t...
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