Method for establishing Caihong populus regeneration system by taking petioles as explants

A technology of explants and poplar, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve problems such as high differentiation rate, low pollution rate, and difficulty in establishing the first generation of sterile lines

Inactive Publication Date: 2021-02-09
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is very difficult to establish the first-generation sterile line, and the method of regeneration

Method used

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  • Method for establishing Caihong populus regeneration system by taking petioles as explants
  • Method for establishing Caihong populus regeneration system by taking petioles as explants

Examples

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Embodiment 1

[0039] A method for establishing a poplar regeneration system with petioles as explants, comprising:

[0040] 1. Selection and disinfection of explants: At the end of June, select young petioles with a length of 2-3 cm, shake them with detergent for 10 minutes, and rinse them with running water for 30 minutes. Shake with 75% alcohol for 1 minute, then shake with 0.1% mercuric chloride for 12 minutes, wash with sterile water 6 times to remove residual disinfectant. A total of 100 explants were processed;

[0041] 2. Induction of callus: remove the petiole explants after detoxification, cut off 0.5 cm at both ends, inoculate in induction medium MS+6-BA 1 mg / L +NAA 0.4 mg / L for adventitious bud induction, The culture temperature was controlled at 26°C, and cultured for 25 days. The culture conditions were: light time 14h / day, and light intensity 1800lx; after 25 days, the induction rate of explants was 42%, that is, 42 explants grew out of different sizes. callus;

[0042] 3. ...

Embodiment 2

[0046] A method for establishing a poplar regeneration system with petioles as explants, comprising:

[0047] 1. Selection and disinfection of explants: At the end of June, select young petioles with a length of 2-3 cm, shake them with detergent for 10 minutes, and rinse them with running water for 30 minutes. Shake with 75% alcohol for 1 minute, then shake with 0.1% mercuric chloride for 12 minutes, wash with sterile water 6 times to remove residual disinfectant. A total of 100 explants were processed;

[0048] 2. Induction of callus: remove the petiole explants after detoxification, cut off 0.5 cm at both ends, inoculate in induction medium MS+6-BA 1 mg / L +NAA 0.4 mg / L for adventitious bud induction, Control the culture temperature at 26°C, culture for 1 day under red light irradiation, the red light has electromagnetic radiation with a wavelength of 720nm, and the light intensity is 4800lx; then continue normal culture for 24 days, the light time is 14h / day, and the light ...

Embodiment 3

[0052] Such as figure 1 As shown, the red light irradiation time has a greater induction rate to the explants. With the increase of the light time, the induction rate also increases. 42% increased by 17 percentage points, and continued to increase the red light exposure time, which had no significant impact on the induction rate. During the induction process of adventitious buds, the present invention pioneered the use of high-intensity red light treatment, which greatly increased the induction rate of adventitious buds. The possible reason is that a certain intensity of infrared light can activate and induce explant callus adventitious buds The enzyme system required for germination, thus promoting the induction rate of callus adventitious shoots, may also promote the induction of 6-BA and NAA on explants. However, red light treatment in the process of differentiation culture and root induction has no positive effect on the differentiation rate and rooting rate. The present...

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Abstract

The invention provides a method for obtaining regenerated plants by inducing calluses by using petioles of Caihong populus as explants. The method comprises the following steps of firstly, selecting explants, disinfecting with 0.1% HgCl2 for 12 minutes, inoculating into an induction culture medium, carrying out callus induction, controlling the culture temperature at 24-26 DEG C, culturing at theillumination time of 10-14h/day and the illumination intensity of 1400-1800lx, transferring obtained Caihong populus calluses into a differential medium to be cultured for 30 days, and finally, inoculating aseptic seedlings of the Caihong populus into a rooting medium to be subjected to rooting culture for 25 days. The method can effectively solve the problem of in-vitro rapid propagation of the Caihong populus.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of poplar poplar, in particular to a method for establishing a regeneration system of poplar poplar with petioles as explants, and belongs to the technical field of seedling propagation and breeding of woody ornamental plants. Background technique [0002] Caihong Yang, belonging to the genus Populus of Salicaceae, is a new gene-induced budding variety of Populus americana 2025. The color of the branches and leaves in the growth period of the whole year is bright red-light yellow-orange-yellow green from top to bottom. At present, this variety mainly adopts two methods of cutting and grafting for plant propagation. The precautions required for cutting seedlings, preparation of cuttings, cutting time, cutting density and seedling management techniques in the process of growing seedlings by cuttings are basically the same as those of other poplar varieties. When grafting seedlings, th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 束晓春庄维兵王忠张凤姣王涛王宁
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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