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Active ingredient of traditional Chinese medicine for treating eye diseases and its composition

A technology for eye diseases and compositions, applied in the field of medicine

Active Publication Date: 2022-07-22
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the treatment of ocular diseases related to lymphangiogenesis, inflammation or bacterial infection with the composition of fencetin and fencetin in the present application.

Method used

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  • Active ingredient of traditional Chinese medicine for treating eye diseases and its composition
  • Active ingredient of traditional Chinese medicine for treating eye diseases and its composition
  • Active ingredient of traditional Chinese medicine for treating eye diseases and its composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Qinpi extract and Qinbing eye drops used in the following examples were from Longhua Hospital, Qinpisu was purchased from Chengdu Desit Biotechnology Co., Ltd., Qinpi A and Qinpi B were purchased from Shanghai McLean Biochemical Technology Co., Ltd. Borneol was purchased from Aladdin Reagent (Shanghai) Co., Ltd., D-borneol was purchased from Shanghai Merrill Chemical Technology Co., Ltd., Macrophage Raw264.7 (ATCC TIB-71) cells were purchased from Cell Bank of Chinese Academy of Sciences, mice It was purchased from the Laboratory Animal Center of Shanghai University of Traditional Chinese Medicine (Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd.). RAW264.7 cell culture medium was DMEM medium (Thermo Fisher Biochemical (Beijing) Co., Ltd.) supplemented with 10% fetal bovine serum (FBS) (Serana Europe) and 1% penicillin-streptomycin double antibody (Life Technologies).

[0037] Preparation of qinpisin: 5 mg of qinpisin was dissolved in 60 μl DMSO to make a 4...

Embodiment 2

[0054] The expression of inflammation-related factors in macrophage RAW264.7 was induced by LPS (4μg / mL). After the drug was applied to RAW264.7 cells for 3 hours, the cells were collected, total cell RNA was extracted, reverse transcribed into cDNA, and the effect of the drug on the relative expression of inflammatory factors IL-1β and iNOS mRNA was determined by qRT-PCR technology. The β-actin gene was used as an internal control. The experimental groupings are shown in Table 1. The primer sequences are shown in Table 2.

[0055] RESULTS: The screening results of the activity of inhibiting the expression of IL-1β mRNA in macrophages are shown in the appendix. figure 2 . Compared with the model group (group B), Qinpi extract (N, O, P groups), Qinbing eye drops (Q group), Qinpisu (C group) and the composition of Qinpisu and Qinpi B in different proportions ( H, J, K, L, M groups) can significantly inhibit the expression of IL-1β mRNA (P image 3 . Compared with the negat...

Embodiment 3

[0057] The inhibition effects of each group in Table 3 on the growth of methicillin-resistant Staphylococcus aureus (MRSA) and sensitive Pseudomonas aeruginosa (PA14) were tested. After the MRSA and PA14 strains were activated, they were streaked on LB solid plates and cultured at 37°C for 16-18 hours. The colony size was about 1 mm. Pick 5 colonies and dissolve them in 5ml of MHB liquid medium, vortex to mix well, dilute the bacterial solution 1000 times and add it to a 96-well plate, 80 μl per well, and add the drug to be tested according to Table 3. A positive control and a negative control were set at the same time. The positive control of the PA14 strain was 0.0625 μg / ml ciprofloxacin, the positive control of the MRSA strain was 1.25 μg / ml vancomycin, and the negative control group did not contain the test drug / antibiotic. After 16-18 hours of incubation at 37°C, the absorbance at OD600 was measured. The calculation method of relative bacterial number is OD600 of assay w...

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Abstract

The present invention relates to an active ingredient of a traditional Chinese medicine for treating eye diseases and a composition thereof. The invention breaks the conventional cognition in the field, clarifies the actual active ingredients and pharmacological effects of Qinbing eye drops, and confirms that the composition has stable effect and clear mechanism of action, and can inhibit lymphangiogenesis-related factors in macrophages and Expression of inflammatory factors, inhibits the growth of methicillin-resistant Staphylococcus aureus MRSA and Pseudomonas aeruginosa PA14, and inhibits lymphangiogenesis induced by alkali burns in mouse cornea, so it can be used for lymphangiogenesis, inflammation and immune-related eyes disease treatment.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of active ingredients derived from Qinbing eye drops and an active ingredient composition in the treatment of eye-related diseases. Background technique [0002] Qin Bing eye drops formerly known as Qin Pi eye drops, the prescription comes from the late famous Chinese medicine professor Fan Xinfu. It is used as an in-hospital preparation in Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine for conjunctivitis, keratitis and electro-optic ophthalmia, and for chronic conjunctiva. Symptoms such as eye redness, dry eyes, itching, burning and visual fatigue caused by inflammation have a rapid onset, no irritation to the cornea and conjunctiva, reduce drug resistance, and reduce the recurrence rate. Qin Bing eye drops are pure traditional Chinese medicine eye drops made of Qin skin, supplemented with borneol. Modern pharmacological studies have s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/37A61K9/08A61P27/02A61P37/06A61K31/366
Inventor 张建革周昕林国强刘新泉朱玉莹
Owner SHANGHAI UNIV OF T C M
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