Target MLLT11 for preventing lung cancer and application thereof

A lung cancer and target technology, applied in the biological field, to achieve the effect of inhibiting malignant transformation and preventing lung cancer

Active Publication Date: 2021-08-10
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Erdafitinib (FGFR inhibitor), Avelumab (targeting PD-L1 drug), Tuoyi and other drugs targeting bladder cancer cannot effectively treat lung cancer

Method used

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  • Target MLLT11 for preventing lung cancer and application thereof
  • Target MLLT11 for preventing lung cancer and application thereof
  • Target MLLT11 for preventing lung cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Normal lung bronchial epithelial cells were treated with arsenic, and the protein expression level of MLLT11 was up-regulated in a concentration- and time-dependent manner

[0047] 1. Cell infection treatment

[0048] (1) Treatment of acute arsenic exposure of cells:

[0049] a) Count the cells of human normal bronchial epithelial cells BEAS-2B. A total of 200,000 cells are spread in each well of a six-well plate, with a total of 8 wells. Using DMEM medium containing 10% FBS, keep the temperature at 37°C in CO 2 The culture was carried out in an incubator; the cell density reached about 80% after 12 hours.

[0050] b) 12 hours after seeding the plate, the original DMEM medium containing 10% FBS was replaced with 0.1% FBS DMEM medium, and the cells were starved for 12 hours according to the same culture conditions as above.

[0051] c) After the cells were starved for 12 hours, two groups of experiments were carried out, each with 4 wells of cells. One grou...

Embodiment 2

[0071] Example 2. The expression level of MLLT11 in clinical lung cancer tissues generally shows a high trend.

[0072] 1. Clinical tissue immunohistochemistry

[0073] (1) Paraffin-embedded sections:

[0074] a) Tissue collection: Lung cancer tissue and its corresponding paracancerous tissue were collected. This operation strictly abides by the relevant regulations of the Medical Ethics Committee of Wenzhou Medical University.

[0075] b) Tissue fixation: lung cancer tissue was fixed with 4% PFA, and fixed at 4°C for 24-48h.

[0076] c) Tissue dehydration: the fixed lung cancer tissue was washed with running water, and the PFA was cleaned. Then put the specimen in 30% ethanol for 30min, 50% ethanol for 30min, 70% ethanol for 1h, 80% ethanol for 1h, 85% ethanol for 1h, 95% ethanol for 1h, 95% ethanol for 1h, 100% ethanol for 1h, 100% ethanol for 1h gradient dehydration.

[0077] d) Tissue transparency: After the tissue is dehydrated, put the tissue in a solution mixed wit...

Embodiment 3

[0101] Example 3, MLLT11 significantly promotes the colony formation of BEAS-2B cells induced by arsenide for a long time.

[0102] 1. Steps related to cell transfection (1) Lipofectamine transfection method:

[0103] a) BEAS-2B cells were plated for 24 hours, and when the cells grew to 80%, the medium was replaced 1 hour in advance with fresh 10% FBSDMEM+++ medium.

[0104] b) Add 2 μg of MLLT11 KO plasmid (Vector, KO#1, KO#2, KO#3) into 50 μL of serum-free DMEM containing high glucose and mix thoroughly.

[0105] c) Take 6μL PolyJet TM Add 50 μL of serum-free DMEM containing high glucose to the reagent, and mix gently with a gun for 3-4 times.

[0106] d) Immediately place the diluted PolyJet TM The reagent was added to the diluted DNA solution, and mixed up and down gently for 3-4 times. (sequence only PolyJet TM plus DNA)

[0107] e) Incubate at room temperature for 15 minutes, not exceeding 20 minutes.

[0108] f) Dispense 100μL PolyJet TM Add the DNA mixture drop...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to MLLT11 serving as a new target for preventing and diagnosing arsenide-induced lung cancer and application of the MLLT11. After the MLLT11 is knocked out, the inhibition effect of arsenide-induced normal bronchial epithelial cells of the lung can be remarkably inhibited. The MLLT11 has a potential prevention effect on lung cancer, at least lung cancer induced by arsenide; and therefore, the MLLT11 is expected to further play a role in preventing the lung cancer clinically.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to MLLT11 as a new target for preventing and diagnosing arsenic-induced lung cancer and its application. Background technique [0002] According to the latest global cancer statistics released by the World Health Organization in 2020, there are still nearly 2.2 million new cases of lung cancer and 1.8 million deaths from lung cancer in the world, which seriously threatens the life and health of all human beings. Among them, the data on the incidence of cancer in China in 2020 shows that the incidence of lung cancer is 17.9%, and the mortality rate is as high as 23.8%. Therefore, the prevention and treatment of lung cancer is urgent for China and the world. Arsenic is a non-metallic element that widely exists in nature and is widely used in people's production and life, including pesticides, insecticides, herbicides and alloys. However, arsenic and its compounds are like a d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P35/00
CPCA61K45/00A61P35/00
Inventor 赵玲玲赵贺金红蕾黄海山
Owner WENZHOU MEDICAL UNIV
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