Human-derived enterococcus avialis capable of producing gamma-aminobutyric acid and application of human-derived enterococcus avialis

An aminobutyric acid, human-derived technology, applied in the biological field, can solve the problems of few reports on the physiological functions of the body

Active Publication Date: 2021-03-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Enterococcus avium (Enterococcus avium) is a type of Enterococcus, which exists in large quantities in the intestinal tract, but there are few reports on its physiological functions on the body
After searching, there is no report on the isolation and acquisition of human-derived γ-aminobutyric acid (GABA)-producing Enterococcus avium and its application in maintaining the balance of intestinal flora and regulating the health of the body.

Method used

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  • Human-derived enterococcus avialis capable of producing gamma-aminobutyric acid and application of human-derived enterococcus avialis
  • Human-derived enterococcus avialis capable of producing gamma-aminobutyric acid and application of human-derived enterococcus avialis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Screening and identification of physiological and biochemical characteristics of Enterococcus avium strains

[0027] Use a sterile sampler to collect fecal samples from healthy people that have not been exposed to the air, quickly dissolve them in sterile saline, and mix well. Take 100 μL of feces aqueous solution for 10-fold serial dilution, then take 0.5 mL of the diluted solution and spread it on the MRS medium agar plate, incubate at 37±1°C for 24 hours, pick the white circular colony negative for catalase, and repeatedly streak After the line was purified, a strain with an oval shape was obtained, which was named MRS4-2.

[0028] Among them, the formula of the above MRS medium: tryptone 10.0g, beef extract 10.0g, yeast powder 5g, glucose 20.0g, triammonium citrate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, sodium acetate 3.12g, phosphoric acid Sodium hydrogen disodium 1.63g, potassium acetate 2.25g, Tween-80 1.0mL, distilled water 1000mL, a...

Embodiment 2

[0034]Embodiment 2: the application of Enterococcus avium (Enterococcusavium) CGMCC No.20990 in the production of gamma-aminobutyric acid (GABA) by using glutamic acid-added medium

[0035] 2% overnight cultured Enterococcus avium (Enterococcusavium) CGMCC No. 20990 was transferred to MRS medium (ie GMRS medium) containing 1% glutamic acid, and cultured at 37° C. for 120 hours.

[0036] The formula of the above GMRS medium: tryptone 10.0g, beef extract 10.0g, yeast powder 5g, glucose 20.0g, triammonium citrate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, sodium acetate 3.12g, dihydrogen phosphate Sodium 1.63g, potassium acetate 2.25g, Tween-80 1.0mL, sodium glutamate 10.0g, distilled water 1000mL, sterilized at 115°C for 30 minutes.

[0037] The fermentation broth was centrifuged at 6000 g for 5 minutes, and the supernatant was collected. After the supernatant was sterilized by a 0.22 μm sterile filter, its GABA content was determined to be 1.851±0.205 g / L.

[003...

Embodiment 3

[0040] Example 3: Application of Enterococcus avium (Enterococcusavium) CGMCC No.20990 in the production of gamma-aminobutyric acid (GABA) by adding soybean powder enzymatic hydrolyzate

[0041] Transfer 2% of overnight cultured Enterococcus avium (Enterococcusavium) CGMCC No. 20990 to MRS medium containing 5% soybean powder enzymatic hydrolyzate by volume, and culture it statically at 37°C for 72 hours.

[0042] The formula of the above MRS medium: tryptone 10.0g, beef extract 10.0g, yeast powder 5g, glucose 20.0g, triammonium citrate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, sodium acetate 3.12g, dihydrogen phosphate Sodium 1.63g, potassium acetate 2.25g, Tween-80 1.0mL, distilled water 1000mL, agar 20.0g should be added to the solid, and sterilized at 115°C for 30 minutes.

[0043] The preparation method of the above soybean powder enzymatic hydrolysis solution: dissolve 5 grams of soybean powder in 10 ml of 0.05% NaCl solution, add pepsin to a final concentra...

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Abstract

The invention discloses human-derived enterococcus avialis capable of generating gamma-aminobutyric acid (GABA). The human-derived enterococcus avialis strain is named as Enterococcusavium MRS4-2, theenterococcus avialis strain is preserved in China General Microbiological Culture Collection Center on November 30, 2020, and the preservation number is CGMCC No.20990. The invention also discloses an application of the enterococcus avialis to production of the gamma-aminobutyric acid by using a culture medium added with glutamic acid or glutamate or an MRS culture medium added with soybean mealenzymatic hydrolysate. Experiments show that the GABA generated by the enterococcus avium has an adjusting effect on intestinal flora, which indicates that the GABA has a probiotic effect in the aspects of maintaining intestinal flora balance, adjusting body health and the like, and besides, the fact that the enterococcus avium has application potential in development of intestinal health micro-ecological products and probiotic fermentation products is also confirmed.

Description

technical field [0001] The present invention relates to a kind of Enterococcus avium and its application, in particular to a strain of Enterococcus avium isolated from healthy human feces and producing γ-aminobutyric acid (gamma-aminobutyric acid, GABA) and its application, which belongs to biotechnology field. Background technique [0002] γ-Aminobutyric acid (GABA) is a naturally occurring amino acid that is not composed of proteins. It is widely found in animals and plants. It acts as an inhibitory neurotransmitter and participates in various metabolic activities. , anti-inflammatory, anti-anxiety, diuretic and improve sleep and other physiological functions. With the deepening of research, GABA has been used as a new bioactive factor in food, medicine and feed industries, and has been approved as a new resource food (Ministry of Health Announcement No. 12 in 2009). Many microorganisms including mold, yeast, bacillus, Escherichia coli and lactic acid bacteria can conver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/00C12R1/01
CPCC12N1/20C12P13/005
Inventor 郭婷婷孔健顾心怡
Owner SHANDONG UNIV
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