Application of glucosinolate in preparation of product for preventing and treating narrowleaf knotweed herb and insect-resistant breeding
A glucosinolate, pewter technology, applied in applications, pesticides, gardening tools/equipment, etc., can solve the problem that the compound has not been further verified.
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Embodiment 1
[0031] Embodiment 1 The identification method of the compound associated with the anti-white wax narrow gelatinous trait of Ash tree comprises the following steps:
[0032] 1) Metabolomic analysis of insect-resistant and insect-susceptible ash tree species
[0033] According to the identification data of insect resistance for many years in the early stage, it was found that the native species of Chinese white wax species, Huaquliu, was immune or highly resistant to white wax, while the foreign white wax imported from North America had a high rate of insect sensitivity (such as figure 1 shown). Therefore, Aspergillus japonica and P. japonica were selected as insect-resistant and susceptible varieties, respectively, and metabolomics analysis was performed to screen candidate insect-resistant compounds.
[0034] In the ash tree area of Jingyuetan National Forest Park, Changchun City, Jilin Province, for the ash tree seedlings with a DBH of about 2.5cm and a tree height of abou...
Embodiment 2
[0044] Example 2 The application of sinapin glucoside in the preparation of products for the prevention and treatment of white wax narrow gelatin
[0045] Using DMSO as a solvent, prepare a solution of sinapin glucoside, the concentration of which is 0.1 times and 1 times the content of the insect-resistant variety Aspergillus fragrans, namely 4.2mg / g and 42mg / g. The sinapin glucoside is a purchased pure product (purity ≥ 98%).
[0046] Collect wood with insects from the wild, separate the larvae, raise them until they pupate, lay eggs, and then hatch the larvae. Preparation of white wax narrow gelding semi-artificial diet. The specific operation is as follows: take the bark of the branches of the ash tree, freeze the bark thoroughly with liquid nitrogen, and pulverize the bark at a low temperature. Add agar and yeast powder to the water, and heat it in the microwave oven until it is completely dissolved, then add sorbic acid, methyl p-hydroxybenzoate and ascorbic acid in se...
Embodiment 3
[0048] The application of embodiment 3 sinapin glucoside in the assisted breeding of resisting ash-resistant white wax narrow gelding ash
[0049] In the process of breeding insect-resistant varieties of ash tree, in order to ensure the validity and accuracy of the test, the insect resistance test usually requires observation of at least one insect generation, which takes a long time and is time-consuming and laborious. The content of the compound in the plant is determined by the genotype. Through metabolomics analysis, correlation analysis and insect resistance verification, the present invention finds that the content of sinapin glucoside in the bark of the ash tree is positively correlated with the resistance of the ash cinnamon. Therefore, Sinsapardin glucoside can be used as a metabolic marker to predict the resistance level of ash tree to ash narrow gelatin.
[0050] Utilize sinapin glucoside to carry out the implementation process of the assisted breeding of white wax ...
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