Method for in-situ determination of activity of drinking water biological activated carbon TTC-dehydrogenase

A technology of biological activated carbon and dehydrogenase, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of destruction, microbial poisoning, damage, etc., and achieve the effect of simple operation method, low operation cost and wide application prospect

Inactive Publication Date: 2017-08-22
HARBIN INST OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

In addition, too strong ultrasonic waves have a certain destructive effect on microbial cell tissue, and the organic solvent in the extraction mixture has a certain toxic effect on microorganisms, which will damage the microbial activity during the transfer from the activated carbon filter material to the solution. , making it difficult to detect its biological activity in subsequent assays, the detection line is high, and the detection biomass is 10 5 CFU / g activated carbon

Method used

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  • Method for in-situ determination of activity of drinking water biological activated carbon TTC-dehydrogenase
  • Method for in-situ determination of activity of drinking water biological activated carbon TTC-dehydrogenase

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specific Embodiment approach 1

[0032] Specific embodiment one: the present embodiment is a kind of method for in situ determination of drinking water biological activated carbon TTC-dehydrogenase activity is carried out according to the following steps:

[0033] 1. Use the absorbance value as the abscissa and the TF concentration as the ordinate to draw the TF standard curve;

[0034] 2. Sample treatment: Take out the activated carbon filter material to be tested from the water, wash it repeatedly with distilled water for 3 to 4 times, then divide the washed activated carbon filter material into 2 parts according to the mass, and put them into two plugged colorimetric tubes respectively. middle;

[0035] 3. Add Tris-HCL buffer solution and 0.36% Na in the two stoppered colorimetric tubes in step 2. 2 SO 3 Solution, glucose solution with a concentration of 0.1mol / L and TTC solution with a mass concentration of 0.4% until the activated carbon filter material is completely submerged, then add formaldehyde so...

specific Embodiment approach 2

[0046] Specific embodiment two: the difference between this embodiment and specific embodiment one is: draw TF standard curve in step one and carry out according to the following steps:

[0047] 1) Pipette 1mL, 2mL, 3mL, 4mL, 5mL and 6mL of TTC standard use solution into six 50mL volumetric flasks, dilute to volume with water, and prepare 20μg / mL, 40μg / mL, 60μg / mL, 80μg / mL respectively mL, 100 μg / mL and 120 μg / mL TTC solutions; the preparation method of the TTC standard use solution is as follows: Weigh 100 mg of TTC solid, dissolve and set the volume to a 100 m volumetric flask to obtain the TTC standard use solution;

[0048] 2) Take 7 test tubes, add 2mL of Tris-HCL buffer respectively, then add 1mL of TTC solution of different concentrations in step (1) to 6 of them, and add 1mL of anaerobic water to the 7th test tube as a control , and then add 1mL of 10% sodium sulfide solution to the 7 test tubes, put the 7 test tubes into a 37°C constant temperature shaker and shake fo...

specific Embodiment approach 3

[0050] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 4, the stoppered colorimetric tubes of the test group and the blank group were placed in a constant temperature water bath shaker with a temperature of 37° C. for 20 hours. Others are the same as the first embodiment.

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Abstract

The invention relates to a method for in-situ determination of the activity of drinking water biological activated carbon TTC-dehydrogenase, and relates to a method for determining the activity of the drinking water biological activated carbon TTC-dehydrogenase. The method aims to solve the technical problem that in an existing method for indirect determination of the activity of the drinking water biological activated carbon TTC-dehydrogenase, the microbial activity is damaged in the process of transferring the drinking water biological activated carbon TTC-dehydrogenase from an activated carbon filter material to a solution, and accordingly the biological activity of the drinking water biological activated carbon TTC-dehydrogenase is difficult to detect in the subsequent determination. The method comprises the first step of drawing a TF standard curve, the second step of processing a sample, the third step of carrying out sample culture, the fourth step of conducting sample culture, the fifth step of stopping an enzyme reaction, the six step of carrying out colorimetric analysis, and the seventh step of calculating the activity of TTC-dehydrogenase. The method has the advantage that the activity of the activated carbon TTC-dehydrogenase can be detected more easily, activated carbon with the biomass being 4.5*10<4>CFU / g can be detected, the step of pretreating an activated carbon sample is omitted, and the operation procedure is simpler and more convenient.

Description

[0001] Technical field: [0002] The invention relates to a method for measuring TTC-dehydrogenase activity of drinking water biological activated carbon. [0003] Background technique: [0004] With the development of drinking water treatment technology, conventional treatment processes have been difficult to meet the increasing national drinking water standards, and more and more water plants have begun to use biological activated carbon advanced treatment process. Activated carbon itself has excellent physical adsorption performance. At the same time, activated carbon can provide a good carrier for microorganisms, so that it can play a role in biodegradation in the degradation of COD, ammonia nitrogen and other pollutants, and improve the effect of drinking water treatment. [0005] The biological activity in activated carbon is a key indicator to evaluate the biological treatment capacity of activated carbon. At present, the commonly used biological activity evaluation met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 李伟光俞洋边继踊孟利强
Owner HARBIN INST OF TECH
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