System and method for non-invasive embryo transplantation priority rating

A priority, embryo technology, applied in the field of assisted reproduction, can solve problems such as lack of embryo verification research, achieve the effect of improving the success rate and reducing the cancellation rate of FET cycles

Active Publication Date: 2021-03-30
XUKANG MEDICAL SCI & TECH (SUZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, large-scale embryo validation studies are still lacking
There is an unmet need in the field for an effective embryo implantation prioritization system validated in large-scale experiments and blinded clinical trials

Method used

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  • System and method for non-invasive embryo transplantation priority rating
  • System and method for non-invasive embryo transplantation priority rating
  • System and method for non-invasive embryo transplantation priority rating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0266] Training data set construction and random forest model establishment

[0267] Embryo culture fluid collection

[0268] In 2017, a total of 345 embryo culture fluids were collected from Nanjing Jinling Hospital Reproductive Medicine Center, Changzheng Hospital and Nanjing Drum Tower Hospital. The approval of the Institutional Ethics Review Board (IRB) [Reference number: 2016NJKY-028, CZEC (2017-06), 2017-09-02] and informed consent were obtained before the study was carried out. The age of female patients is 20-45 years old. All embryos were ICSI (cytoplasmic sperm injection) embryos cultured in vitro to the cleavage stage (D3); after washing, they were transferred to a drop of fresh blastocyst medium (SAGE, Cooper Surgical Fertility Co., Denmark). Morphological parameters of early cleavage by Gardner et al. -stage embryos that correlate with fetal development and delivery: prospective and applied data for increased pregnancy rates. Hum. Reprod. 22, 230-240 (2007)),...

Embodiment 2

[0304] Embryo rating system based on model-predicted euploid probability

[0305] Model-Based Prediction of Euploid Probability

[0306] With the advent of the comprehensive chromosome screening (CCS) method, this method has been used to select euploid embryos for clinical embryo transfer. The ploidy of an embryo can be indicated by the pattern of copy number abnormalities (CNV) in the embryo. Traditionally, the cutoff value of chromosome copy number gain / loss is set to report euploid, aneuploid or mosaic embryos (A.R.Victor et al., One hundred mosaic embryos transferred prospectively in asingle clinic: exploring when and why they result in healthy pregnancies. Fertil. Steril. 111, 280-293 (2019)). However, DNA molecules in embryo culture fluid are highly fragmented and degradable in nature, and this relatively arbitrary way of sorting embryos, when applied to chromosome screening of embryo culture fluid samples, may lead to high aneuploidy / mosaicism ratio (H. Bolton et a...

Embodiment 3

[0324] A prospective observational study validates a rating system-based embryo screening strategy

[0325] patient selection

[0326] All oocyte recovery cycles and single blastocyst resuscitation and transplantation cycles were carried out in the Reproductive Medicine Center of Nanjing Jinling Hospital from July 2017 to December 2018, and NICS screening was performed. This study was approved by the Ethics Committee of Nanjing Jinling Hospital (volume number 2016NJKY-028), and written informed consent was obtained before the study.

[0327] Inclusion criteria were as follows: maternal age 20 years; embryos from ICSI (intracytoplasmic sperm injection) and cultured to the blastocyst stage (D5 / 6), blastocysts were collected in all cycles for culture liquid; between July 2017 and December 2018, at least one cycle of blastocyst resuscitation and transfer was performed.

[0328] Exclusion criteria include: patients with abnormal uterine cavity shape, endometrial injury, intraut...

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Abstract

The invention discloses a system and method for non-invasive embryo transplantation priority rating, provides a combination of biometric and biometric features, and can be used for embryo euploid probability prediction and embryo implantation priority rating based on embryo culture fluid and / or blastocyst fluid. The invention further relates to a method of inputting the magnitudes of the biometricfeatures into a classification system, such as a random forest, to predict the embryo euploid probability, and to a method of rating the implantation priority of embryos by using the embryo euploid probability. The invention also provides application of an agent for determining the biological characteristics and combinations of biological characteristics in the prediction and rating methods of the invention, as well as a system for the prediction method and the rating method and a computer program product.

Description

[0001] The present invention relates to the field of assisted reproductive technology, and more particularly to non-invasive embryo screening (NICS) methods for prioritizing embryo transfer in in vitro fertilization-embryo transfer (IVF-ET), and associated biometric combinations, and classification , forecasting and rating systems and computer program products. Background technique [0002] In vitro fertilization-embryo transfer (IVF-ET) is increasingly being adopted for the treatment of infertility. Every year, millions of IVF-ET are performed around the world. With traditional IVF-ET procedures, more than one embryo is usually transferred per treatment cycle, which results in a multiple pregnancy rate of approximately 20%. Multiple gestation increases the incidence of adverse events that may compromise maternal and fetal health. Elective single embryo transfer (eSET) is the most effective method to reduce multiple pregnancy and has been increasingly accepted internationall...

Claims

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Application Information

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IPC IPC(8): G16B20/20G16B30/00G16B40/00
CPCG16B20/20G16B30/00G16B40/00
Inventor 邹央云姚雅馨陆思嘉薄世平夏滢颖
Owner XUKANG MEDICAL SCI & TECH (SUZHOU) CO LTD
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