Construction method and application of cd155 gene humanized non-human animal

A non-human animal and construction method technology, applied in the field of construction of CD155 genetically modified non-human animal models, can solve the problem that animal models cannot well reflect the real state of the human body.
CN112458117BActive Publication Date: 2021-05-04BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD
Publication Date
2021-05-04

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Abstract

The invention provides a method for constructing a CD155 gene humanized non-human animal, which uses homologous recombination to introduce the nucleotide sequence encoding human CD155 protein into the non-human animal genome, and the animal can normally express human Anti-CD155 protein can be used as an animal model for human CD155 signaling mechanism research, tumor and immune disease drug screening, and has important application value for the development of new drugs for immune targets. The present invention also provides a humanized CD155 protein, a humanized CD155 gene, a CD155 gene targeting vector, and the non-human animal obtained by the above construction method and its application in the field of biomedicine.
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Description

technical field

[0001] The invention belongs to the field of animal genetic engineering and genetic modification, and in particular relates to a method for constructing a CD155 genetically modified non-human animal model and its application in the field of biomedicine. Background technique

[0002] CD155, also known as PVS, HVED, NECL5, TAGE4 or Necl-5, is a Nectin-like type I transmembrane glycoprotein, which belongs to the PVR-related (PRR) family under the immunoglobulin superfamily, and is an adhesion that promotes cell binding Protein, which can bind to poliovirus (PV for short) and allow it to enter susceptible cells, so it is more commonly called PVR (Poliovirus Receptor, poliovirus receptor). CD155 is expressed in monocytes, macrophages, endothelial cells, epithelial cells, CD34+ thymocytes and neurons, but it is highly expressed in most tumor cells, including lung cancer, breast cancer, ovarian cancer and melanoma. Studies have shown that CD155 plays an important r...

Claims

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