Yeast-based compositions for enhancing rhizosphere properties and plant health
A health status, compositional technology, applied in the fields of soil treatment compositions, microbial-based products, cultured microorganisms and/or growth by-products, capable of addressing complex, sustainable burdens, and difficulties dealing with a wide range of plant species
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[0147] Preparation of Microbial-Based Products
[0148] A microorganism-based product of the present invention is simply the fermentation medium containing the microorganisms and / or microbial metabolites and / or any residual nutrients produced by the microorganisms. Fermented products are used directly without extraction or purification. Extraction and purification can be readily achieved, if desired, using standard extraction and / or purification methods or techniques described in the literature.
[0149] Microorganisms in microbial-based products may be in active or inactive form, or in the form of vegetative cells, germ spores, mycelium, hyphae, conidia or any other form of microbial propagules. Microorganism-based products may also contain combinations of these forms of microorganisms.
[0150] In one embodiment, different microbial strains are cultured separately and then mixed together to produce a microbial-based product. Optionally, the microorganism may be mixed with...
Embodiment 1
[0239] Example 1 - Solid State Fermentation of Abnormal Wickham's Saccharomyces
[0240] For biomass production with Saccharomyces cerevisiae strains, rice medium was used. About 200 g of rice was mixed with 600 ml of GUY medium (glucose, urea and yeast extract, pH 5.71) or 250 ml of concentrated GY medium (glucose and yeast extract, pH 5.69) and water. Spread the medium in a layer approximately 1 to 2 inches thick on a stainless steel dish and sterilize.
[0241] After sterilization, plates were inoculated with seed cultures. Optionally, added nutrients may be included to enhance microbial growth including, for example, salts and / or carbon sources such as molasses, starch, glucose and sucrose.
[0242] A seed culture of Wickhamiana anomalies was then sprayed or pipetted onto the substrate surface and the trays were incubated in a closed reactor at 28-30°C. Ambient air was pumped into the reactor to allow the temperature to stabilize. 48-72 hours of culture can produce 1×1...
Embodiment 2
[0243] Example 2 - Preparation of Microbial Based Products
[0244] A resealable bag can be used to store and transport a product containing at least 1 x 10 8 Abnormal Wickham yeast product in CFU / ml. Other compounds such as micronutrients, macronutrients, prebiotics and / or other similarly produced microorganisms can be added to the product.
[0245] Then, add water to the mixing tank to dilute to 1x10 6 to 1x10 7 Concentration of CFU / ml. One bag of product can treat approximately 1-10 acres of crops or citrus groves.
[0246] The composition may be mixed and / or applied simultaneously with additional "inoculum" material to promote initial growth of microorganisms in the composition. These can include things like prebiotics and / or nanofertilizers (e.g., Aqua-Yield, NanoGro TM )Wait.
[0247] An exemplary formulation of an inoculum composition comprises:
[0248] Soluble Potassium (K2O) (1.0% to 2.5%, or about 2.0%)
[0249] Magnesium (Mg) (0.25% to 0.75%, or about 0.5%...
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