Magnetic microsphere with molecular tag sequence, and preparation method for magnetic microsphere

A technology of magnetic microspheres and molecular tags, which is applied in the field of molecular biology, can solve problems affecting the stability and accuracy of single-cell sequencing results, high cost of single-cell sequencing, and unstable batch results, so as to achieve convenient source of raw materials, The effect of low cost and small batch variance
CN112251504APending Publication Date: 2021-01-22SINGLERON NANJING BIOTECHNOLOGIES LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SINGLERON NANJING BIOTECHNOLOGIES LTD
Publication Date
2021-01-22

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Abstract

The invention belongs to the field of the molecular biology, and particularly relates to a magnetic microsphere with a molecular tag sequence, and a preparation method for the magnetic microsphere. Amagnetic bead is taken as a molecular tag base, and a chemical bond and enzyme linking method is used for linking three-segment oligo to the magnetic bed in sequence so as to synthesize the magnetic microsphere with a unique oligo sequence. The magnetic microsphere prepared by the preparation method disclosed by the invention has high stability and accuracy. Meanwhile, the preparation technology of the magnetic microsphere is simple, time consumption is small, raw material sources are convenient, the cost is low, and the unicellular sequencing cost is greatly lowered. The oligo adopted by themagnetic microsphere is a known sequence, the three-segment oligo can be combined to form millions of molecular tags, batch differences are small, the stability and the accuracy of a unicellular sequencing result are obviously improved, and the magnetic microsphere can be widely applied to medical research, bioresearch and the like.
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Description

technical field

[0001] The invention belongs to the field of molecular biology, and in particular relates to a magnetic microsphere with a molecular tag sequence and a preparation method thereof. Background technique

[0002] Single-cell transcriptome sequencing (single-cell RNA-sequencing, scRNA-seq) is a new technology that has emerged in recent years. It can obtain the expression profile of the whole transcriptome from the single cell level, perform high-throughput sequencing after amplification, and efficiently Detecting the gene expression level in a single cell has important application value in the development and differentiation of stem cells, diagnosis and treatment of tumors, and the design of targeted drugs.

[0003] The reason why single-cell transcriptome sequencing can sequence hundreds of thousands of cells at the same time is that each cell has a different label. In order to distinguish each mRNA in each cell, each mRNA is also given a unique molecular label...

Claims

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