Method for creating zoysia japonica mutant by utilizing oxygen cold plasma mutagenesis

A technology of cold plasma and zoysia, applied in gardening methods, botanical equipment and methods, applications, etc., can solve the problems of slow growth of zoysia, long breeding cycle, hindering zoysia, etc., and achieve charged ion The effect of increasing the species and concentration of active particles, accelerating the breeding process, and broad application prospects

Active Publication Date: 2021-05-25
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the slow growth rate of zoysia seriously hinders the development of zoysia industry
At present, the cultivation of new varieties of Zoysias is mainly based on systematic breeding and hybrid breeding. The breeding cycle is long and the efficiency is low, which seriously hinders the breeding process of Zoysias. How to quickly select fast-growing and high-quality varieties has always been a scientific problem in Zoysias breeding
Zoysia has fewer fertile seeds, thicker seed coat, and thicker vegetative epidermis. It is difficult to achieve a good mutagenic effect by conventional methods

Method used

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  • Method for creating zoysia japonica mutant by utilizing oxygen cold plasma mutagenesis
  • Method for creating zoysia japonica mutant by utilizing oxygen cold plasma mutagenesis
  • Method for creating zoysia japonica mutant by utilizing oxygen cold plasma mutagenesis

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Experimental program
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Effect test

Embodiment 1

[0038] Get the young tiller buds ( figure 2 ), 15% H 2 o 2 Soak for 15 minutes, rinse with distilled water for 3 minutes, then soak in 50% alcohol for 8 minutes, rinse with distilled water for 10 minutes, sterilize and then inoculate into induction medium (medium formula: MS medium, add 2,4-D 6mg / L, L-proline 4g / L, 5-aminolevulinic acid 5mg / L, VB 1 2mg / L, sucrose 25g / L and phytogel 3g / L), cultivated in the dark at 25±1°C for 30d, and obtained Zoysia somatic tissue.

[0039] Take 5 granular somatic tissues with a diameter of 0.4 cm and place them in figure 1 In the quartz glass petri dish with an inner diameter of 6 cm in the cold plasma discharge device shown, place the petri dish between the two plates of radio frequency discharge, the distance from the electrode plates is 1 cm, close the reaction system, evacuate, and feed oxygen for 10 minutes. The pressure is 15Pa, the processing power is 650W, and the mutagenesis is 8min.

[0040] The cold plasma treated somatic tis...

Embodiment 2

[0047] Get the young and tender tiller buds of Zoysia rugosa with a length of 1 cm, and use 15% H 2 o 2 Soak for 15 minutes, rinse with distilled water for 5 minutes, then soak with 50% alcohol for 8 minutes, rinse with distilled water for 10 minutes, and inoculate the sterilized tiller buds into induction medium (MS medium, 2,4-D 8mg / L, L-proline 5g / L L, 5-aminolevulinic acid 7mg / L, VB 1 3mg / L, sugarcane 30g / L and plant gel 5g / L), cultured in the dark at 25±1°C for 30d to obtain Zoysia somatic cells.

[0048] Take 5 granular somatic tissues with a diameter of 0.7cm, place them in a quartz glass petri dish with a diameter of 6cm, place the petri dish between the two polar plates of radio frequency discharge, and the distance from the electrode plates is 2cm, and seal the reaction system. Vacuumize, feed oxygen for 12min, pressure is 30Pa, processing power is 750W, mutagenesis is 12min. The cold plasma-treated somatic tissue was inoculated into differentiation medium (MS med...

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Abstract

The invention provides a method for creating a zoysia japonica mutant by utilizing oxygen cold plasma mutagenesis, and relates to the technical field of mutation breeding. According to the method, the cold plasma technology is applied to the zoysia japonica somatic cell mutagenesis process, the zoysia japonica mutagenesis frequency is remarkably improved, the breeding cost is reduced, the zoysia japonica breeding process is accelerated, reference is provided for somatic cell mutation breeding of other plants, and the method has the advantages of being environmentally friendly, efficient, easy to operate and the like, and has a wide application prospect in the field of biological mutation breeding. By utilizing the method disclosed by the invention, the variation frequency is obviously improved compared with that of conventional mutation breeding and can reach 19.1%.

Description

technical field [0001] The invention belongs to the technical field of mutation breeding, and in particular relates to a method for creating mutants of zoysia grass by using oxygen cold plasma mutagenesis. Background technique [0002] Plants of the genus Zoysis Willd. are perennial herbaceous plants of the Poaceae Teffae subfamily. They are excellent warm-season lawn grasses and forages. They are widely used in landscaping and sports fields in warm-temperate to tropical regions at home and abroad. , soil fixation and slope protection and saline-alkali land greening lawn planting have important lawn use, water and soil conservation and feeding value. However, the slow growth rate of zoysia seriously hinders the development of zoysia industry. At present, the breeding of new varieties of Zoysias is mainly based on systematic breeding and hybrid breeding. The long breeding cycle and low efficiency have seriously hindered the breeding process of Zoysias. How to quickly select ...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H4/00
CPCA01H1/06A01H4/008A01H4/001
Inventor 李玲刘建秀宗俊勤王浩然郭海林郭爱桂陈静波李丹丹李建建
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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