Multifunctional hybrid peptide with antibacterial, anti-inflammatory, endotoxin neutralizing and immunomodulatory activities as well as preparation method and application thereof

Abstract

The invention relates to the technical field of protein engineering, in particular to a multifunctional hybrid peptide with antibacterial, anti-inflammatory, endotoxin neutralizing and immunomodulatory activities as well as a preparation method and application thereof. The hybrid peptide provided by the invention comprises a peptide I and a peptide II fused with the peptide I, wherein an amino acid sequence of the peptide I is as shown in SEQ ID NO.1, and an amino acid sequence of the peptide II is as shown in 19th-29th sites of SEQ ID NO.2. The hybrid peptide has antibacterial, endotoxin neutralizing, immunoregulation and anti-inflammatory functions, has the advantages of low cytotoxicity, high safety, convenience in preparation and low cost, can be used as an ideal antibacterial agent, endotoxin neutralizing agent, immunoregulator and anti-inflammatory agent, is widely applied to the fields of medicines, foods, health care, feeds, nutrition and the like of human beings and animals, and has good application potential and value.

Description

technical field [0001] The invention relates to the technical field of protein engineering, in particular to a hybrid peptide with antibacterial, anti-inflammatory, endotoxin-neutralizing and immune-modulating activities, its preparation method and application. Background technique [0002] Bacterial infection is an infectious disease caused by pathogenic bacteria or conditional pathogenic bacteria invading the animal body to grow and reproduce, producing toxins (such as endotoxin) and other metabolites, which can induce inflammation and oxidative damage, etc., and damage the immune system in severe cases. even lead to death. When animals are young, weak, sick, stressed, or infected with pathogens, their immunity is often reduced, and then secondary infections (bacterial infections, etc.) occur, causing inflammatory reactions. For the treatment of infection and inflammation, the traditional control strategy is to use antibiotics and chemical drug agents. The continuous use...

AI Technical Summary

Problems solved by technology

[0006] However, natural bioactive peptides also have some defects, mainly as follows: (1) Some natural bioactive peptides have relatively low biological activity and single function, such as the problem of weak antibacterial activity of pig lactoferrin peptide ; and although cecropin A has good antibacterial efficacy, its anti-inflammatory ability is relatively weak; (2) Some natural bioactive peptides have high cytotoxicity, which can cause serious damage to the body while inhibiting and killing pathogenic microorganisms (such as melittin and LL-37)

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