Methods for treating cancer resistant to cdk4/6 inhibitors
A technology of drug resistance and inhibitors, applied in pharmaceutical formulations, biochemical equipment and methods, drug combinations, etc., can solve the problems of patients with relapsed drug-resistant breast cancer
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[0132] Erastran used in the following examples is (6R)-6-(2-(N-(4-(2-(ethylamino)ethyl)benzyl)-N-ethylamino)-4- Methoxyphenyl)-5,6,7,8-tetralin-2-ol dihydrochloride ((6R)-6-(2-(N-(4-(2-(ethylamino)ethyl) benzyl)-N-ethylamino)-4-methoxyphenyl)-5,6,7,8-tetrahydronaphthalen-2-ol dihydrochloride), for example manufactured by IRIX Pharmaceuticals Ltd. (Florence, SC). Erastrant is stored in the form of dry powder, prepared into a homogeneous suspension with deionized water containing 0.5% (w / v) methylcellulose, and administered orally to animal models. Tamoxifen, raloxifene, and estradiol (E2) were obtained from Sigma-Aldrich (St. Louis, MO) and administered by subcutaneous injection. Fulvestrant was obtained from Tocris Biosciences (Minneapolis, MN) and administered by subcutaneous injection. Other laboratory reagents were purchased from Sigma-Aldrich unless otherwise stated.
[0133] Development of resistance to CDK4 / 6...
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