Application of verapamil hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria

A technology of verapamil hydrochloride and plant pathogenic bacteria, applied in the field of agricultural biology, to achieve high quality, broad research and market application prospects, and good inhibitory effect

Active Publication Date: 2021-10-26
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no report on the inhibitory effect of verapamil hydrochloride on agricultural plant pathogens at home and abroad.

Method used

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  • Application of verapamil hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria
  • Application of verapamil hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria
  • Application of verapamil hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1. Experimental materials

[0031] 1. Verapamil hydrochloride was purchased from Sigma Company and stored at 4°C until use.

[0032] 2. In this experiment, the plant pathogenic bacteria were stored in a 4°C refrigerator, and the medium used was potato dextrose medium (referred to as PDA). PDA medium formula: 200 g potato (potato), 20 g glucose, 20 g agar, 1000 ml distilled water, natural pH. Preparation method: Wash and peel the potatoes, weigh 200 g and cut into small pieces, add 1000 ml of distilled water and boil for 20 minutes, then filter it into a large beaker with four layers of gauze, add 20 g of agar and 20 g of glucose according to the needs of the experiment, stir Evenly, add water to 1000 ml, sterilize at 121°C for 20 minutes after aliquoting, cool down and set aside.

[0033] 3. Yeast extract peptone glucose medium (YPD for short) was used to measure the spore germination and germ tube elongation of plant pathogenic bacteria in this experiment. Formula: ...

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Abstract

The invention provides the application of verapamil hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria. The present invention proves that verapamil hydrochloride has the effect on tomato Botrytis cinerea and Fusarium oxysporum through experiments on mycelium growth, spore germination and germ tube elongation of tomato Botrytis cinerea and Fusarium oxysporum. The tomato specialized type has strong inhibitory effect, and it can be prepared as a fungicide for preventing and treating botrytis cinerea and plant diseases caused by Fusarium oxysporum. Traditional pesticides pollute the environment with high residues and threaten food safety. The use of a large number of pesticides also leads to the enhancement of the resistance of pathogenic bacteria. As a fungicide, verapamil hydrochloride has the advantages of high efficiency and low toxicity. It is suitable for the requirements of chemical control of plant diseases and can ensure the high quality of agricultural products and fruits and vegetables. Development requirements, with broad research and market prospects.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology and relates to bactericidal active compounds, in particular to the application of verapamil hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria. Background technique [0002] Botrytis cinerea is a common disease caused by the infection of Botrytis cinerea. It is one of the most harmful diseases to tomato production and occurs very commonly. In addition to harming tomatoes, Botrytis cinerea can also harm many important economic crops such as eggplants, cucumbers, grapes, and strawberries, causing botrytis cinerea in fruits and vegetables. The disease can occur not only during the growing season of the host plant, but also during the storage of agricultural products, so the damage is serious. Especially in recent years, the large-scale promotion of protected cultivation methods has led to a wide range of sources ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N33/10A01P3/00
CPCA01N33/10
Inventor 王光远徐敏梁文星刘炳辰刘美洁
Owner QINGDAO AGRI UNIV
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