APPLICATIONS OF CRISPRi IN HIGH THROUGHPUT METABOLIC ENGINEERING

A modular and constructive technology, applied in the high-throughput field, can solve problems such as expensive, low-efficiency effects, and off-target mutations
CN112703250APending Publication Date: 2021-04-23ZYMERGEN INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZYMERGEN INC
Publication Date
2021-04-23

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Abstract

The disclosure describes methods, compositions, and kits for high throughput DNA assembly reactions in vitro. The disclosure further describes modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer / protospacer adjacent motif sequence combinations. High throughput methods of CRISPRi and CRISPRa are also disclosed.
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Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims the benefit of priority from US Provisional Application No. 62 / 764,672, filed August 15, 2018, which is hereby incorporated by reference in its entirety for all purposes, including all descriptions, references Literature, drawings and claims.

[0003] Government funding

[0004] This invention was made with government support under Agreement No. HR0011-15-9-0014 awarded by DARPA. The government has certain rights in this invention.

[0005] Statement Regarding Sequence Listing

[0006] The Sequence Listing related to this application is provided in text format in lieu of a paper copy and is hereby incorporated by reference into this specification. The name of the text file containing the sequence listing is ZYMR_030_01WO_SeqList_ST25.txt. The text file is 799kb, created on August 14, 2019 and submitted electronically via EFS-Web. technical field

[0007] The present disclosure relates to...

Claims

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