A method for improving the survival rate of black poplar hybrid progeny seedling raising

A technology of survival rate and progeny, applied in the field of plant seedling raising, can solve the problems of long slow seedling period, easily damaged tissue culture seedlings, low seedling rate of hybrid progeny tissue culture seedlings, etc. The effect of planting survival rate
CN112868529BActive Publication Date: 2022-07-05INST OF FORESTRY CHINESE ACAD OF FORESTRY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF FORESTRY CHINESE ACAD OF FORESTRY
Publication Date
2022-07-05

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Abstract

The invention discloses a method for improving the survival rate of hybrid progeny seedlings of black poplar, comprising the following steps: 1) 30-40 days after pollination, cutting capsules from the base of fruit stalks for cleaning and disinfection, and using sterile filter paper to absorb moisture on the surface of the capsules; 2) Peel the pericarp along the longitudinal axis of the capsule carpel, and peel the flocs together with the seed embryos; 3) Inoculate the flocs together with the seed embryos on 1 / 2MS medium and cultivate to a seedling height of 4-6 cm or more, and there are 4-6 pieces True leaves; 4) The tissue culture seedlings were washed to remove the medium, transplanted into a non-woven container with a substrate, and moved into the field three weeks later. The method of the invention is simple, feasible, safe and reliable, and the survival rate reaches more than 95% after being transplanted into the field.
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Description

technical field

[0001] The invention belongs to the technical field of plant seedling raising, in particular to a method for improving the survival rate of black poplar hybrid progeny seedling raising. Background technique

[0002] Plant tissue culture, also known as in vitro culture, uses plant organs (such as roots, stems, leaves, shoot tips, flowers, fruits, etc.), tissues or cells and protoplasts in vitro, in sterile and suitable artificial medium and Under artificial conditions such as temperature, callus, adventitious buds, and adventitious roots can be induced, and finally a complete plant can be formed. Cross-breeding is the main means of cultivating new varieties of poplar. In order to improve the breeding efficiency and solve the problems of ear shedding and immature seed development in the late stage of indoor cutting and hydroponic hybridization, it is a feasible and effective method to culture hybrid embryos in the late stage of hydroponics. After the tissue c...

Claims

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