Chemical mutagenesis method for increasing mutation range of dwarf bananas through induction

Abstract

The invention provides a chemical mutagenesis method for increasing the mutation range of dwarf bananas through induction. The chemical mutagenesis method comprises the following steps of preparationof a proliferation culture medium, mutagenesis treatment of the bananas, washing treatment and proliferation culture. According to the chemical mutagenesis method, a lithium chloride solution is adopted to treat and inhibit the growth of seedlings, so that the growth vigor is obviously different, and single plants with special characters appear in form; through mutagenic agent treatment, the mutation frequency is increased, the variation range is expanded, and the mutation frequency can be increased to 8.89% at most and is 1000 times or above higher than the natural mutation frequency throughchemical mutagenic agent treatment; and the mutation types are intensively represented as leaf color mutation, leaf type mutation and plant type mutation, wherein 6.47% of plants are represented to have bright green leaves, 7.11% of the plants are represented to have yellow stripes, 1.96% of the plants are represented to have leaf edge curling, and 4.50% of the plants are represented to have shortleaves, so that the artificial mutation range is wide, and the mutation types are rich.

Description

technical field [0001] The invention relates to the planting field of plant polyploid mutagenesis breeding technology, in particular to a chemical mutagenesis method for inducing and increasing the mutation range of plantain. Background technique [0002] Banana is a typical tropical and subtropical fruit tree, widely distributed in tropical and subtropical regions. my country is one of the main banana producing countries in the world, and the planting areas are distributed in Guangdong, Hainan, Guangxi, Fujian, Yunnan and Taiwan. But at present, the development space of new banana varieties cultivated by hybridization is small, the stress resistance of the varieties is not comprehensive, and the range of variation is small, it is difficult to produce new gene mutants through natural mutation, and the current resistance to mutagens of bananas is limited. Different, although a single mutagenesis method has a high mutation frequency in a certain aspect, the mutation rate of t...

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Problems solved by technology

But at present, the development space of new banana varieties cultivated by hybridization is small, the stress resistance of the varieties is not comprehensive, and the variation range is small, it is difficult to produce new gene mutants through natural mutation, and the current resistance to mutagens of bananas is limited. Different, although a single mutagenesis method has a high mutation frequency in a certain aspect, the mutation rate of the overall trait is still low, resulting in fewer mutation types and low efficiency of mutation breeding

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