Method for producing biological organic matter by using threonine fermentation waste liquid

Abstract

The invention belongs to the field of biotechnology, and discloses a method for producing biological organic matter by using threonine fermentation waste liquid, which comprises the following steps: step 1) preparing threonine fermentation liquid, step 2) preparing protein powder, and step 3) preparing threonine Acid, step 4) collecting threonine fermentation waste liquid, step 5) preparing bio-organic matter. The method of the invention prepares bio-organic matter while preparing threonine, which can be used as liquid fertilizer, realizes turning waste into wealth, and kills two birds with one stone.

Description

Technical field

[0001] The present invention belongs to the field of biotechnology, particularly relates to a method of using a threonine fermentation production of biological waste organic matter. Background technique

[0002] Threonine is an essential amino acid, an amino acid and a second limiting poultry feed pig feed third limiting amino acid, with lysine, methionine with a synthetic product widely used in feed, threonine becoming Effect the main limiting factor in the growth of livestock and poultry. Threonine mainly used as feed additive, which is a production step concentration, crystallization, bacterial protein extraction, and the difficulty of handling the mother liquor generated in the process, more costly, difficult to obtain a higher cost product.

[0003] Currently wastewater fraction of the amino acid fermentation industry for the development of liquid fertilizer, as well as a small part of a process for spray granulation of solid organic fertilizer. However, to b...

Examples

Embodiment 1

[0026] The method of producing threonine by using a biological organic waste fermentation, comprising the steps of:

[0027] Step 1) engineering bacteria E. coli K12 △ dapA seed liquid (concentration of the seed liquid was 1 × 10 8 cfu / mL) of 8% in accordance with the access to the inoculum fermentor containing fermentation medium in a fermentation temperature of 30 ℃, 0.04MPa pressure tank, aeration rate 0.5 vvm, 100 rpm rotation speed, fermentation time 36h, then access Chlamydomonas reinhardtii (Chlamydomonas reinhardtii inoculation amount of 10 percent at a concentration of 1 × 10 5 cfu / mL), continued 36h fermentation, the fermentation was stopped, the fermentation broth was collected;

[0028] Component of the fermentation medium: glucose 20g / L, glycerol, 15g / L, corn steep liquor 15g / L, ammonium sulfate, 2g / L, potassium dihydrogen phosphate 0.2g / L, dipotassium hydrogen phosphate 0.2g / L, magnesium sulfate heptahydrate 0.1g / L, ferrous sulfate heptahydrate 0.01...

Embodiment 2

[0034] Fermentation process of the present invention, different factors influence on the yield and yield of acetic acid threonine:

[0035] Set Group:

[0036]Experimental group: Example 1;

[0037] Control Group 1: Chlamydomonas reinhardtii was not added, remaining the same as in Example 1;

[0038] Control 2: the same quality glycerol replace glucose, remaining the same as in Example 1;

[0039] Group 3: Chlamydomonas reinhardtii was not added, while replacing the same quality as glucose, glycerol, remaining the same as in Example 1.

[0040] The content of acetic acid was threonine and final fermentation respective groups shown in Table 1:

[0041] Table 1

[0042] Group Isine g / L G / L of acetic acid test group 127.9 0.7 Control group 1 103.5 12.6 Control group 2 115.7 4.9 Control group 3 97.1 14.8

[0043] Conclusion: the experimental group by C. reinhardtii auxiliary fermentation treatment, it is possible threonine fermentation broth ...

Embodiment 3

[0045] Bio-organic fertilizer test preparation of the present invention:

[0046] Tomato, for example, to detect fruit yield and quality.

[0047] Experimental group: spraying biological organic matter of the present invention;

[0048] Control group: equivalent amount of spray water.

[0049] Inner greenhouse site selection area; cells per acreage 10m × 10m, provided with three replications total of 6 test cells, randomized block arrangement. Tomato varieties Patent No. 1 powder, spraying liquid fertilizer per acre 100kg, the same as the other growing conditions. The result is shown in Table 2:

[0050] Table 2

[0051] Group Single fruit G Soluble solid% Soluble sugar% Irogate test group 168.9 4.73 4.03 0.36 Control group 162.4 4.55 3.89 0.41

[0052] Conclusion: As shown in Table 2 is not applied with the control group compared to the organic matter, organic matter can be enhanced in the experimental group of fruit weight, but also can improve t...

Technical field

[0001] The present invention belongs to the field of biotechnology, particularly relates to a method of using a threonine fermentation production of biological waste organic matter. Background technique

[0002] Threonine is an essential amino acid, an amino acid and a second limiting poultry feed pig feed third limiting amino acid, with lysine, methionine with a synthetic product widely used in feed, threonine becoming Effect the main limiting factor in the growth of livestock and poultry. Threonine mainly used as feed additive, which is a production step concentration, crystallization, bacterial protein extraction, and the difficulty of handling the mother liquor generated in the process, more costly, difficult to obtain a higher cost product.

[0003] Currently wastewater fraction of the amino acid fermentation industry for the development of liquid fertilizer, as well as a small part of a process for spray granulation of solid organic fertilizer. However, to b...

Examples

Embodiment 1

[0026] The method of producing threonine by using a biological organic waste fermentation, comprising the steps of:

[0027] Step 1) engineering bacteria E. coli K12 △ dapA seed liquid (concentration of the seed liquid was 1 × 10 8 cfu / mL) of 8% in accordance with the access to the inoculum fermentor containing fermentation medium in a fermentation temperature of 30 ℃, 0.04MPa pressure tank, aeration rate 0.5 vvm, 100 rpm rotation speed, fermentation time 36h, then access Chlamydomonas reinhardtii (Chlamydomonas reinhardtii inoculation amount of 10 percent at a concentration of 1 × 10 5 cfu / mL), continued 36h fermentation, the fermentation was stopped, the fermentation broth was collected;

[0028] Component of the fermentation medium: glucose 20g / L, glycerol, 15g / L, corn steep liquor 15g / L, ammonium sulfate, 2g / L, potassium dihydrogen phosphate 0.2g / L, dipotassium hydrogen phosphate 0.2g / L, magnesium sulfate heptahydrate 0.1g / L, ferrous sulfate heptahydrate 0.01...

Embodiment 2

[0034] Fermentation process of the present invention, different factors influence on the yield and yield of acetic acid threonine:

[0035] Set Group:

[0036]Experimental group: Example 1;

[0037] Control Group 1: Chlamydomonas reinhardtii was not added, remaining the same as in Example 1;

[0038] Control 2: the same quality glycerol replace glucose, remaining the same as in Example 1;

[0039] Group 3: Chlamydomonas reinhardtii was not added, while replacing the same quality as glucose, glycerol, remaining the same as in Example 1.

[0040] The content of acetic acid was threonine and final fermentation respective groups shown in Table 1:

[0041] Table 1

[0042] Group Isine g / L G / L of acetic acid test group 127.9 0.7 Control group 1 103.5 12.6 Control group 2 115.7 4.9 Control group 3 97.1 14.8

[0043] Conclusion: the experimental group by C. reinhardtii auxiliary fermentation treatment, it is possible threonine fermentation broth ...

Embodiment 3

[0045] Bio-organic fertilizer test preparation of the present invention:

[0046] Tomato, for example, to detect fruit yield and quality.

[0047] Experimental group: spraying biological organic matter of the present invention;

[0048] Control group: equivalent amount of spray water.

[0049] Inner greenhouse site selection area; cells per acreage 10m × 10m, provided with three replications total of 6 test cells, randomized block arrangement. Tomato varieties Patent No. 1 powder, spraying liquid fertilizer per acre 100kg, the same as the other growing conditions. The result is shown in Table 2:

[0050] Table 2

[0051] Group Single fruit G Soluble solid% Soluble sugar% Irogate test group 168.9 4.73 4.03 0.36 Control group 162.4 4.55 3.89 0.41

[0052] Conclusion: As shown in Table 2 is not applied with the control group compared to the organic matter, organic matter can be enhanced in the experimental group of fruit weight, but also can improve t...