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Composition for preventing, alleviating or treating respiratory diseases, containing siraitia grosvenorii extract as active ingredient

Respiratory diseases, technology of active ingredients, applied in the field of compositions for preventing, improving or treating respiratory diseases containing Luo Han Guo extract as an active ingredient, achieving the effects of reduced tissue damage, excellent inflammation inhibitory activity, useful use

Pending Publication Date: 2021-02-12
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As an example of the known prior art related to Luo Han Guo extract, Korean Laid-Open Patent No. 2015-0051369 discloses a pharmaceutical composition for the prevention and treatment of asthma and atopy containing Luo Han Guo as an active ingredient, but until now So far, there is no known invention related to the composition for preventing, improving or treating respiratory diseases comprising the Luo Han Guo residue extract of the present invention as an active ingredient.

Method used

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  • Composition for preventing, alleviating or treating respiratory diseases, containing siraitia grosvenorii extract as active ingredient
  • Composition for preventing, alleviating or treating respiratory diseases, containing siraitia grosvenorii extract as active ingredient
  • Composition for preventing, alleviating or treating respiratory diseases, containing siraitia grosvenorii extract as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1. prepares Luo Han Guo extract

[0033] After pulverizing 200 g of dried Luo Han Guo, 2 liters of water were added to carry out reflux extraction for 3 hours. After filtering the extract, 1 liter of 70% (v / v) ethanol was added to 50 g of the dried residue obtained by drying the remaining residue, followed by reflux extraction for 3 hours. After filtering the extract, concentrate and dry to prepare the Luo Han Guo residue extract.

Embodiment 2

[0034] Example 2. Analysis of respiratory hyperresponsiveness according to administration of Luo Han Guo residue extract using an animal model of asthma.

[0035] Experiments were performed using an animal model of bronchial asthma induced by ovalbumin. Experimental animals were 7-week-old Balb / c mice provided by Jackson Laboratory (Bar Harbor, ME, USA), which were adapted for 1 week before being used in experiments. 0.26 mg of aluminum hydroxide (A8222, Sigma-Aldrich Company, Missouri (MO), USA) and 12.5 μg of aluminum hydroxide (A5503, Sigma-Aldrich Company) suspended in the abdominal cavity were injected into the abdominal cavity at intervals of 2 weeks to acclimated mice for 1 week. Ovalbumin in 0.25ml of phosphate buffer solution to sensitize it. On the 3rd and 10th days after the first intraperitoneal administration of ovalbumin, after intratracheal injection (intratracheal injection) of 2 mg of ovalbumin to mice, within 4 weeks from the 21st day, use an ultrasonic nebu...

Embodiment 3

[0042] Example 3. Confirmation of the reduction effect of inflammatory cells in bronchoalveolar lavage fluid after administration of Luo Han Guo residue extract to an asthma animal model

[0043] At a time point of 48 hours after the last exposure to ovalbumin in the ovalbumin-exposed animal model, the mice were anesthetized and bronchiectomized. The bronchoalveoli were washed with 1.0 ml of ice-cold (Ice-cold) DMEM medium and the washing solution was collected. Immediately after recovery, the bronchoalveolar washing fluid of each individual was centrifuged to separate blood cells. After staining with 0.04% trypan blue, the total cell number was counted with a hematocytometer, and then coated with a cytocentrifuge. After the Cytospin was used to smear the specimens, Diff-Quick staining (Romanowsky stain (Romanowsky stain)) was carried out, and a 400-fold optical microscope (Lightmicroscope, Nikon (Nikon) Co., Ltd., Japan) ) to identify and count eosinophils and other inflamma...

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Abstract

The present invention relates to a composition for preventing, alleviating or treating respiratory diseases, containing a Siraitia grosvenorii residual extract as an active ingredient. In an ovalbumin(OVA) induced asthma animal model, a Siraitia grosvenorii residual extract of the present invention has effects of reducing airway hypersensitivity, inhibiting inflammatory cells in bronchoalveolar lavage fluid and reducing Th2 cytokines (IL-4, IL-5 and IL-13) in bronchoalveolar lavage fluid, reducing inflammation and tissue damage in lung tissue, inhibiting airway smooth muscle contraction and airway inflammation, inhibiting the expression of IL-13, TARC, TNF-a, IL-17 and mucus gene MUC5AC in lung tissue, and reducing, in alveolar cells, ROS having increased because of fine dust.

Description

technical field [0001] The present invention relates to a composition for preventing, improving or treating respiratory diseases, which contains Luo Han Guo residue extract as an active ingredient. Background technique [0002] Asthma, chronic obstructive pulmonary disease, allergic rhinitis, antitussive and expectorant, acute and chronic bronchitis, bronchiolitis, pharyngitis, tonsillitis, laryngitis, etc. are representative diseases of the respiratory system. Asthma (asthma) refers to chronic inflammation in the airways, especially in the bronchi. Inflammation caused by asthma can be exacerbated by many factors such as soot, allergic antigens, cold wind, exercise, and respiratory tract infection. Continuous inflammation can cause deformation of the airway and hyper-responsiveness of the airway. These causes may cause general symptoms such as wheezing (a symptom of wheezing or grunting breathing sounds due to narrowing of the airway), wheezing, coughing, and excessive phle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A61K36/42A61P11/00
CPCA23L33/105A61K36/42A61P11/00A61P11/06A61K2236/333A23V2002/00A23V2200/314A23L33/40
Inventor 金东善成尹映李允美孙银贞陆兴周李永实
Owner KOREA INST OF ORIENTAL MEDICINE
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