Method for evaluating water body health risk based on resistance gene and virulence factor gene

Abstract

The invention provides a method for evaluating water body health risk based on a resistance gene and a virulence factor gene. The method is based on metagenome sequencing analysis, and comprises the following steps of: obtaining a microbial genome sketch by assembling and binning sequencing data, identifying potential drug-resistant pathogenic bacteria (PARB) from the microbial genome sketch, evaluating the abundance, diversity and risk level of the potential PARB in the sample, and evaluating the quantity and types of ARGs and VFGs contained in the PARB, thereby determining the quantity and health risk level of the potential PARB in the environment. The method of the invention is based on metagenome sequencing, potential PARB can be recognized on genome level, a high degree of credibilityis shown, the limitations that a traditional pure culture method consumes time and is prone to being affected by culture conditions are avoided, and the method can be widely applied to water body health risk evaluation.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a method for evaluating water body health risks based on resistance genes and virulence factor genes. Background technique [0002] Affected by human activities, the water quality of rivers and lakes has gradually deteriorated, and the health risks of polluted water bodies have attracted more and more attention. At present, an important content of health risk assessment in water environment is the assessment of the distribution of pathogenic bacteria in microorganisms. The methods commonly used to detect pathogenic bacteria include pure culture method and PCR-based molecular detection. The traditional pure culture method is time-consuming and easily affected by culture conditions, making it difficult to apply to large-scale ecological surveys; PCR-based detection methods are severely limited by the specificity of primers for amplifying the target gene, and i...

AI Technical Summary

Problems solved by technology

Potential drug-resistant pathogenic bacteria (PARB) that carry both ARGs and VFGs in their genomes have potential pathogenic risks and can reduce the therapeutic effect of antibiotics to a certain extent

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