Hydrogel drug-loaded coating for treatment in oxidative stress environment and preparation method of hydrogel drug-loaded coating

A technology of oxidative stress and drug-loaded coatings, applied in coatings, medical science, surgery, etc., can solve the problems of unresponsive release of drugs, unfavorable cell growth, and affecting physiological functions, etc., to inhibit the growth of macrophages, The effect of avoiding intravascular restenosis and promoting the repair of myocardial disease

Active Publication Date: 2021-06-29
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The current research on the problem of interventional drug-loaded coatings for the treatment of cardiovascular diseases has the following deficiencies: (1) The existing interventional materials mainly use spraying technology, and the bonding force between the coating and the stent is poor. After implantation, the coating It is easy to fall off and has poor adhesion, which is not conducive to cell growth; (2) The coating is mostly made of polymer materials, and the degradation products have certain toxic and side effects. For example, the degradation products of polylactic acid have certain acidi

Method used

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  • Hydrogel drug-loaded coating for treatment in oxidative stress environment and preparation method of hydrogel drug-loaded coating
  • Hydrogel drug-loaded coating for treatment in oxidative stress environment and preparation method of hydrogel drug-loaded coating
  • Hydrogel drug-loaded coating for treatment in oxidative stress environment and preparation method of hydrogel drug-loaded coating

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Experimental program
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Effect test

Embodiment 1

[0049] The invention provides a method for preparing a hydrogel drug-loaded coating for treatment in an oxidative stress environment, comprising the following steps:

[0050] S1 Catechol Modified Hyaluronic Acid

[0051] S1.1 Add 976mg MES and 1g hyaluronic acid with a molecular weight of 120wDa to RO water in turn, adjust the pH value of the solution to 5.5 after dissolution;

[0052] S1.2 Add 388mg of EDC to RO water, stir evenly, add to the solution obtained in step S1.1, then add 288mg of NHS, and keep stirring;

[0053] S1.3 After continuously stirring the solution obtained in step S1.2 for 30 minutes, add 471 mg of dopamine, and adjust the pH value to 5.5, and continue stirring for 4 hours;

[0054] S1.4 Dialyze the solution obtained in step S1.3 in RO water for 48 hours, freeze-dry and store in vacuum to obtain modified hyaluronic acid;

[0055] Cross-linking of S2-modified hyaluronic acid and cystamine

[0056] S2.1 Dissolve 1g of modified hyaluronic acid in 95mL of...

Embodiment 2

[0064] The invention provides a method for preparing a hydrogel drug-loaded coating for treatment in an oxidative stress environment, comprising the following steps:

[0065] S1 Catechol Modified Hyaluronic Acid

[0066] S1.1 Add 1g MES and 1g hyaluronic acid with a molecular weight of 120wDa to RO water in turn, adjust the pH value of the solution to 5.6 after dissolving;

[0067] S1.2 Add 400mg of EDC to RO water, stir evenly, add to the solution obtained in step S1.1, then add 300mg of NHS, and keep stirring;

[0068] S1.3 After continuously stirring the solution obtained in step S1.2 for 35 minutes, add 500 mg of dopamine, and adjust the pH value to 5.6, and continue stirring for 4 hours;

[0069] S1.4 Dialyze the solution obtained in step S1.3 in RO water for 48 hours, freeze-dry and store in vacuum to obtain modified hyaluronic acid;

[0070] Cross-linking of S2-modified hyaluronic acid and cystamine

[0071] S2.1 Dissolve 500mg of modified hyaluronic acid in 95mL of ...

Embodiment 3

[0079] The invention provides a method for preparing a hydrogel drug-loaded coating for treatment in an oxidative stress environment, comprising the following steps:

[0080] S1 Catechol Modified Hyaluronic Acid

[0081] S1.1 Add 900mg MES and 900mg hyaluronic acid with a molecular weight of 120wDa to RO water in turn, adjust the pH value of the solution to 5.5 after dissolving;

[0082] S1.2 Add 500mg of EDC to RO water, stir evenly, add to the solution obtained in step S1.1, then add 350mg of NHS, and keep stirring;

[0083] S1.3 After continuously stirring the solution obtained in step S1.2 for 35 minutes, add 550 mg of dopamine, and adjust the pH value to 5.5, and continue stirring for 5 hours;

[0084] S1.4 Dialyze the solution obtained in step S1.3 in water for 45 hours, freeze-dry and store in vacuum to obtain modified hyaluronic acid;

[0085] Cross-linking of S2-modified hyaluronic acid and cystamine

[0086] S2.1 Dissolve 1g of modified hyaluronic acid in 95mL of ...

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Abstract

The invention discloses a hydrogel drug-loaded coating for treatment in an oxidative stress environment and a preparation method of the hydrogel drug-loaded coating. The preparation method comprises the following steps: S1, modifying hyaluronic acid with catechol; S2, crosslinking of the modified hyaluronic acid and cystamine; and S3, preparing the hydrogel drug-loaded coating. Hydrogel containing S-S bonds can be formed by modifying and cross-linking hyaluronic acid, and the hydrogel and allicin are sequentially sprayed on a substrate on which polydopamine is deposited, so that the hydrogel drug-loaded coating for the oxidative stress environment can be prepared. The coating can realize intelligent and accurate drug release aiming at heart diseases caused by various oxidative stress such as myocardial infarction, pyotoxic cardiomyopathy, atherosclerosis and the like in specific pathological environments, so that inhibition on the oxidative stress and regulation and control on subsequent inflammatory response of the oxidative stress are realized.

Description

technical field [0001] The invention relates to the technical field of preparation of hydrogel carriers, in particular to a drug-loaded hydrogel coating for treatment in an oxidative stress environment and a preparation method thereof. Background technique [0002] According to the statistics of the World Health Organization, cardiovascular disease has become one of the main causes of abnormal human death in the world, and it is the highest morbidity and mortality rate among all diseases in my country, and it is higher than other diseases such as tumors. It poses a huge threat to human health and life. Among them, coronary heart disease (coronary heart disease) is a class of vascular-derived diseases with extremely high hazards caused by atherosclerosis. Atherosclerosis generally refers to lesions that occur in the intima of large and medium arteries, and is a disease caused by abnormal deposition of lipids in the plasma in the intima of the vessel wall. Atherosclerosis is...

Claims

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Application Information

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IPC IPC(8): A61L31/10A61L31/08A61L31/14A61L31/16C08B37/08
CPCA61L31/08A61L31/10A61L31/145A61L31/16A61L2300/216A61L2300/404A61L2300/416A61L2300/422A61L2420/06C08B37/0072C08L5/08
Inventor 赵安莎韩啸路槟阳丁艺蕾杨苹
Owner SOUTHWEST JIAOTONG UNIV
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