Application of glucosinolate in preparation of product for preventing and treating narrowleaf knotweed herb and insect-resistant breeding

A glucosinolate, pewter technology, applied in applications, pesticides, gardening tools/equipment, etc., can solve the problem that the compound has not been further verified.

Active Publication Date: 2021-07-06
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of glucosinolate in preparation of product for preventing and treating narrowleaf knotweed herb and insect-resistant breeding
  • Application of glucosinolate in preparation of product for preventing and treating narrowleaf knotweed herb and insect-resistant breeding
  • Application of glucosinolate in preparation of product for preventing and treating narrowleaf knotweed herb and insect-resistant breeding

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 The identification method of the compound associated with the anti-white wax narrow gelatinous trait of Ash tree comprises the following steps:

[0032] 1) Metabolomic analysis of insect-resistant and insect-susceptible ash tree species

[0033] According to the identification data of insect resistance for many years in the early stage, it was found that the native species of Chinese white wax species, Huaquliu, was immune or highly resistant to white wax, while the foreign white wax imported from North America had a high rate of insect sensitivity (such as figure 1 shown). Therefore, Aspergillus japonica and P. japonica were selected as insect-resistant and susceptible varieties, respectively, and metabolomics analysis was performed to screen candidate insect-resistant compounds.

[0034] In the ash tree area of ​​Jingyuetan National Forest Park, Changchun City, Jilin Province, for the ash tree seedlings with a DBH of about 2.5cm and a tree height of abou...

Embodiment 2

[0044] Example 2 The application of sinapin glucoside in the preparation of products for the prevention and treatment of white wax narrow gelatin

[0045] Using DMSO as a solvent, prepare a solution of sinapin glucoside, the concentration of which is 0.1 times and 1 times the content of the insect-resistant variety Aspergillus fragrans, namely 4.2mg / g and 42mg / g. The sinapin glucoside is a purchased pure product (purity ≥ 98%).

[0046] Collect wood with insects from the wild, separate the larvae, raise them until they pupate, lay eggs, and then hatch the larvae. Preparation of white wax narrow gelding semi-artificial diet. The specific operation is as follows: take the bark of the branches of the ash tree, freeze the bark thoroughly with liquid nitrogen, and pulverize the bark at a low temperature. Add agar and yeast powder to the water, and heat it in the microwave oven until it is completely dissolved, then add sorbic acid, methyl p-hydroxybenzoate and ascorbic acid in se...

Embodiment 3

[0048] The application of embodiment 3 sinapin glucoside in the assisted breeding of resisting ash-resistant white wax narrow gelding ash

[0049] In the process of breeding insect-resistant varieties of ash tree, in order to ensure the validity and accuracy of the test, the insect resistance test usually requires observation of at least one insect generation, which takes a long time and is time-consuming and laborious. The content of the compound in the plant is determined by the genotype. Through metabolomics analysis, correlation analysis and insect resistance verification, the present invention finds that the content of sinapin glucoside in the bark of the ash tree is positively correlated with the resistance of the ash cinnamon. Therefore, Sinsapardin glucoside can be used as a metabolic marker to predict the resistance level of ash tree to ash narrow gelatin.

[0050] Utilize sinapin glucoside to carry out the implementation process of the assisted breeding of white wax ...

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Abstract

The invention discloses an application of glucosinolate in preparation of a product for preventing and treating narrowleaf knotweed herb, and the glucosinolate is used as a main component of a pesticide and can be used for poisoning and killing narrowleaf knotweed herb larvae. The invention further discloses an application of the glucosinolate in the auxiliary breeding of fraxinus chinensis resistant to narrowleaf knotweed herb, the glucosinolate is used as a metabolic marker in the auxiliary breeding of the fraxinus chinensis resistant to narrowleaf knotweed herb, the auxiliary selection of the glucosinolate is effectively combined with a conventional breeding technology, the insect-resistant performance of the fraxinus chinensis can be judged in an early stage, the breeding time is shortened, the breeding speed is increased, and the insect-resistant breeding efficiency of the fraxinus chinensis is improved. The invention provides a new direction and method for insect-resistant breeding of fraxinus chinensis and effective control of narrowleaf speedwell, and has important practical significance.

Description

technical field [0001] The invention relates to the technical field of forest tree insect-resistant breeding, and relates to the application of sinapin glucoside in the preparation of white wax narrow jelly products and insect-resistant breeding. Background technique [0002] White wax (scientific name: Fraxinus chinensis) is a general name for plants of the genus Fraxinus in the Oleaceae family, also known as ash. It is named the white wax tree because the white wax worms are stocked on the tree, and it is also a sand-fixing tree species. There are about 70 species in this genus, which are mainly distributed in the temperate zone of the northern hemisphere, and very few extend southward to the tropics. There are more than 20 species in China, and the most widely distributed is the ash tree. The wood is tough and resistant to water and humidity, and can be used to make furniture, farm tools, plywood, etc.; Therefore, the ash tree plays an important role in the economic con...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P7/04A01G7/06A01G13/00
CPCA01N43/16A01G7/06A01G13/00
Inventor 曲良建应玥谢丹洁
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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