Rabdosia rubescens stem aqueous extract powder as well as preparation, anti-osteoporosis effect and application thereof

A technology of rubescens and water extract, applied in the field of biomedicine, can solve the problem of unknown biological activity of all components and the like

Inactive Publication Date: 2021-02-12
北京恒润泰生医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological activity of a single component of the aqueous extract powder of the stem of Possinthes hibiscus was reported, the combined biological activity of the whole component was not known.

Method used

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  • Rabdosia rubescens stem aqueous extract powder as well as preparation, anti-osteoporosis effect and application thereof
  • Rabdosia rubescens stem aqueous extract powder as well as preparation, anti-osteoporosis effect and application thereof
  • Rabdosia rubescens stem aqueous extract powder as well as preparation, anti-osteoporosis effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 prepares the water extract powder of the stem of Radix radix L.

[0043] Take 300g of dried Radix japonicus stalks, wash them with tap water, and chop them up. The chopped stems were heated in 700-1200 mL of distilled water at 50 °C-100 °C for 0.5-4 h while stirring (200-400 rpm). Cooled to room temperature, filtered, and the filtrate was concentrated under reduced pressure to obtain 72 g of water extract powder of the stems of Radix sativa.

Embodiment 2

[0044] Embodiment 2 Measures the chromatogram and mass spectrometry ion current spectrum of Bossium radix stem aqueous extract powder

[0045] 2-1. Preparation of sample solution (10mg / mL)

[0046] Weigh 26.7mg of the water extract powder of the stems of Boswellia japonicus, and dissolve the powder in 2.67mL of ultrapure water. The resulting solution was ultrasonically shaken for 1 minute, and then centrifuged at 13000rpm for 10 minutes. Take the supernatant, pass through a 0.22μm filter membrane, and place it in a sample bottle for chromatography and mass spectrometry.

[0047] 2-2 Chromatographic conditions

[0048] Chromatographic column: Waters, Acquity HSS T3 column (2.1×100mm i.d., 1.7μm); injection volume: 2μL; PDA detector: 190-400nm; mobile phase: water (0.1% formic acid), acetonitrile; use this mobile phase and follow the gradient in Table 1 Wash the column.

[0049] Table 1 Mobile phase gradient table

[0050]

[0051] 2-3. Measurement chromatogram

[005...

Embodiment 3

[0057] Embodiment 3 specifies the structure of 28 kinds of components in the water extract powder of Rubescens sinensis stem

[0058] The UPLC chromatography of Example 2 was coupled with mass spectrometry, and the UPLC-mass spectrometry of the water extract powder of the stem of Radix Orbis lentils was determined. The mass spectrometry conditions are two modes of electrospray ionization, positive ion and negative ion. Ion mode parameters: capillary voltage is 1000V, desolvation gas flow rate is 800L / h, temperature is 450°C, source temperature is 120°C, cone gas flow rate is 50L / h, spray pressure is 6bar, fragmentation voltage is 20-45V, The sampling cone voltage is 6V, the acquisition mode is MSE continuum resolution mode, the data acquisition range of the mass number to charge number ratio (m / z) of charged particles is 100-1500, the low energy channel Trap fragmentation voltage is 6V, and the high energy channel The channel Trap fragmentation voltage selection gradient volt...

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PUM

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Abstract

The invention discloses rabdosia rubescens stem aqueous extract powder, a preparation method thereof and an application of the rabdosia rubescens stem aqueous extract powder in treating osteoporosis.The rabdosia rubescens stem aqueous extract powder prepared by the method provided by the invention contains 17 quinic acid compounds and 11 quercetin compounds. Experiments prove that the rabdosia rubescens stem aqueous extract powder disclosed by the invention has an excellent anti-osteoporosis effect. Therefore, the invention provides an effective technical means for osteoporosis treatment.

Description

technical field [0001] The present invention relates to a method for preparing the water extract powder of the stem of Ribesia japonicus, and relates to the application of the water extract powder of the stem of Ribesia japonicus prepared by the method in the preparation of anti-osteoporosis drugs. The invention belongs to the field of biomedicine. Background technique [0002] Whether you are a woman or a man, as long as you live long enough, you will definitely suffer from osteoporosis. No matter who, bone density will reach the highest value around the age of 20. Thereafter, bone density decreases by approximately 1% per year. On average, 80-year-old women have only 40% of their peak bone density. With increasing age and decreasing bone density year by year, bone health declines day by day. When the bone density drops to 25% or less than 25% of the highest bone density, even a very small bone trauma can cause spontaneous fracture. Women and men who like sports natura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P19/10A61K135/00
CPCA61K36/53A61K2236/331A61P19/10
Inventor 彭师奇赵明黄飞卢斌
Owner 北京恒润泰生医药科技有限公司
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