A method for improving the survival rate of black poplar hybrid progeny seedling raising

A technology of survival rate and progeny, applied in the field of plant seedling raising, can solve the problems of long slow seedling period, easily damaged tissue culture seedlings, low seedling rate of hybrid progeny tissue culture seedlings, etc. The effect of planting survival rate

Active Publication Date: 2022-07-05
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

It is not easy to solve the sterility, ventilation, and nutritional requirements for the root growth of tissue cultured seedlings at the same time when the above method is used for indoor hardening of seedlings. In addition, during the process from indoor hardening to field transplanting, the roots of tissue cultured seedlings are easily damaged. Longer, resulting in low seedling rate of hybrid progeny tissue culture seedlings

Method used

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  • A method for improving the survival rate of black poplar hybrid progeny seedling raising

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Effect test

Embodiment 1

[0025] The invention provides a seedling refining and transplanting method for poplar tissue culture seedlings, so as to improve the seedling generation rate of poplar hybrid breeding, which is specifically achieved through the following contents:

[0026] 1) After 30 days of pollination, use scissors to cut the capsule from the base of the fruit stalk, rinse the surface of the peel with tap water, drain the water, and put it into a sterile bottle. Sterilize by soaking in 75% alcohol for 6 minutes, then rinse with sterile water three times, put the material into a petri dish after rinsing, and dry the surface of the material with sterile filter paper.

[0027] 2) Gently press with a blade along the longitudinal axis of the carpel, peel off the pericarp, gently peel off the flocculation together with the seed embryo, evenly inoculate it on 1 / 2MS medium, and press lightly with an inoculation tool to fully contact the medium, Conducive to emergence and growth. The culture enviro...

Embodiment 2

[0033] The invention provides a method for hardening and transplanting the hybrid embryo tissue culture seedlings of Zhongqian No. 3 × Juba poplar, Danhong poplar × Zhonghan 17, Zhongzhu No. 6 × Juba poplar and I-69 × Juba poplar. Improve the seedling rate of hybrid breeding seeds, specifically through the following content:

[0034] 1) The pistils of Zhongqian 3, Zhongzhu 6 and I-69 poplars were pollinated with the pollen of Juba Populus, and the pistils of Danhong Poplar were pollinated with the pollen of Zhonghan 17 respectively. After 30 days, they were cut from the base of the fruit stalk with scissors. Under the capsule, rinse the surface of the peel with tap water, drain the water, and put it into a sterile bottle. Sterilize by soaking in 75% alcohol for 6 minutes, then rinse with sterile water three times, put the material into a petri dish after rinsing, and dry the surface of the material with sterile filter paper.

[0035] 2) Gently press with a blade along the lon...

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Abstract

The invention discloses a method for improving the survival rate of hybrid progeny seedlings of black poplar, comprising the following steps: 1) 30-40 days after pollination, cutting capsules from the base of fruit stalks for cleaning and disinfection, and using sterile filter paper to absorb moisture on the surface of the capsules; 2) Peel the pericarp along the longitudinal axis of the capsule carpel, and peel the flocs together with the seed embryos; 3) Inoculate the flocs together with the seed embryos on 1 / 2MS medium and cultivate to a seedling height of 4-6 cm or more, and there are 4-6 pieces True leaves; 4) The tissue culture seedlings were washed to remove the medium, transplanted into a non-woven container with a substrate, and moved into the field three weeks later. The method of the invention is simple, feasible, safe and reliable, and the survival rate reaches more than 95% after being transplanted into the field.

Description

technical field [0001] The invention belongs to the technical field of plant seedling raising, in particular to a method for improving the survival rate of black poplar hybrid progeny seedling raising. Background technique [0002] Plant tissue culture, also known as in vitro culture, uses plant organs (such as roots, stems, leaves, shoot tips, flowers, fruits, etc.), tissues or cells and protoplasts in vitro, in sterile and suitable artificial medium and Under artificial conditions such as temperature, callus, adventitious buds, and adventitious roots can be induced, and finally a complete plant can be formed. Cross-breeding is the main means of cultivating new varieties of poplar. In order to improve the breeding efficiency and solve the problems of ear shedding and immature seed development in the late stage of indoor cutting and hydroponic hybridization, it is a feasible and effective method to culture hybrid embryos in the late stage of hydroponics. After the tissue c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G9/029A01G17/00A01G24/10A01G24/12A01G24/15A01G24/28A01G31/00
CPCA01H4/008A01H4/001A01G31/00A01G24/28A01G24/15A01G24/10A01G17/005A01G9/0291A01G24/12Y02P60/21
Inventor 丁昌俊董玉峰张伟溪苏晓华李善文王延平黄秦军王雷
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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