Application and product of a combined metabolic marker
A metabolic marker and combined technology, applied in biological testing, material inspection products, biological material analysis, etc., can solve the problems of low sensitivity, false negative rate, false positive rate and other problems of liver cancer, and achieve better diagnostic efficiency, The effect of easy operation and simplified detection method
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Embodiment 1
[0025] Volunteers were divided into three groups: liver cancer patients, liver cirrhosis patients and healthy volunteers. The peripheral blood samples of the three groups of volunteers were collected from December 2016 to December 2018 in the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province. Peripheral blood from 27 patients with liver cancer, average age 52.78±9.42, male to female ratio 20:7; peripheral blood from 23 patients with liver cirrhosis, average age 47.83±15.10, male to female ratio 20:3; peripheral blood from 30 healthy volunteers. All volunteers obtained informed consent. The results of liver cancer in the liver cancer group were confirmed by histopathological results, and the results of liver cirrhosis in the liver cirrhosis group were confirmed by magnetic resonance imaging or B-ultrasound. The study protocol was conducted in accordance with the ethical guidelines of the 1975 Declaration of Helsinki.
[0026] After collecting th...
Embodiment 2
[0037] Liver function indicators aspartate (AST) and alanine aminotransferase (ALT) were measured by automatic biochemical analyzer, AFP was measured by electrochemiluminescence immunoassay with MICROLAB FAME microplate reader, and Spearman correlation analysis was used to compare serum 2-hydroxy-butyric acid, The correlation between the expression level of 2E-eicosenoic acid and AFP, classic liver function indexes (AST, ALT). The result is as Figure 2-3 shown. Depend on figure 2 It can be seen that we found that there is a positive correlation between the expression of 2-hydroxy-butyric acid and 2E-eicosenoic acid and AFP, and the correlation coefficients R are 0.597 (p=0.001) and 0.43 (p=0.028), respectively. Depend on image 3It can be seen that there is a positive correlation between 2-hydroxy-butyric acid and liver function index alanine aminotransferase ALT (R=0.412, p=0.036), and there is a positive correlation between 2E-eicosenoic acid and liver function index as...
Embodiment 3
[0039] Receiver operating characteristic (ROC) curve analysis was constructed to obtain the area under the curve (AUC) value of the ROC curve, and the optimal cut-off value for the diagnosis of liver cancer was determined according to the point corresponding to the maximum Youden index, and then obtained The sensitivity and specificity of diagnosing liver cancer, comparing the diagnostic efficiency of 2-hydroxy-butyric acid combined with 2E-eicosenoic acid and AFP in serum for liver cancer. The result is as Figure 4-6 shown.
[0040] Depend on Figure 4 and Figure 5 It can be seen that the area under the ROC curve (0.987) of 2-hydroxy-butyric acid combined with 2E-eicosenoic acid to distinguish liver cancer and cirrhosis is higher than that of AFP (0.904), so 2-hydroxy-butyric acid combined with 2E - Eicosenoic acid is more effective than AFP in distinguishing liver cancer and liver cirrhosis, and is determined by Figure 5 It can be seen that the combined diagnostic per...
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