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Lactobacillus plantarum for preventing and/or treating periodontitis as well as culture, preparation and application of lactobacillus plantarum

A technology of Lactobacillus plantarum and culture, applied in the field of microorganisms, can solve the problems of microbial resistance, stomach discomfort of patients, and increase of adverse drug reactions.

Active Publication Date: 2021-06-22
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, clinical investigations have found that long-term use of metronidazole, minol hydrochloride and other drugs can cause stomach discomfort in some patients and increase the probability of adverse drug reactions, leading to the emergence of drug resistance in the microorganisms in the human body and destroying the normal oral cavity. micro-ecological environment

Method used

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  • Lactobacillus plantarum for preventing and/or treating periodontitis as well as culture, preparation and application of lactobacillus plantarum
  • Lactobacillus plantarum for preventing and/or treating periodontitis as well as culture, preparation and application of lactobacillus plantarum
  • Lactobacillus plantarum for preventing and/or treating periodontitis as well as culture, preparation and application of lactobacillus plantarum

Examples

Experimental program
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Effect test

preparation example Construction

[0075] The preparation method of the bacterial suspension involved in the following examples is as follows:

[0076] Fusobacterium nucleatum suspension: Inoculate the Fusobacterium nucleatum cells into the BHI medium with an inoculum amount accounting for 2% of the total volume of the BHI medium, and adjust the concentration of the bacterial solution with BHI medium after anaerobic cultivation at 37°C for 48 hours 1×10 9 CFU / mL.

[0077] Porphyromonas gingivalis suspension: inoculate Porphyromonas gingivalis cells into BHI medium at an inoculum amount accounting for 2% of the total volume of BHI medium, and inoculate in BHI medium after anaerobic cultivation at 37°C for 48 hours Adjust the bacterial concentration to 1×10 9 CFU / mL.

[0078] Intermediate Prevotella bacteria suspension: Inoculate the Prevotella intermediate bacteria into the BHI medium with an inoculum amount accounting for 2% of the total volume of the BHI medium, and adjust the concentration of the bacterial...

Embodiment 1

[0083] Screening and strain identification of embodiment 1 lactobacillus plantarum

[0084] 1. Screening

[0085] Take kimchi water from Chongqing area as the sample, after pretreatment, store the sample in 20% glycerol in a -80°C refrigerator, take it out and thaw it, mix the sample evenly, add 0.5mL sample to 4.5mL 9g / L physiological Perform gradient dilution with saline, select a suitable gradient dilution and spread 100 μL on the MRS medium containing 20 g / L agar, and incubate upside down at 37°C for 48 hours. At this time, white colonies can be observed on the plate. Use an inoculation loop to pick some typical colonies of Lactobacillus plantarum, streak and purify them three times on the MRS medium containing 20g / L agar, pick several purified single colonies and transfer them to the MRS medium for enrichment, 30% glycerol Preserved to obtain bacterial strain CCFM1137 and bacterial strains 1-14 screened by the same method at the same time; wherein, the typical colonies o...

Embodiment 2

[0088] The influence of embodiment 2 lactobacillus plantarum on the amount of mixed bacteria biofilm

[0089] The experiment was divided into two groups, respectively Lactobacillus plantarum-mediated group and blank control group, and each group was repeated 6 times;

[0090] Wherein, the Lactobacillus plantarum-mediated group is processed according to the following method:

[0091] (1) Lactobacillus plantarum CCFM1137, Lactobacillus plantarum CCFM8724, and Lactobacillus plantarum 1-14 were inoculated into MRS medium at an inoculum amount accounting for 2% of the total volume of the MRS medium, and cultured at 37°C for 24 hours to obtain For the culture solution, the culture solution was centrifuged at 8000r / min and 4°C for 5min to obtain the supernatant, and the supernatant was filtered through a 0.22μm sterile filter membrane to obtain a bacterial concentration of 10 9 CFU / mL supernatant of Lactobacillus plantarum CCFM1137, Lactobacillus plantarum CCFM8724, Lactobacillus pl...

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Abstract

The invention relates to the technical field of microorganisms, and discloses lactobacillus plantarum for preventing and / or treating periodontitis as well as a culture, preparation and application of the lactobacillus plantarum. The name of the lactobacillus plantarum is Lactobacillus plantarum CCFM1137, the lactobacillus plantarum is preserved in Guangdong Microbial Culture Collection Center on the 5th floor of the Building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou on August 1, 2020, and the preservation number is GDMCC No: 61114. The lactobacillus plantarum can be used for inhibiting the formation of a mixed bacterium biological membrane of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum; the content of inflammatory factors in a periodontitis cell model can be reduced; the expression quantity of transmembrane transport protein in a periodontitis cell model can be increased; and the lactobacillus plantarumcan be used for preventing and / or treating oral diseases and inhibiting prevotella intermedia, porphyromonas gingivalis and / or fusobacterium nucleatum.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to plantaractobacillus for preventing and / or treating periodontitis, its culture and its preparation and application. Background technique [0002] Periodontitis is an infectious inflammatory disease of the supporting tissues of the oral cavity. Periodontitis is usually accompanied by symptoms such as bad breath, swollen and painful gums, loose teeth, and masticatory dysfunction. It is also the leading cause of tooth loss in adults. More and more studies have shown that periodontitis pathogenic bacteria such as Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia are closely related to a variety of systemic diseases, such as diabetes, Alzheimer's disease, and colon cancer. [0003] Periodontitis is a multifactorial disease. In addition to plaque microorganisms and their products, which are essential initiating factors, the occurrence of pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61K36/539A61P1/02A61P31/04A61K8/99A61K8/9789A61Q11/00A61Q17/00A23L33/105A23L33/135C12R1/25
CPCC12N1/20A61K35/747A61K36/355A61K36/539A61P1/02A61P31/04A61K8/99A61K8/9789A61Q11/00A61Q17/005A23L33/105A23L33/135A23V2002/00A23V2400/169A61K2300/00A23V2200/312A23V2200/3204
Inventor 郑彦懿胡明华张秋香徐晚晴陆文伟
Owner INFINITUS (CHINA) CO LTD
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