Method for on-line determination of phthalate metabolite content in urine and application

A technology of phthalates and phthalates, which is applied in the field of online determination of phthalates metabolites in urine, can solve unfavorable human sample screening, cumbersome method procedures, and large amount of solvents, etc. problems, to achieve the effect of omitting pretreatment steps, reducing exposure risk, and reducing solvent consumption

Pending Publication Date: 2021-05-11
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many literatures have reported that different methods are applied to the analysis of mPAEs in urine. Most of these methods require some pretreatment of urine. Collection and purification, the method process is cumbersome, the amount of solvent is large, time-consuming and inefficient, and it is not conducive to large-scale human sample screening

Method used

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  • Method for on-line determination of phthalate metabolite content in urine and application
  • Method for on-line determination of phthalate metabolite content in urine and application
  • Method for on-line determination of phthalate metabolite content in urine and application

Examples

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Embodiment 1

[0034] 1. Take 300-500μL urine sample and put it into a 1.5-5mL microcentrifuge tube, add mPAEs isotope internal standard (20ng 13 C 4 -mMP, 20ng 13 C 4 -mEP, 20ng 13 C 4 - mBzP, 10ng 13 C 4 -mOP, 10ng 13 C 4 -mEHP, 10ng 13 C 4 -mEOHP, 10ng 13 C 4 -mEHHP, 10ng 13 C 4 -mnBP, 10ng miBP-d 4 ) Add 5 μL of β-glucuronidase-arylsulfatase and mix well, enzymolyze on a constant temperature shaker at 37°C for 11.5-13.5 hours, add 300-600 μL of acetonitrile to precipitate protein after enzymolysis, vortex mix for 1-6min, Then carry out high-speed refrigerated centrifugation (12000rpm, 5-30min, 4-25°C), and take the supernatant for sampling after centrifugation.

[0035] 2. The model of the online solid phase extraction-liquid chromatography-triple quadrupole mass spectrometry (LC-MS / MS) is Agilent 1260-6470 series. When using automatic valve switching for online solid phase extraction, the six-way valve connects 1 and 6 ( figure 1 Position 1), the injection volume is 300...

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Abstract

The invention belongs to the technical field of organic pollutant analysis, and discloses a method for on-line determination of phthalate metabolite content in urine and application, the method comprises the following steps: adding an isotope internal standard into the urine, then adding beta-glucuronidase-aryl sulfatase, carrying out enzymolysis on a constant-temperature shaking table, adding an organic solvent to precipitate protein after enzymolysis, freezing and centrifuging, transferring the centrifuged supernate, switching through an automatic valve to carry out online solid-phase extraction, and then carrying out quantitative determination through liquid chromatography and triple quadrupole mass spectrometry; and carrying out data processing and quantitative calculation to obtain the content of the phthalate metabolite in the urine. According to the invention, the rapid (20-28min) accurate detection of the phthalate metabolite in the urine of the human body is realized, and an effective method means is provided for human body exposure health risk assessment of phthalate.

Description

technical field [0001] The invention belongs to the technical field of organic pollutant analysis, and more specifically relates to a method and application for online determination of the content of phthalate metabolites in urine. Background technique [0002] Phthalates (PAEs) are ubiquitous pollutants in the environment and are often used in the production of everyday products. According to their relative molecular weight, PAEs can be divided into high molecular weight PAEs and low molecular weight PAEs. High molecular weight PAEs are mainly used as plasticizers to improve the ductility and durability of products; while low molecular weight PAEs mainly make products have fragrance, color and gloss. Toys, food packaging, vinyl flooring, lubricants, adhesives, detergents, personal care products, cosmetics, drug coatings and medical devices in our daily life all contain one or more PAEs. PAEs can enter the human body through atmospheric breathing, dietary intake and skin a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor岳聪聪胡欣黄森源马盛韬杨彦余应新安太成
OwnerGUANGDONG UNIV OF TECH