Method for detecting cannabinoid in industrial cannabis sativa flowers and leaves based on UPLC-MSMS

A technology for industrial hemp, cannabinoids, applied in the field of bioanalysis

Pending Publication Date: 2021-07-30
黑龙江省农业科学院农产品质量安全研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no unified standard and method for the detection of CBGA, CBG, CBC and CBCA content in industrial hemp flowers and leaves in China.

Method used

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  • Method for detecting cannabinoid in industrial cannabis sativa flowers and leaves based on UPLC-MSMS
  • Method for detecting cannabinoid in industrial cannabis sativa flowers and leaves based on UPLC-MSMS
  • Method for detecting cannabinoid in industrial cannabis sativa flowers and leaves based on UPLC-MSMS

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Experimental program
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Embodiment 1

[0040] (1) Pretreatment

[0041] The collected leaves, leaves, seeds, and stems are ventilated and dried in the shade. After removing impurities, put them in a grinder and grind them, pass through a 50-mesh sieve to make samples, and store them at -18°C; according to GB 5009.3 Determine the water content in the sample and calculate the dry matter content in the sample;

[0042] (2) Extraction and purification

[0043] Accurately weigh 0.1-1g of the sample to be tested, accurate to at least 0.01g, put it into a 50mL centrifuge tube, add 25mL of 90+10 methanol-n-hexane solution to the centrifuge tube, vortex extract for 15min, and centrifuge at 5000r / min for 3min; Transfer all the supernatant to a 50mL volumetric flask, add 25mL of 90+10 methanol-n-hexane solution to the residue again, vortex extract for 15min, centrifuge at 5000r / min for 3min, combine the extracts, dilute to 50mL, and mix well Take 2mL of the mixture and filter it through a 0.22μm organic filter;

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Abstract

The invention relates to the technical field of biological analysis, in particular to a method for detecting the content of CBGA, CBG, CBC and CBCA in industrial hemp floral leaves based on UPLC-MSMS, the UPLC-MSMS is used for detecting the deviation between the retention time of an object to be detected in a sample and the retention time of an object to be detected in a standard working solution, and whether the object to be detected contains a target object or not is qualitatively analyzed. Quantitative determination is performed on a sample solution containing a target object. Quantifying is performed by an external standard method, the monitoring ion peak area of the object to be detected is detected by UPLC-MSMS, comparing is performed with the standard curve, and the concentration of the target object in the sample solution is calculated. The accuracy and precision of the method are verified by adding the recovery rate and the relative standard deviation RSD, which shows that the method has good extraction efficiency on CBGA, CBG, CBC and CBCA, and can meet the analysis test of CBGA, CBG, CBC and CBCA in industrial cannabis sativa.

Description

technical field [0001] The invention relates to the technical field of bioanalysis, in particular to a method for detecting cannabigerolic acid (CBGA), cannabigerol (CBG), cannabichromene (CBC) and cannabichromene in industrial hemp flowers and leaves based on UPLC-MSMS acid (CBCA) method. Background technique [0002] The planting area of ​​hemp in Heilongjiang Province reaches 457,000 mu. According to the plan of the National Hemp Strategic Industry Alliance and the national hemp planting arrangement made by the Hemp Research Center, Heilongjiang Province initially plans to reach 3 million mu in about 10 years. [0003] About 500 compounds have been identified from cannabis so far. Among the more than 500 compounds, cannabinoids have attracted the most attention from researchers. Cannabinoids are characteristic terpene-phenolic compounds concentrated in resinous secretions produced by plant trichomes. These trichomes are mainly concentrated in specific parts of the fema...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈国峰刘峰张晓波董见南黄文功廖辉张瑞英程爱华史冬梅金海涛任红波马文琼潘博李宛孙丽荣尤红梅孙悦萍
Owner 黑龙江省农业科学院农产品质量安全研究所
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