miRNA related to chicken slaughter traits and its application
A character and specific technology, applied in the field of miRNA and its application, to achieve the effect of simple and fast detection method
Active Publication Date: 2022-04-15
YANGZHOU UNIV
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Problems solved by technology
[0005] At present, there are few reports on miRNAs related to chicken slaughter traits, so it is particularly important to identify new miRNAs to study chicken development, and to develop auxiliary selection markers for chicken slaughter traits
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Embodiment 1
[0025] Example 1 Based on the joint analysis of RNA-seq and mRNA, the miRNA affecting chicken slaughter traits---Novel-gga-miR-158 was obtained
[0026] 1. Collection of sequencing samples
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The present invention discloses a miRNA related to chicken slaughtering traits. The miRNA is Novel-gga-miR-158, and its nucleotide sequence is shown in SEQ ID NO.1. The invention also discloses specific primers for detecting the expression level of the miRNA related to chicken slaughter traits. The invention also discloses a detection kit and a detection method thereof. The invention also discloses the application of the miRNA, specific primers, and the detection kit in developing auxiliary selection markers for slaughtering traits of chickens. Finally, the invention discloses a method for screening or cultivating chickens with excellent slaughter performance. The detection kit provided by the invention can be used to detect the expression level of Novel-gga-miR-158 in chicken muscle tissue, and the detection method is simple and quick. The present invention can also utilize the expression level of the Novel-gga-miR-158 to screen or breed chickens with excellent slaughter performance.
Description
technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to miRNAs related to slaughtering traits of chickens and applications thereof. Background technique [0002] The poultry industry is an important part of the livestock industry. Yellow-feather broiler is derived from our country's native poultry species, and it has a unique flavor and better meat quality than white-feather broiler. In my country, the output of yellow-feathered broilers has increased year by year, and has been basically equal to that of white-feathered broilers in the past two years. However, yellow-feathered broilers have deficiencies in muscle yield, growth rate and feed utilization, and there is still great potential for improvement. [0003] miRNA is a non-coding RNA with a length of about 20nt, which performs post-transcriptional regulation by binding to the 3′UTR region of the target gene. A large number of studies have shown that miRNA plays an imp...
Claims
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IPC IPC(8): C12Q1/6888C12N15/113
CPCC12Q1/6888C12Q2600/158C12Q2600/178
Inventor 张跟喜张欣超李培峰吴鹏飞何明亮张丽魏清宇周楷智张涛谢恺舟戴国俊王金玉
Owner YANGZHOU UNIV
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