Improved mouse bone marrow tissue section H.E staining method
A technology of bone marrow tissue and staining method, applied in the field of detection, can solve the problems of poor cell staining effect, unfavorable tissue sectioning, difficult to grasp the length of ethanol cleaning, shrinkage and hardening of bone tissue, etc., to achieve fast and efficient staining standards, enhanced staining effect, Reduce the effect of concentration gradients
Pending Publication Date: 2020-12-11
FOSHAN UNIVERSITY
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Problems solved by technology
[0009] However, due to the hematoxylin soaking and staining time and the running water washing time after staining, the eosin soaking and staining time and the ethanol washing time after staining are difficult to grasp, too long or too short will have a great impact on cell staining, and poor cell staining effect is not conducive to tissue Observation of slices, and high concentration of alcohol will shrink and harden the decalcified and softened bone tissue
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Embodiment 1
[0025] The chicken bursa paraffin samples were stained by modified H.E staining method. After processing the sample slices, stain according to the following steps:
[0026]
[0027]
[0028] (19) Neutral gum sealing: use a glass rod dipped in neutral gum, drop it on the edge of the tissue on the slide, pick up the edge of the slide with tweezers, and gently cover the tissue with the cover glass, so that the neutral gum is on the cover glass. Diffusion under the film freely, just cover the tissue.
[0029] made as figure 1 The stained slides shown, from figure 1 It can be seen that the staining degree of the tissue is deep, the contrast is obvious, and the staining effect is good.
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The invention belongs to the technical field of detection methods, and discloses an improved mouse bone marrow tissue section H.E staining method. The method comprises the following steps: properly increasing duration of a xylene dewaxing transparency step in an early stage, and fully dissolving paraffin so that a staining solution fully enters tissues during staining; meanwhile, reducing a concentration gradient of alcohol and soaking dehydration duration of high-concentration alcohol, and effectively preventing a situation that softened bone tissue shrinks and hardens, and section quality isaffected; enabling the sections to be subjected to bluing by utilizing a weakly alkaline solution 2% sodium bicarbonate so that bluing efficiency and effect are improved, and cost is reduced; and using the distilled water for washing after blue returning so that a staining effect of an eosin solution is improved. According to the improved mouse bone marrow tissue section H.E staining method, thestaining time of the tissue section is reasonably controlled so that the soaking time of each step is more reasonable, the staining effect of the bone marrow tissue section is improved, a rapid and efficient tissue section staining standard is achieved, and an application prospect is wide.
Description
technical field [0001] The invention belongs to the technical field of detection methods, and in particular relates to an improved H.E. staining method for mouse bone marrow tissue sections. Background technique [0002] Hematoxylin-eosin staining (H.Ematoxylin-eosin staining), referred to as H.E staining, is one of the commonly used staining methods in paraffin section technology. The hematoxylin staining solution is alkaline, which mainly makes the chromatin in the nucleus and the nucleic acid in the cytoplasm stain purple blue; eosin is an acid dye, which mainly makes the components in the cytoplasm and extracellular matrix red. [0003] In histopathological observation, it is often necessary to make pathological sections of the bone marrow of mice in order to observe the effects of diseases or drugs on bone marrow tissue structure, bone marrow cell morphology and quantity; H.E staining is the most widely used in tissue sections. The paraffin sections are stained with al...
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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 邓桦张伟伦李樵锋吴春琼梁浩钊杨鸿
Owner FOSHAN UNIVERSITY
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