Method for producing biological organic matter by using threonine fermentation waste liquid

A technology of threonine fermentation liquid and fermentation waste liquid, which is applied in the field of bio-organic matter production, can solve problems such as difficult optimization of amino acid waste liquid components, limited utilization of amino acid waste liquid, and poor field effects, so as to achieve direct biological utilization and benefit Effects of plant growth, strong ion exchange capacity and adsorption capacity

Active Publication Date: 2021-09-17
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, due to the basic characteristics of the liquid, there are negative factors such as strong acidity, strong corrosiveness, heavy odor, difficulty in optimizing the composition of amino acid waste liquid, poor field effect, and even harmful to some crops, making amino acid waste liquid The use of organic fertilizers has been limited, and the fertilizers produced, whether solid waste or liquid fertilizers, are difficult to promote

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method of producing threonine by using a biological organic waste fermentation, comprising the steps of:

[0027] Step 1) engineering bacteria E. coli K12 △ dapA seed liquid (concentration of the seed liquid was 1 × 10 8 cfu / mL) of 8% in accordance with the access to the inoculum fermentor containing fermentation medium in a fermentation temperature of 30 ℃, 0.04MPa pressure tank, aeration rate 0.5 vvm, 100 rpm rotation speed, fermentation time 36h, then access Chlamydomonas reinhardtii (Chlamydomonas reinhardtii inoculation amount of 10 percent at a concentration of 1 × 10 5 cfu / mL), continued 36h fermentation, the fermentation was stopped, the fermentation broth was collected;

[0028] Component of the fermentation medium: glucose 20g / L, glycerol, 15g / L, corn steep liquor 15g / L, ammonium sulfate, 2g / L, potassium dihydrogen phosphate 0.2g / L, dipotassium hydrogen phosphate 0.2g / L, magnesium sulfate heptahydrate 0.1g / L, ferrous sulfate heptahydrate 0.01...

Embodiment 2

[0034] Fermentation process of the present invention, different factors influence on the yield and yield of acetic acid threonine:

[0035] Set Group:

[0036]Experimental group: Example 1;

[0037] Control Group 1: Chlamydomonas reinhardtii was not added, remaining the same as in Example 1;

[0038] Control 2: the same quality glycerol replace glucose, remaining the same as in Example 1;

[0039] Group 3: Chlamydomonas reinhardtii was not added, while replacing the same quality as glucose, glycerol, remaining the same as in Example 1.

[0040] The content of acetic acid was threonine and final fermentation respective groups shown in Table 1:

[0041] Table 1

[0042] Group Isine g / L G / L of acetic acid test group 127.9 0.7 Control group 1 103.5 12.6 Control group 2 115.7 4.9 Control group 3 97.1 14.8

[0043] Conclusion: the experimental group by C. reinhardtii auxiliary fermentation treatment, it is possible threonine fermentation broth ...

Embodiment 3

[0045] Bio-organic fertilizer test preparation of the present invention:

[0046] Tomato, for example, to detect fruit yield and quality.

[0047] Experimental group: spraying biological organic matter of the present invention;

[0048] Control group: equivalent amount of spray water.

[0049] Inner greenhouse site selection area; cells per acreage 10m × 10m, provided with three replications total of 6 test cells, randomized block arrangement. Tomato varieties Patent No. 1 powder, spraying liquid fertilizer per acre 100kg, the same as the other growing conditions. The result is shown in Table 2:

[0050] Table 2

[0051] Group Single fruit G Soluble solid% Soluble sugar% Irogate test group 168.9 4.73 4.03 0.36 Control group 162.4 4.55 3.89 0.41

[0052] Conclusion: As shown in Table 2 is not applied with the control group compared to the organic matter, organic matter can be enhanced in the experimental group of fruit weight, but also can improve t...

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PUM

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Abstract

The invention belongs to the field of biotechnology, and discloses a method for producing biological organic matter by using threonine fermentation waste liquid, which comprises the following steps: step 1) preparing threonine fermentation liquid, step 2) preparing protein powder, and step 3) preparing threonine Acid, step 4) collecting threonine fermentation waste liquid, step 5) preparing bio-organic matter. The method of the invention prepares bio-organic matter while preparing threonine, which can be used as liquid fertilizer, realizes turning waste into wealth, and kills two birds with one stone.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, particularly relates to a method of using a threonine fermentation production of biological waste organic matter. Background technique [0002] Threonine is an essential amino acid, an amino acid and a second limiting poultry feed pig feed third limiting amino acid, with lysine, methionine with a synthetic product widely used in feed, threonine becoming Effect the main limiting factor in the growth of livestock and poultry. Threonine mainly used as feed additive, which is a production step concentration, crystallization, bacterial protein extraction, and the difficulty of handling the mother liquor generated in the process, more costly, difficult to obtain a higher cost product. [0003] Currently wastewater fraction of the amino acid fermentation industry for the development of liquid fertilizer, as well as a small part of a process for spray granulation of solid organic fertilizer. However, to b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/08C12R1/19C05G3/80C05G3/90
CPCA23J1/001C05B7/00C12P13/08C05G3/80C05G3/90C05G5/20C05C9/00C05C11/00C05F11/02C05F11/08
Inventor 赵春晓徐太海张宗鑫罗玮
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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