A magnetically controlled high-throughput electrochemical sensor and its preparation method and application

An electrochemical and sensor technology, applied in the field of magnetically controlled high-throughput electrochemical sensors and preparation, can solve the problems of cumbersome pretreatment steps, poor repeatability, and expensive equipment

Active Publication Date: 2021-07-02
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of cumbersome sample pretreatment steps, expensive instruments and equipment, and the need for professional and technical personnel to operate in the traditional oxytetracycline detection method, as well as the difficulty in modifying the detection electrode of the traditional molecularly imprinted electrochemical sensing, poor repeatability, and inability to achieve high Flux detection and other technical issues

Method used

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  • A magnetically controlled high-throughput electrochemical sensor and its preparation method and application
  • A magnetically controlled high-throughput electrochemical sensor and its preparation method and application
  • A magnetically controlled high-throughput electrochemical sensor and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A method for preparing a magnetic MOFs surface molecularly imprinted material, comprising the steps of:

[0038] Step 1, polydopamine functionalized magnetic nanoparticles (Fe 3 o 4 @PDA) material preparation: take 20mg of dopamine hydrochloride and add it to 30mL of 10mmol / L Tris buffer solution, after ultrasonic for 5min, ultrasonic power 100W, 5mg Fe 3 o 4 Evenly dispersed in the above solution, stirred at room temperature for 12 hours, after the reaction, the black product was magnetically separated, washed with ultrapure water, and then placed in a vacuum oven, dried at 50°C for 2 hours to obtain Fe 3 o 4 @PDAMaterial;

[0039] Step 2, MOFs functionalized Fe 3 o 4 Preparation of @PDA: the Fe prepared in step 1 3 o 4 @PDA, 70mg of anhydrous copper acetate, 60mg of 1,3,5-benzenetricarboxylic acid were added to 30mL of absolute ethanol in sequence, and heated at 70°C for 30min. After the reaction, the product was magnetically separated and washed with ultrapur...

Embodiment 2

[0042] A method for preparing a magnetic MOFs surface molecularly imprinted material, comprising the steps of:

[0043] Step 1, polydopamine functionalized magnetic nanoparticles (Fe 3 o 4 @PDA) Material preparation: Take 100 mg of dopamine hydrochloride and add it to 50 mL of 10 mmol / L Tris buffer solution. After ultrasonication for 10 min, the ultrasonic power is 100 W, 3 o 4 Evenly dispersed in the above solution, stirred at room temperature for 20h, after the reaction, the black product was magnetically separated, and washed with ultrapure water, and then the black product was placed in a vacuum drying oven at 50°C for 10h to obtain Fe 3 o 4 @PDAMaterial;

[0044] Step 2, MOFs functionalized Fe 3 o 4 Preparation of @PDA: the Fe prepared in step 1 3 o 4 Add @PDA, 90mg of anhydrous copper acetate, and 100mg of 1,3,5-benzenetricarboxylic acid to 50mL of absolute ethanol in sequence, and heat at 70°C for 30min. After the reaction, the product is magnetically separated ...

Embodiment 3

[0047] A method for preparing a magnetic MOFs surface molecularly imprinted material, comprising the steps of:

[0048] Step 1, polydopamine functionalized magnetic nanoparticles (Fe 3 o 4 @PDA) Material preparation: Add 40mg of dopamine hydrochloride to 40mL of 10mmol / L Tris buffer solution, after ultrasonication for 8min, ultrasonic power 100W, 8mg Fe 3 o 4 Evenly dispersed in the above solution, stirred at room temperature for 15 hours, after the reaction, the black product was magnetically separated and washed with ultrapure water, then the black product was placed in a vacuum oven and dried at 50°C for 5 hours to obtain Fe 3 o 4 @PDAMaterial;

[0049] Step 2, MOFs functionalized Fe 3 o 4 Preparation of @PDA: the Fe prepared in step 1 3 o 4 Add @PDA, 80mg of anhydrous copper acetate, and 70mg of 1,3,5-benzenetricarboxylic acid to 40mL of absolute ethanol in sequence, and heat at 70°C for 30min. After the reaction, the product is magnetically separated and washed wi...

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Abstract

The invention belongs to the technical field of rapid detection of food safety, and in particular relates to a magnetically controlled high-throughput electrochemical sensor based on a magnetic MOFs surface molecular imprinting material, a preparation method and an application. The present invention uses magnetic MOFs as a carrier to directly perform molecular imprinting on its surface, and then combines the molecular imprinting polymer on the surface of the magnetic MOF with an electrochemical sensor, so that the constructed magnetically controlled high-throughput electrochemical sensor not only has the ability to identify target compounds , specificity of extraction and separation, simple steps, high efficiency, etc., and also has the advantages of high sensitivity of electrochemical sensors. In addition, the magnetically controlled high-throughput electrochemical sensor is applied to the detection of oxytetracycline, and the method is simple and easy to operate. The invention has the advantages of good selectivity, high sensitivity, good reproducibility and the like, and is suitable for the field of rapid detection of food safety.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of food safety, and in particular relates to a magnetically controlled high-throughput electrochemical sensor based on a magnetic MOFs surface molecular imprinting material, a preparation method and an application. Background technique [0002] Oxytetracycline (OTC) belongs to the antibiotics of tetracyclines, which not only has bactericidal and antibacterial effects, but also can promote the growth of animals, so it is often used in animal breeding. And use is unreasonable, will cause its residue in animal food, and people's long-term edible food that contains oxytetracycline residue will be harmful to the health of human body. At present, there have been a large number of reports on the analysis and detection methods of oxytetracycline residues in food, but the instrument analysis method based on chromatographic technology uses expensive instruments, complicated operation, and the method...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/48
CPCG01N27/26G01N27/308G01N27/48
Inventor杨钰昆常媛媛王小敏白宝清张锦华郭彩霞尉立刚范三红
OwnerSHANXI UNIV