Amino acid-loaded modified amnion mask and preparation method thereof

A technology of amino acid and amniotic membrane, applied in skin care preparations, pharmaceutical formulas, cosmetic preparations, etc., can solve the problems of safety and effectiveness beyond doubt, and achieve the effect of improving skin immunity, wide sources, and diluting scars

Pending Publication Date: 2021-03-19
郑楠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, animal (sheep, goat, deer) amnion has a wide range of sources and low cost; secondly, there is no rejection reaction caused by inter-species differences, and the greater the inter-species differences, the better the biological effect; third, a large number of domestic and foreign Sheep placenta products (such as various placenta extracts, freeze-dried powder, capsules, oral liquids and external products, etc.) are used for human beings through injection, oral administration, and external application. Their safety and effectiveness are unquestionable. There is technology to use amniotic membrane as the raw material for the preparation of the mask

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Take goat placenta as an example below.

[0047] (1) Separation of amnion and chorion:

[0048] The healthy goat placenta was washed 3 times with water first, and then washed 2 times with phosphate buffered saline pre-cooled at 4°C; the amnion and chorion were bluntly separated, and the separated amnion and chorion were washed with distilled water 3 times again.

[0049] (2) Amniotic membrane pretreatment:

[0050] ①Preparation of 1% TritonX-100: Mix TritonX-100 stock solution and PBS at a volume ratio of 3:7, place in a 37°C water bath for 3 hours to fully dissolve and mix, and prepare a 30% TritonX-100 stock solution; This stock solution was diluted 30 times to make 1% TritonX-100 solution;

[0051] ② Amniotic membrane pretreatment: soak the isolated amniotic membrane in 1% TritonX-100PBS solution, shake at 4°C for 24 hours, take it out and wash it.

[0052] Treating the amniotic membrane with TritonX-100 has the effect of dissolving lipids and increasing the perme...

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Abstract

The invention discloses an amino acid-loaded modified amnion mask and a preparation method thereof. The method comprises the steps of separating the amnion and chorion of the placenta of a healthy animal (sheep, goat or deer); performing decellularization treatment on the amnion to form an amnion acellular matrix; performing freeze-thaw crushing, centrifugal separation and other processes on the chorion to prepare a chorion complete amino acid nutrition solution; and adding amino acid to the amnion acellular matrix in an adsorption form to form modified amnion, and bonding the modified amnionwith a mask carrier to form the amino acid-loaded modified amnion mask. The mask is natural and free of additives, has remarkable beautifying and skin-care functions of nourishing the skin, replenishing water, removing wrinkles, fading scars and acne marks, regulating skin metabolism, improving skin immunity and the like, and has a promoting effect on skin recovery after burns and scalds.

Description

technical field [0001] The invention relates to the technical field of beauty and skin care products and preparation methods of the beauty and skin care products, in particular to a modified amniotic membrane mask loaded with amino acids and a preparation method thereof. Background technique [0002] The mask raises the temperature of the skin by blocking the contact between the skin and the air, inhibiting sebum secretion and sweat evaporation. After the skin temperature rises, it will promote blood circulation, so that the active ingredients in the mask can penetrate into the epidermis or deeper and wider places. Accelerated blood circulation will make the skin rosy and shiny, and after the skin absorbs more water, nutrient essence and various active ingredients, it can effectively improve skin dehydration and dullness, reduce the formation of fine lines, delay skin aging, and to a certain extent It has the effect of removing freckles and acne; using the mask can also soft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/44A61K8/02A61Q19/00A61Q19/08A61P17/02
CPCA61K8/0212A61K8/44A61K8/982A61Q19/00A61Q19/08A61P17/02
Inventor 郑楠
Owner 郑楠
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