Method for preparing beta-hyodeoxycholic acid by microbial transformation

A technology for transformation of hyodeoxycholic acid and microorganisms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as inability to achieve large-scale production, large environmental pollution, and complicated processes, and promote development and utilization , low cost, simple and convenient preparation process

Inactive Publication Date: 2020-12-18
CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for the preparation of β-hyodeoxycholic acid by microbial transformation, to solve the problems raised in the above background. At present, the preparation of β-hyodeoxycholic acid is mainly synthesized by chemical method, which has complicated process and great environmental pollution. , the output is low, and the problem that large-scale production cannot be realized

Method used

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  • Method for preparing beta-hyodeoxycholic acid by microbial transformation
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  • Method for preparing beta-hyodeoxycholic acid by microbial transformation

Examples

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Embodiment 1

[0036] A method for preparing β-hyodeoxycholic acid by microbial transformation, the specific preparation process is as follows:

[0037] (1) Preparation of spores and seeds

[0038] Take 200g of peeled potatoes, add 200ml of deionized water and boil for 25min; filter while hot with sterilized double-layer gauze, discard the filter residue, and keep the filtrate; Stir with a glass rod until it is completely dissolved, add deionized water to make up to 1000ml; divide the prepared medium into a 500mL Erlenmeyer flask, block the mouth of the bottle with a cotton plug, and wrap a layer of kraft paper outside the cotton plug. Indicate the name of the medium, the date of preparation and the person who prepared it; sterilize in the sterilizer at 115°C for 30 minutes, and after the medium is cooled to about 50°C, pour it into a plate (about 15ml per plate) aseptically, and place it flat at room temperature. After coagulation, a PDA plate was prepared; the mycelium of Bacillus sphaeri...

Embodiment 2

[0053] A method for preparing β-hyodeoxycholic acid by microbial transformation, the specific preparation process is as follows:

[0054] (1) Preparation of spores and seeds

[0055] Take 200g of peeled potatoes, add 200ml of deionized water and boil for 25min; filter while hot with sterilized double-layer gauze, discard the filter residue, and keep the filtrate; Stir with a glass rod until it is completely dissolved, add deionized water to make up to 1000ml; divide the prepared medium into a 500mL Erlenmeyer flask, block the mouth of the bottle with a cotton plug, and wrap a layer of kraft paper outside the cotton plug. Indicate the name of the medium, the date of preparation and the person who prepared it; sterilize in the sterilizer at 115°C for 30 minutes, and after the medium is cooled to about 50°C, pour it into a plate (about 15ml per plate) aseptically, and place it flat at room temperature. After coagulation, a PDA plate was prepared; the mycelium of Bacillus sphaeri...

Embodiment 3

[0063] A method for preparing β-hyodeoxycholic acid by microbial transformation, the specific preparation process is as follows:

[0064] (1) Preparation of spores and seeds:

[0065] Take 200g of peeled potatoes, add 200ml of deionized water and boil for 25min; filter while hot with sterilized double-layer gauze, discard the filter residue, and keep the filtrate; Stir with a glass rod until it is completely dissolved, add deionized water to make up to 1000ml; divide the prepared medium into a 500mL Erlenmeyer flask, block the mouth of the bottle with a cotton plug, and wrap a layer of kraft paper outside the cotton plug. Indicate the name of the medium, the date of preparation and the person who prepared it; sterilize in the sterilizer at 115°C for 30 minutes, and after the medium is cooled to about 50°C, pour it into a plate (about 15ml per plate) aseptically, and place it flat at room temperature. After coagulation, a PDA plate was prepared; the mycelium of Bacillus sphaer...

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Abstract

The invention discloses a method for preparing beta-hyodeoxycholic acid by microbial transformation. According to the method, the hydroxyl group at the 3-position of hyodeoxycholic acid is transformedfrom the alpha-position to the beta-position by microbial transformation. The method comprises the following steps: preparing spores and seeds; performing microbial transformation on alpha-hyodeoxycholic acid; and performing separation and purification. The method disclosed by the invention is simple to operate, the alpha-hyodeoxycholic acid with rich resources and low price is taken as a raw material, and the beta-hyodeoxycholic acid is obtained by biological transformation, so that industrial production of the beta-hyodeoxycholic acid is realized, and development and utilization of the beta-hyodeoxycholic acid are promoted.

Description

technical field [0001] The invention belongs to the technical field of microbial transformation, and in particular relates to a method for preparing β-hyodeoxycholic acid by microbial transformation, which uses microorganisms to convert the hydroxyl group at the 3-position of hyodeoxycholic acid from an α-position to a β-position. Background technique [0002] α-hyodeoxycholic acid (3α, 6α-dihydroxycholic acid, HDCA) is extracted and processed from pig bile, and has the effects of lowering blood fat, expectorant and antitussive. α-Hyodeoxycholic acid has a certain inhibitory effect on Bacillus pertussis, Bacillus diphtheriae, Staphylococcus aureus, etc. It can be used as an anti-inflammatory drug to treat chronic bronchitis, viral upper respiratory tract inflammation in children, etc. At present, the extraction and preparation process of α-hyodeoxycholic acid is relatively mature, and large-scale processing and production have been realized in industry. Based on its own char...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P41/00C12P33/00C12N1/20C12R1/07
CPCC12N1/20C12P33/00C12P41/001
Inventor 邓家国魏红霞李国军
Owner CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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