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MiRNA related to chicken slaughter traits and application thereof

A technology for slaughter performance and traits, applied in the field of miRNA and its application, to achieve the effect of simple and fast detection method

Active Publication Date: 2021-03-30
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on miRNAs related to chicken slaughter traits, so it is particularly important to identify new miRNAs to study chicken development, and to develop auxiliary selection markers for chicken slaughter traits

Method used

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  • MiRNA related to chicken slaughter traits and application thereof
  • MiRNA related to chicken slaughter traits and application thereof
  • MiRNA related to chicken slaughter traits and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Based on the joint analysis of RNA-seq and mRNA, the miRNA affecting chicken slaughter traits---Novel-gga-miR-158 was obtained

[0026] 1. Collection of sequencing samples

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PUM

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Abstract

The invention discloses miRNA related to chicken slaughter traits, the miRNA is Novel-gga-miR-158, and the nucleotide sequence of the miRNA is shown as SEQ ID NO. 1. The invention also discloses a specific primer for detecting the expression quantity of the miRNA related to the chicken slaughter traits. The invention also discloses a detection kit and a detection method thereof. The invention alsodiscloses the miRNA, a specific primer and an application of the detection kit in developing an auxiliary selection marker of chicken slaughter traits. The invention finally discloses a method for screening or breeding chickens with excellent slaughter performance. The detection kit can be used for detecting the expression quantity of Novel-gga-miR-158 in chicken muscle tissues, and the detectionmethod is simple and rapid. According to the invention, the expression quantity of the Novel-gga-miR-158 can also be utilized to screen or breed chickens with excellent slaughter performance.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to miRNAs related to slaughtering traits of chickens and applications thereof. Background technique [0002] The poultry industry is an important part of the livestock industry. Yellow-feather broiler is derived from our country's native poultry species, and it has a unique flavor and better meat quality than white-feather broiler. In my country, the output of yellow-feathered broilers has increased year by year, and has been basically equal to that of white-feathered broilers in the past two years. However, yellow-feathered broilers have deficiencies in muscle yield, growth rate and feed utilization, and there is still great potential for improvement. [0003] miRNA is a non-coding RNA with a length of about 20nt, which performs post-transcriptional regulation by binding to the 3′UTR region of the target gene. A large number of studies have shown that miRNA plays an imp...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/113
CPCC12Q1/6888C12Q2600/158C12Q2600/178
Inventor 张跟喜张欣超李培峰吴鹏飞何明亮张丽魏清宇周楷智张涛谢恺舟戴国俊王金玉
Owner YANGZHOU UNIV