Plasma coagulation cell block method and clinical application thereof

Abstract

The invention relates to the technical field of clinical medical pathological diagnosis, and particularly discloses a plasma coagulation cell block method and clinical application thereof. The plasma coagulation cell block method comprises the following steps: S1, taking 10mL of a specimen test solution (such as hydrothorax and ascites), adding the specimen test solution into a centrifugal tube, centrifuging at the rotating speed of 2800-3200r / min for 3-5min, and discarding supernatant liquid to obtain cell sediments; s2, adding plasma into the cell sediments in the S1, uniformly stirring and mixing, adding a thrombin reagent, immediately stirring for 3-5 seconds, and keeping the temperature in a water bath box at 35-40 DEG C for 3-5 minutes to obtain cell clots; and s3, pouring the cell clots in the S2 into a formalin reagent for fixation, and then preparing into a cell paraffin block; the clinical application of the plasma coagulated cell block method is as follows: 1, after the cell paraffin block is sliced and dyed, pathological film reading diagnosis can be carried out; 2, the cell paraffin block can be used for immunohistochemistry to help pathological diagnosis; 3, the cell paraffin block can be used for gene detection to guide targeted therapy; and 4, the cell paraffin block can be kept for a long time and can be used for other scientific researches.

Description

technical field [0001] The application relates to the technical field of clinical medicine pathological diagnosis, more specifically, it relates to a kind of plasma coagulation cell block method and its clinical application. Background technique [0002] Pathological examination is a pathomorphological method used to examine pathological changes in body organs, tissues or cells. In order to explore the disease process of organs, tissues or cells, some pathological morphological examination method can be used to check their lesions, discuss the cause of lesions, pathogenesis, and development process of lesions, and finally make a pathological diagnosis . The examination method of pathomorphology, first of all, the general pathological changes of the specimens are examined, and then the lesion tissue of a certain size is cut out, and pathological sections are made by pathohistological methods, and further examination is carried out with a microscope. [0003] Cytopathologica...

AI Technical Summary

Problems solved by technology

[0003] Cytopathological examination is an important part of cytopathology. Due to the difficulty of obtaining living tissue, the clinical desire to confirm the diagnosis by cytology is very strong. However, cytology has its inherent limitations in the convenience of diagnosing malignant tumors, that is, it is difficult to see tumors. The biological characteristics of invasiveness, and invasiveness is the fundamental characteristic that distinguishes malignant tumors from benign tumors, and is also the key to diagnosis

[0004] However, it is not easy to perform smear examination on cells. Professor Ma Zhengzhong pointed out that "many smears that are difficult to diagnose are actually caused by poor quality of smears", and proposed the standard of good quality smears, emphasizing that smears The cell structure in the slice must be clear, and structures such as nuclear membrane, chromatin and nucleolus must be clear