Composition for removing phospholipids and cell debris and method for removing phospholipids and cell debris from biological tissue

A technology for biological tissue and cell debris, applied to the composition for removing phospholipids and cell debris, and the field of removing phospholipids and cell debris on biological tissues, which can solve the problems of functional group exposure, calcification, damage, etc.

Active Publication Date: 2022-06-21
SHANGHAI NEWMED MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the valve is damaged during multiple switching processes, which leads to the exposure of functional groups and calcification. However, there is currently no good method for removing phospholipids, cells and cell debris residues in the pericardium.

Method used

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  • Composition for removing phospholipids and cell debris and method for removing phospholipids and cell debris from biological tissue
  • Composition for removing phospholipids and cell debris and method for removing phospholipids and cell debris from biological tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Put 6*6cm pig pericardium into 360ml of the composition for removing phospholipids and cell debris (the composition is 50wt% ethanol, 40wt% propylene glycol methyl ether, 0.1wt% sodium deoxycholate, HEPES buffer is the remaining Quantity), the treatment temperature was 40 °C, and the treatment time was 24 h to obtain the crudely treated biological tissue; put the crudely treated biological tissue into 360 ml of fixative solution at 20 °C (the fixative solution was 1wt% glutaraldehyde , PBS buffer is the balance) for 7 days to obtain the fixed biological tissue. Put the fixed biological tissue into 360ml of sterilization solution (the sterilization solution is 2wt% glutaraldehyde, 3wt% formaldehyde, 15wt% isopropanol, PBS buffer is the balance) for sterilization, and the sterilization temperature is 50° C., and the sterilization time is 24 h, to obtain the biological tissue from which the phospholipids and cell debris have been removed, and subject the biological tissue ...

Embodiment 10

[0069] The difference between Example 10 and Example 5 is that the types of alcohol ethers are different, see Table 1 for details.

Embodiment 11

[0070] The difference between Example 11 and Example 4 is that the type of alcohol is different, see Table 1 for details.

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Abstract

Compositions for removing phospholipids and cell debris and methods for removing phospholipids and cell debris from biological tissues. The application discloses a composition for removing phospholipids and cell debris, including alcohols, alcohol ethers and surfactants, wherein alcohols are 50-90wt%, preferably 60-80wt%, and alcohol ethers are 5-90wt%. 40wt%, preferably 10-20wt%, surfactant is 0.1-5wt%, preferably 1-3wt%. The present application also provides a method for removing phospholipids and cell debris from biological tissues. The biological tissue is treated with the composition described in this application, and the treated biological tissue has an excellent anti-calcification effect.

Description

technical field [0001] The present application relates to the field of biological tissues, and more particularly to a composition for removing phospholipids and cell debris and a method for removing phospholipids and cell debris from biological tissues. Background technique [0002] Bioprostheses are biological materials used in the treatment of heart disease. The main raw material of the current biological valve valve is the pericardium, and the main reason for limiting the durability of the biological heart valve is that the pericardium is easily calcified and the valve fails. Pericardial calcification is mainly due to calcification sites that are easy to react with calcium ions, such as pericardial phospholipids, residual cells and cell debris, and exposed amino groups, carboxyl groups, and aldehyde groups in the pericardial molecular structure. In addition, the valve is damaged during multiple opening and closing processes, resulting in exposure of functional groups and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02A61L27/36C11D1/04C11D1/74C11D3/20C11D3/60C11D7/26C11D7/32C11D7/60
CPCA01N1/0231A01N1/0215A61L27/3625A61L27/3687C11D1/74C11D1/04C11D3/2006C11D3/2017C11D3/2068C11D7/3245C11D7/263C11D7/261C11D11/0017A61L2430/20A61L2430/40
Inventor 尚大鹏左一聪杨夏燕虞奇峰秦涛
Owner SHANGHAI NEWMED MEDICAL CO LTD
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