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A method for extracting 1,3-propanediol in fermentation broth with alcohol mixed solvent

A mixed solvent and propylene glycol technology, applied in the field of biological engineering, can solve the problems of increasing the complexity of the separation process, energy consumption, and large loss of extractant, and achieve the effects of reducing losses and reducing separation pressure.

Active Publication Date: 2022-04-29
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although multi-stage extraction can recover most of the 1,3-propanediol, but at the same time, more water enters the extraction phase, and the loss of extractant is also greater, which increases the complexity and energy consumption of the separation process

Method used

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  • A method for extracting 1,3-propanediol in fermentation broth with alcohol mixed solvent
  • A method for extracting 1,3-propanediol in fermentation broth with alcohol mixed solvent
  • A method for extracting 1,3-propanediol in fermentation broth with alcohol mixed solvent

Examples

Experimental program
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Effect test

preparation example Construction

[0026](1) Preparation of 1,3-propanediol fermentation broth: 1,3-propanediol fermentation broth is obtained by batch fed-batch fermentation by Clostridium butyricum, fermentation culture conditions: inoculum size 10% (v / v), 1h before inoculation Nitrogen was blown, the fermentation temperature was 37°C, the fermentation stirring speed was 250r / min, and 5mol / L NaOH solution was used to adjust the pH to maintain neutrality during the fermentation process. The initial glycerol concentration was 80g / L, and the feed was fed in two pulses. When the glycerin concentration dropped below 15g / L, the feed was fed to 80g / L. Fermentation was terminated when glycerol was no longer consumed. Clostridium butyricum is a commercially available Clostridium butyricum commonly used in the art for fermentative production of 1,3-propanediol.

[0027] (2) Preparation of 1,3-propanediol supernatant: use a conventional centrifuge or a membrane separation system to remove bacteria from the 1,3-propaned...

Embodiment 1

[0036] Example 1 The extraction effect of two kinds of alcohol mixed solvents based on undecyl alcohol composition

[0037] The materials used in this example are simulated solutions prepared with n-propanol, isopropanol, 1,2-propanediol, and 1,3-propanediol standard products respectively, with a concentration of 100 g / L. The extractant is prepared by mixing undecanol with dodecanol, isotridecanol, tetradecyl alcohol, pentadecyl alcohol and hexadecanol at 60°C at a ratio of 1:1 (mol / mol). At 60°C, mix 10mL of extractant with 10mL of n-propanol, isopropanol, 1,2-propanediol, and 1,3-propanediol simulation solution, shake well for 1min, and let stand for 5h. The content was determined by gas chromatography, and the yield was calculated. The results are shown in Table 1.

[0038] Table 1. Extraction results of n-propanol, isopropanol, 1,2-propanediol and 1,3-propanediol

[0039]

[0040] For extractants with different components, the greater the total number of C atoms of th...

Embodiment 2

[0041] Embodiment 2 Two kinds of alcohols mixed solvents are to the extraction effect of 1,3-propanediol

[0042] The material used in this example is a simulated solution prepared with a 1,3-propanediol standard product, with a concentration of 100 g / L. The extractant is a mixed alcohol solvent of four series: (1) n-octanol is mixed with n-decyl alcohol, undecyl alcohol, dodecyl alcohol, isotridecanol, and myristyl alcohol respectively; Mixture of monoalcohol, lauryl alcohol, isotridecanol, tetradecyl alcohol and pentadecyl alcohol; (3) undecyl alcohol mixed with dodecyl alcohol, isotridecanol, tetradecyl alcohol, pentadecyl alcohol and cetyl alcohol respectively (4) Dodecyl alcohol is mixed with isotridecanol, tetradecyl alcohol, pentadecyl alcohol, cetyl alcohol, and stearyl alcohol respectively. The mixing condition is 60°C, and the two alcohols are mixed uniformly at a ratio of 1:1 (mol / mol). At 60°C, take 10mL of extractant and 10mL of 1,3-propanediol simulated solutio...

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Abstract

The invention discloses a method for extracting 1,3-propanediol in a fermentation liquid with an alcohol mixed solvent. Including: the step of extracting the microbial fermentation liquid containing 1,3-propanediol with an alcohol mixed solvent, wherein the alcohol mixed solvent is composed of two or more alcohol substances, and at least two alcohol substances The difference in carbon number is 3. The present invention adopts mixed alcohols with specific C numbers, so that the extraction synergistic effect of alcohol mixed solvents can be optimized, and 1,3-propanediol can be extracted under the condition that the extractant has strong hydrophobicity, reducing the multi-stage extraction When the loss of extractant, while removing most of the water-soluble impurities in the fermentation broth, reduce the pressure of subsequent refining and separation.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a separation technology of microbial fermentation products, in particular to a method for separating and extracting 1,3-propanediol in a fermentation liquid. Background technique [0002] 1,3-propanediol (1,3-PDO) is a widely used chemical raw material, which can be used to manufacture high-performance polyester fiber-polytrimethylene terephthalate (PTT). In addition, it can also be used Used in the manufacture of hot melt adhesives, powder coatings, antifreeze agents, packaging materials and organic synthesis intermediates. 1,3-propanediol can be produced by chemical synthesis (ethylene oxide method, acrolein method, etc.) or biotransformation. The large-scale production and application of 1,3-propanediol are limited by the chemical synthesis method because the reaction requires high temperature and high pressure, and the intermediate product is toxic. The biological method can pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C29/86C07C31/20
CPCC07C29/86C07C31/205
Inventor 修志龙隋温博王晓丽孙亚琴
Owner DALIAN UNIV OF TECH
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