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Chemical mutagenesis method for increasing mutation range of dwarf bananas through induction

A technology of chemical mutagenesis and mutation range, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., and can solve problems such as small development space, small variation range, and incomplete stress resistance of varieties

Active Publication Date: 2021-02-02
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the development space of new banana varieties cultivated by hybridization is small, the stress resistance of the varieties is not comprehensive, and the variation range is small, it is difficult to produce new gene mutants through natural mutation, and the current resistance to mutagens of bananas is limited. Different, although a single mutagenesis method has a high mutation frequency in a certain aspect, the mutation rate of the overall trait is still low, resulting in fewer mutation types and low efficiency of mutation breeding

Method used

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  • Chemical mutagenesis method for increasing mutation range of dwarf bananas through induction
  • Chemical mutagenesis method for increasing mutation range of dwarf bananas through induction

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Embodiment 1

[0022] A chemical mutagenesis method for inducing and increasing the range of mutations in bananas: comprising the following steps:

[0023] S1, the preparation of proliferation medium: MS medium, 6-benzylaminopurine (BAP) 1.0mg / L, adenine 0.2mg / L, agarose 2.0g / L, sucrose 20g / L, sucrose pH is 4.0;

[0024] S2. Mutagenesis treatment of bananas: Use a scalpel to cut a single independent bud, put it in a triangular flask equipped with a lithium chloride mutagen, shake and soak it in a rotary shaker at 138rpm and 20°C for 4h, and then Place it in a light incubator, set the light intensity to 1000lx, and then carry out variable temperature treatment. The initial temperature is 15°C for 1h, and the temperature is adjusted to 22°C for 1h; the lithium chloride mutagen is lithium chloride solution in Add Tween-80 and banana peel polysaccharide solution to sterilized water, filter and sterilize to obtain, the volume ratio of the lithium chloride solution, sterilized water, Tween-80 and ...

Embodiment 2

[0028] A chemical mutagenesis method for inducing and increasing the range of mutations in bananas: comprising the following steps:

[0029] S1, the preparation of proliferation medium: MS medium, 6-benzylaminopurine (BAP) 5.0mg / L, adenine 1.5mg / L, agarose 4.0g / L, sucrose 40g / L, sucrose pH is 6.0;

[0030] S2. Mutagenesis treatment of bananas: Use a scalpel to cut a single independent bud, put it in a triangular flask equipped with a lithium chloride mutagen, shake and soak it at 200rpm at 30°C for 24 hours in a rotary shaker, and then Place it in a light incubator, set the light intensity to 3000lx, and then carry out variable temperature treatment. The initial temperature is 20°C for 3h, and the temperature is adjusted to 30°C for 3h; the lithium chloride mutagen is lithium chloride solution in Add Tween-80 and banana peel polysaccharide solution to sterilized water, filter and sterilize to obtain, the volume ratio of the lithium chloride solution, sterilized water, Tween-80...

Embodiment 3

[0034] A chemical mutagenesis method for inducing and increasing the range of mutations in bananas: comprising the following steps:

[0035] S1, preparation of proliferation medium: MS medium, 6-benzylaminopurine (BAP) 3.0mg / L, adenine 1.0mg / L, agarose 3.0g / L, sucrose 30g / L;

[0036] S2. Mutagenesis treatment of bananas: Use a scalpel to cut a single independent bud, put it in a triangular flask equipped with a lithium chloride mutagen, shake and soak it at 150rpm at 27°C for 14 hours in a rotary shaker, and then Place it in a light incubator, set the light intensity to 2000lx, and then carry out variable temperature treatment. The initial temperature is 18°C ​​for 2h, and the temperature is adjusted to 26°C for 2h; the lithium chloride mutagen is lithium chloride solution in Add Tween-80 and banana peel polysaccharide solution to sterilized water, filter and sterilize to obtain, the volume ratio of the lithium chloride solution, sterilized water, Tween-80 and banana peel poly...

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Abstract

The invention provides a chemical mutagenesis method for increasing the mutation range of dwarf bananas through induction. The chemical mutagenesis method comprises the following steps of preparationof a proliferation culture medium, mutagenesis treatment of the bananas, washing treatment and proliferation culture. According to the chemical mutagenesis method, a lithium chloride solution is adopted to treat and inhibit the growth of seedlings, so that the growth vigor is obviously different, and single plants with special characters appear in form; through mutagenic agent treatment, the mutation frequency is increased, the variation range is expanded, and the mutation frequency can be increased to 8.89% at most and is 1000 times or above higher than the natural mutation frequency throughchemical mutagenic agent treatment; and the mutation types are intensively represented as leaf color mutation, leaf type mutation and plant type mutation, wherein 6.47% of plants are represented to have bright green leaves, 7.11% of the plants are represented to have yellow stripes, 1.96% of the plants are represented to have leaf edge curling, and 4.50% of the plants are represented to have shortleaves, so that the artificial mutation range is wide, and the mutation types are rich.

Description

technical field [0001] The invention relates to the planting field of plant polyploid mutagenesis breeding technology, in particular to a chemical mutagenesis method for inducing and increasing the mutation range of plantain. Background technique [0002] Banana is a typical tropical and subtropical fruit tree, widely distributed in tropical and subtropical regions. my country is one of the main banana producing countries in the world, and the planting areas are distributed in Guangdong, Hainan, Guangxi, Fujian, Yunnan and Taiwan. But at present, the development space of new banana varieties cultivated by hybridization is small, the stress resistance of the varieties is not comprehensive, and the range of variation is small, it is difficult to produce new gene mutants through natural mutation, and the current resistance to mutagens of bananas is limited. Different, although a single mutagenesis method has a high mutation frequency in a certain aspect, the mutation rate of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 李敬阳许竹叶魏卿王安邦李羽佳王笑一许奕
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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