Method for extracting L-type and E-type phytohemagglutinins (PHAs)

A plant lectin and L-type technology, which is applied in the field of extracting L-type and E-type plant lectins, can solve the problem of no great reference value for the extraction and separation of plant lectins, and achieve high purity and simple operation
CN112707958APending Publication Date: 2021-04-27ZHENGZHOU IMMUNO BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHENGZHOU IMMUNO BIOTECH
Publication Date
2021-04-27

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Abstract

The invention relates to the technical field of plant extraction, and discloses a method for extracting L-type and E-type phytohemagglutinins (PHAs). According to the method, crude protein extraction is carried out through the steps of soaking, crushing, centrifuging, ammonium sulfate fractional precipitation and the like on beans, and then L-type and E-type PHAs are separated and purified through hydrophobic chromatography and ion exchange chromatography. The method is simple to operate, and the extracted L-type and E-type PHAs are high in purity and suitable for large-scale production of the L-type and E-type PHAs.
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Description

technical field

[0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting L-type and E-type lectins. Background technique

[0002] Phytohemagglutinin (Phytohemagglutinin PHA) is a sugar-containing protein extracted from the seeds of legumes. PHA is further divided into E-type and L-type, among which PHA-E type has erythrocyte agglutination; PHA-L type can stimulate lymphocyte transformation and is a stimulator of human lymphocyte mitosis, that is, under the action of PHA-L, it was originally in G0 Lymphocytes transform into lymphoblasts and undergo mitosis.

[0003] There are also methods for extracting phytohemagglutinin PHA in the existing patented technology, and a variety of methods for extracting phytohemagglutinin from leguminous plants have been disclosed so far. For example, the Chinese invention patent application with the publication number CN101508730A discloses a method for extracting bean lectins, which u...

Claims

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