Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-efficiency method for inducing and differentiating human mesenchymal stem cells into lipid

A technique for inducing differentiation of mesenchymal stem cells, which is applied in the field of high-efficiency human mesenchymal stem cells to induce differentiation into lipids to reduce damage and improve efficiency.

Active Publication Date: 2021-04-13
华夏源(上海)生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the advancement of science and technology, researchers are committed to the development of mesenchymal stem cells, mainly because of their more clinical applications, and they need to play a greater role in overcoming some intractable diseases that humans are currently facing.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat, comprising the following steps:

[0050] S01: cell culture: human mesenchymal stem cells were added into MM3 medium and cultured for 1 day.

[0051] S02: Inducing cell differentiation: adding a lipid-inducing reagent to the medium to induce differentiation of mesenchymal stem cells, and changing the lipid-inducing reagent every 48 hours; inducing differentiation for 7 days;

[0052] S03: Judging the degree of cell differentiation: After the lipid droplets appeared in the differentiated cells, Sudan Red 5B was added to identify the degree of cell differentiation.

[0053] MM3 medium is a medium composed of serum replacement and mesenchymal stem cell basal medium; the volume ratio of serum replacement and mesenchymal stem cell basal medium is 1:19.

[0054]Sudan Red 5B, purchased from Israel Biological Industries (BioInd) company; mesenchymal stem cell basal medium, model 05-2...

Embodiment 2

[0056] A high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat, the specific implementation of which is the same as in Example 1, except that the volume ratio of serum replacement and mesenchymal stem cell basal medium is 1:15 .

Embodiment 3

[0058] A high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat, its specific implementation is the same as in Example 1, the difference from Example 1 is that the time for inducing differentiation in step S02 is 10 days.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology, and particularly relates to a high-efficiency method for inducing and differentiating human mesenchymal stem cells into lipid. The high-efficiency method for inducing and differentiating the human mesenchymal stem cells into the lipid comprises the following steps of: S01, cell culture; S02, cell induced differentiation; and S03, cell differentiation degree judgment. By the method for inducing and differentiating the human mesenchymal stem cells into the lipid, differentiation can be carried out efficiently and quickly, and the phenomenon of reduction in a cell differentiation process is avoided; by the method, large-area lipid droplets can be guaranteed to appear after differentiation for 7 days, and cell differentiation can be carried out smoothly; and by the method, the cell culture and differentiation efficiency is improved, and influence of substances such as carbon dioxide in air on a culture medium and damage to a cell culture process are reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat. Background technique [0002] Mesenchymal stem cells are a kind of pluripotent stem cells, which have all the common characteristics of stem cells, that is, self-renewal and multidirectional differentiation capabilities. With the advancement of science and technology, researchers are committed to the development of mesenchymal stem cells, mainly because they are widely used in clinical practice, and they need to play a greater role in overcoming some intractable diseases that humans are currently facing. [0003] The research on the differentiation of mesenchymal stem cells into lipid is also an important research direction. Especially at the current stage with the development of the beauty repair industry, adipose tissue is the source of seed cells for rebuilding and repa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0653C12N2506/1369C12N2506/1384C12N2506/1392C12N2506/025
Inventor 朱灏
Owner 华夏源(上海)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com