Technical method for producing aspergillus oryzae from distiller's grains

A technology of distiller's grains and Aspergillus oryzae, which is applied in the field of production of Aspergillus oryzae, can solve the problems of polluting the surrounding environment, easy corruption of distiller's grains, waste of resources, etc., and achieves the effects of easy absorption, strong adaptability and reproductive ability, and avoiding inhibition

Active Publication Date: 2020-11-10
四川润格生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If it is not processed in time, the distiller's grains are prone to corruption, which not only causes a large waste of resources, but also seriously pollutes the surrounding environment

Method used

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  • Technical method for producing aspergillus oryzae from distiller's grains
  • Technical method for producing aspergillus oryzae from distiller's grains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Take Aspergillus oryzae preserved on a slant (purchased from the General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation number: CGMCC No.13575) and streak it on the wort plate for culture, pick 3-5 typical single colonies and continuously Purify, subculture and rejuvenate for 3 times, and select the best single colony of Aspergillus oryzae as the first-class seed liquid strain;

[0038] (2) aseptically pick and screen a single colony of Aspergillus oryzae and inoculate in 5ml glucose 4%, yeast extract 0.2%, NaNO 3 0.3%, KCl 0.05%, balance water, pH 6.8 liquid culture solution, 28°C, 180rpm shaker culture for 16h to obtain the first-grade seed solution;

[0039] (3) Draw 1ml of the first-grade seed liquid and spread it on the wort plate, and continue to cultivate at 28°C. After 5 days, a large number of spores grow on the plate, gently rinse with spore lotion, filter, collect the filtrate, centrifuge at 5000rpm for 10...

Embodiment 2

[0047] Same as in Example 1, the difference is that in step (4) step ① sieves the fresh distiller's grains out of the rice husk and puts it in water, adjusts the pH value to 8, soaks it at 80°C for 24 hours, then dries it and pulverizes it through a 40-mesh sieve to obtain white distiller's grains. Distiller's grain powder and cornstarch are mixed and extruded in a ratio of 8:2.

Embodiment 3

[0049] The same as in Example 1, the difference is that step ① is not crushed by blasting.

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Abstract

The invention discloses a technical method for producing aspergillus oryzae from distillers' grains. The technical method comprises the steps: aspergillus oryzae stored on an inclined plane is taken to be streaked onto a wort plate for culture, and optimal aspergillus oryzae single colonies are selected; the screened aspergillus oryzae single colonies are selected through sterile operation to be inoculated into a liquid culture solution, and shaking culture is conducted to obtain a first-stage seed solution; the first-stage seed solution is sucked to coat a plate, after growing on the plate, spores are gently washed with spore washing liquor and filtered, filtrate is collected, and centrifuging is conducted to discard supernatant to obtain aspergillus oryzae spores; the prepared aspergillus oryzae spores are inoculated into a solid-state fermentation culture medium containing the pretreated distiller's grains, and fermentation culture is conducted; and after the completion of fermentation, a fermentation product is dried and crushed to obtain an aspergillus oryzae solid-state fermentation product. The survival rate of the aspergillus oryzae still can be guaranteed in the storage process, the storage life of strain products is prolonged, and the reproductive capacity, neutral protease activity and raw material decomposition capacity of aspergillus oryzae strains are not reducedafter the aspergillus oryzae strains are stored at the low temperature for 12 months.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a technical method for producing Aspergillus oryzae by using distiller's grains. Background technique [0002] Aspergillus oryzae is a kind of Aspergillus, and its most important function is to generate proteolytic enzymes, that is, to decompose macromolecules in raw materials into small molecular substances to form soy sauce flavor substances, flavor substances or their precursors. These include proteases, peptidases, and protein-related enzymes that break down proteins, as well as enzymes that break down carbohydrates and others. The most important of these enzymes are proteases, peptidases and related enzymes, because the activity of proteases and peptidases is improved, the rate of proteolysis can be improved, and the brewing cycle can also be shortened. Aspergillus oryzae constitutes the source of aromatic substances. The role of Aspergillus oryzae is not ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/38C12R1/69
CPCC12N1/14C12N1/38Y02P60/87
Inventor彭楠常章兵田建平梁运祥
Owner四川润格生物科技有限公司