Method for evaluating water body health risk based on resistance gene and virulence factor gene

A technology for virulence factors and water health, applied in the fields of genomics, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of reducing the therapeutic effect of antibiotics, and achieve high reliability and accurate detection results

Pending Publication Date: 2020-11-17
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Potential drug-resistant pathogenic bacteria (PARB) that carry both ARGs and VFGs in their genomes have potential pathogenic risks and can reduce the therapeutic effect of antibiotics to a certain extent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating water body health risk based on resistance gene and virulence factor gene
  • Method for evaluating water body health risk based on resistance gene and virulence factor gene
  • Method for evaluating water body health risk based on resistance gene and virulence factor gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described below with reference to specific examples, but those skilled in the art should understand that the present invention is not limited to these specific examples.

[0025] The methods in the following examples, unless otherwise specified, are conventional methods, and the reagents used therein, unless otherwise specified, are conventional commercially available reagents.

[0026] 1 Sample collection and data analysis methods

[0027] 1.1 Sample collection

[0028] The Tonghui River, the receiving river of Beijing Gaobeidian Sewage Treatment Plant, was selected as the research object. The upgrade of Gaobeidian Sewage Treatment Plant was completed in April 2017. The Tonghui River was sampled in September 2018) and after the upgrade (September 2018, December 2018, March 2019). At the same time, Qinghe, the receiving river of Qinghe Sewage Treatment Plant (upgraded and renovated in December 2013), was selected as a control and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for evaluating water body health risk based on a resistance gene and a virulence factor gene. The method is based on metagenome sequencing analysis, and comprises the following steps of: obtaining a microbial genome sketch by assembling and binning sequencing data, identifying potential drug-resistant pathogenic bacteria (PARB) from the microbial genome sketch, evaluating the abundance, diversity and risk level of the potential PARB in the sample, and evaluating the quantity and types of ARGs and VFGs contained in the PARB, thereby determining the quantity and health risk level of the potential PARB in the environment. The method of the invention is based on metagenome sequencing, potential PARB can be recognized on genome level, a high degree of credibilityis shown, the limitations that a traditional pure culture method consumes time and is prone to being affected by culture conditions are avoided, and the method can be widely applied to water body health risk evaluation.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a method for evaluating water body health risks based on resistance genes and virulence factor genes. Background technique [0002] Affected by human activities, the water quality of rivers and lakes has gradually deteriorated, and the health risks of polluted water bodies have attracted more and more attention. At present, an important content of health risk assessment in water environment is the assessment of the distribution of pathogenic bacteria in microorganisms. The methods commonly used to detect pathogenic bacteria include pure culture method and PCR-based molecular detection. The traditional pure culture method is time-consuming and easily affected by culture conditions, making it difficult to apply to large-scale ecological surveys; PCR-based detection methods are severely limited by the specificity of primers for amplifying the target gene, and i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6869C12Q1/04G16B20/00G16B30/10
CPCC12Q1/689C12Q1/6869G16B20/00G16B30/10
Inventor 柏耀辉王巧娟梁金松廖恺玲俐曲久辉
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap